Iron in PDB 2v27: Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H

All present enzymatic activity of Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H:
1.14.16.1;

The structure of Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H, PDB code: 2v27 was solved by H.-K.S.Leiros, A.L.Pey, M.Innselset, E.Moe, I.Leiros, I.H.Steen, A.Martinez, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	40.280, 86.020, 87.570, 90.00, 97.01, 90.00
R / Rfree (%)	16.2 / 19.5

The binding sites of Iron atom in the Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H (pdb code 2v27). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H, PDB code: 2v27:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1268 b:12.3 occ:1.00 NE2 A:HIS127 2.1 13.3 1.0 NE2 A:HIS122 2.1 15.1 1.0 O A:HOH2273 2.1 25.2 1.0 O A:HOH2231 2.2 15.1 1.0 OE1 A:GLU167 2.2 14.6 1.0 OE2 A:GLU167 2.4 12.7 1.0 CD A:GLU167 2.6 11.2 1.0 CE1 A:HIS122 3.0 13.3 1.0 CD2 A:HIS127 3.0 13.4 1.0 CE1 A:HIS127 3.1 11.2 1.0 CD2 A:HIS122 3.2 11.5 1.0 ND1 A:HIS122 4.1 14.0 1.0 ND1 A:HIS127 4.2 12.7 1.0 CG A:HIS127 4.2 10.7 1.0 CG A:GLU167 4.2 9.0 1.0 OE1 A:GLU123 4.2 13.6 1.0 CG A:HIS122 4.3 10.3 1.0 O A:HOH2270 4.3 29.8 1.0 O A:HOH2230 4.4 41.8 1.0 O A:HOH2272 4.5 28.0 1.0 O A:HOH2138 4.7 44.6 1.0 N A:GLY183 4.8 7.1 1.0 O A:HOH2100 4.9 36.3 1.0 CB A:PRO118 5.0 16.5 1.0

Iron binding site 2 out of 2 in the Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structure of the Cold Active Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe3008 b:10.7 occ:1.00 NE2 B:HIS122 2.0 14.2 1.0 O B:HOH2201 2.1 13.9 1.0 OE1 B:GLU167 2.1 13.6 1.0 NE2 B:HIS127 2.2 11.7 1.0 O B:HOH2357 2.3 21.1 1.0 OE2 B:GLU167 2.3 13.3 1.0 CD B:GLU167 2.5 11.4 1.0 CE1 B:HIS122 2.9 17.8 1.0 CD2 B:HIS127 3.1 9.2 1.0 CD2 B:HIS122 3.1 14.3 1.0 CE1 B:HIS127 3.2 12.7 1.0 OE1 B:GLU123 4.1 12.5 1.0 ND1 B:HIS122 4.1 16.9 1.0 CG B:GLU167 4.1 10.3 1.0 O B:HIS1007 4.1 16.8 1.0 O B:HOH2356 4.1 18.0 1.0 CG B:HIS122 4.2 10.6 1.0 CG B:HIS127 4.2 10.7 1.0 ND1 B:HIS127 4.3 11.4 1.0 O B:HOH2236 4.5 36.5 1.0 N B:GLY183 4.8 7.7 1.0 C B:HIS1007 4.8 16.8 1.0 CD B:GLU123 4.9 13.1 1.0 CB B:GLU167 4.9 8.0 1.0 O B:HOH2200 5.0 30.6 1.0

H.-K.S.Leiros, A.L.Pey, M.Innselset, E.Moe, I.Leiros, I.H.Steen, A.Martinez. Structure of Phenylalanine Hydroxylase From Colwellia Psychrerythraea 34H, A Monomeric Cold Active Enzyme with Local Flexibility Around the Active Site and High Overall Stability. J.Biol.Chem. V. 282 21973 2007.
ISSN: ISSN 0021-9258
PubMed: 17537732
DOI: 10.1074/JBC.M610174200