Iron in PDB 3bcc: Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken

All present enzymatic activity of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken:
1.10.2.2;

The structure of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken, PDB code: 3bcc was solved by Z.Zhang, L.Huang, V.M.Shulmeister, Y.-I.Chi, K.K.Kim, L.-W.Hung, A.R.Crofts, E.A.Berry, S.-H.Kim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	12.00 / 3.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	173.180, 179.730, 238.220, 90.00, 90.00, 90.00
R / Rfree (%)	28.9 / 32.1

The binding sites of Iron atom in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken (pdb code 3bcc). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 5 binding sites of Iron where determined in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken, PDB code: 3bcc:
Jump to Iron binding site number: 1; 2; 3; 4; 5;

Iron binding site 1 out of 5 in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe381 b:28.6 occ:1.00 FE C:HEM381 0.0 28.6 1.0 NA C:HEM381 1.7 31.8 1.0 NB C:HEM381 1.8 31.2 1.0 NC C:HEM381 1.8 37.5 1.0 ND C:HEM381 1.9 33.2 1.0 NE2 C:HIS183 2.0 27.4 1.0 NE2 C:HIS84 2.1 37.9 1.0 C1A C:HEM381 2.8 34.5 1.0 C4A C:HEM381 2.8 30.6 1.0 C4B C:HEM381 2.9 36.3 1.0 CE1 C:HIS84 2.9 43.6 1.0 C1C C:HEM381 2.9 42.1 1.0 C1B C:HEM381 2.9 34.3 1.0 CE1 C:HIS183 2.9 29.5 1.0 C4D C:HEM381 2.9 35.1 1.0 C4C C:HEM381 3.0 39.3 1.0 C1D C:HEM381 3.0 34.5 1.0 CD2 C:HIS183 3.1 26.4 1.0 CD2 C:HIS84 3.2 37.6 1.0 CHA C:HEM381 3.2 37.9 1.0 CHC C:HEM381 3.2 41.0 1.0 CHB C:HEM381 3.2 32.6 1.0 CHD C:HEM381 3.5 34.9 1.0 C3A C:HEM381 4.0 32.1 1.0 C2A C:HEM381 4.0 33.6 1.0 ND1 C:HIS84 4.1 43.9 1.0 ND1 C:HIS183 4.1 30.3 1.0 C3B C:HEM381 4.1 37.4 1.0 C2B C:HEM381 4.1 34.8 1.0 CG C:HIS183 4.2 26.2 1.0 C2C C:HEM381 4.2 42.9 1.0 C3D C:HEM381 4.2 35.1 1.0 C3C C:HEM381 4.2 42.4 1.0 C2D C:HEM381 4.2 37.2 1.0 CG C:HIS84 4.3 39.1 1.0 CA C:GLY131 4.9 44.4 1.0 CA C:GLY49 4.9 28.2 1.0

Iron binding site 2 out of 5 in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe382 b:30.4 occ:1.00 FE C:HEM382 0.0 30.4 1.0 NA C:HEM382 1.8 24.8 1.0 ND C:HEM382 1.8 35.1 1.0 NB C:HEM382 1.9 30.8 1.0 NC C:HEM382 1.9 30.3 1.0 NE2 C:HIS197 2.1 35.2 1.0 NE2 C:HIS98 2.2 31.9 1.0 C4A C:HEM382 2.8 25.4 1.0 C1A C:HEM382 2.9 25.7 1.0 C1D C:HEM382 2.9 41.3 1.0 C1B C:HEM382 2.9 34.6 1.0 C4D C:HEM382 2.9 32.8 1.0 C1C C:HEM382 3.0 32.1 1.0 C4C C:HEM382 3.0 29.5 1.0 C4B C:HEM382 3.0 33.1 1.0 CE1 C:HIS197 3.0 37.7 1.0 CD2 C:HIS197 3.1 38.4 1.0 CE1 C:HIS98 3.1 35.9 1.0 CD2 C:HIS98 3.2 32.2 1.0 CHB C:HEM382 3.2 33.5 1.0 CHA C:HEM382 3.3 29.5 1.0 CHD C:HEM382 3.3 36.8 1.0 CHC C:HEM382 3.4 34.1 1.0 C3A C:HEM382 4.0 25.5 1.0 ND1 C:HIS197 4.1 41.1 1.0 C2A C:HEM382 4.1 26.6 1.0 C2D C:HEM382 4.1 43.1 1.0 CG C:HIS197 4.2 38.9 1.0 C3D C:HEM382 4.2 36.8 1.0 C2B C:HEM382 4.2 35.5 1.0 C3B C:HEM382 4.2 35.5 1.0 C2C C:HEM382 4.2 32.9 1.0 C3C C:HEM382 4.2 28.6 1.0 ND1 C:HIS98 4.3 37.9 1.0 CG C:HIS98 4.3 32.9 1.0

Iron binding site 3 out of 5 in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe242 b:40.5 occ:1.00 FE D:HEM242 0.0 40.5 1.0 NC D:HEM242 1.9 42.8 1.0 ND D:HEM242 1.9 39.6 1.0 NE2 D:HIS41 1.9 47.9 1.0 NA D:HEM242 1.9 39.2 1.0 NB D:HEM242 2.0 39.0 1.0 SD D:MET160 2.1 45.5 1.0 CE1 D:HIS41 2.9 56.4 1.0 C4C D:HEM242 2.9 44.5 1.0 C1D D:HEM242 2.9 40.4 1.0 CD2 D:HIS41 2.9 56.9 1.0 C1B D:HEM242 3.0 39.5 1.0 C1C D:HEM242 3.0 43.5 1.0 C4D D:HEM242 3.0 40.3 1.0 C4A D:HEM242 3.0 36.5 1.0 C1A D:HEM242 3.0 37.4 1.0 C4B D:HEM242 3.1 39.0 1.0 CHD D:HEM242 3.3 42.4 1.0 CHB D:HEM242 3.3 35.3 1.0 CHA D:HEM242 3.3 40.5 1.0 CHC D:HEM242 3.4 39.8 1.0 CE D:MET160 3.4 44.9 1.0 CG D:MET160 3.5 52.3 1.0 ND1 D:HIS41 4.0 62.0 1.0 CG D:HIS41 4.0 59.1 1.0 C2D D:HEM242 4.2 42.5 1.0 C3C D:HEM242 4.2 44.6 1.0 CB D:MET160 4.2 57.5 1.0 C2C D:HEM242 4.2 42.9 1.0 C2B D:HEM242 4.2 41.9 1.0 C3D D:HEM242 4.2 41.8 1.0 C3A D:HEM242 4.2 35.2 1.0 C2A D:HEM242 4.2 35.7 1.0 C3B D:HEM242 4.2 40.9 1.0

Iron binding site 4 out of 5 in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken within 5.0Å range: probe atom residue distance (Å) B Occ E:Fe197 b:71.2 occ:1.00 FE1 E:FES197 0.0 71.2 1.0 S1 E:FES197 2.2 87.7 1.0 S2 E:FES197 2.2 88.2 1.0 SG E:CYS158 2.2 74.3 1.0 SG E:CYS139 2.5 67.7 1.0 FE2 E:FES197 2.6 84.3 1.0 CB E:CYS158 2.9 75.8 1.0 CB E:CYS139 3.3 67.5 1.0 CB E:HIS141 3.9 72.5 1.0 CB E:CYS160 4.2 79.6 1.0 OG E:SER163 4.2 67.5 1.0 CB E:SER163 4.3 76.8 1.0 CA E:CYS158 4.3 80.1 1.0 O E:CYS158 4.3 85.4 1.0 N E:HIS161 4.5 83.0 1.0 ND1 E:HIS161 4.5 73.4 1.0 CB E:CYS144 4.5 74.4 1.0 C E:CYS158 4.6 80.2 1.0 ND1 E:HIS141 4.7 68.1 1.0 CG E:HIS141 4.7 74.4 1.0 CA E:CYS139 4.8 69.2 1.0 N E:HIS141 4.9 77.3 1.0 N E:CYS144 4.9 75.9 1.0 N E:LEU142 4.9 76.1 1.0 CA E:HIS141 4.9 75.8 1.0 N E:SER163 5.0 83.2 1.0 CB E:HIS161 5.0 80.3 1.0 N E:CYS160 5.0 77.9 1.0

Iron binding site 5 out of 5 in the Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Stigmatellin and Antimycin Bound Cytochrome BC1 Complex From Chicken within 5.0Å range: probe atom residue distance (Å) B Occ E:Fe197 b:84.3 occ:1.00 FE2 E:FES197 0.0 84.3 1.0 S2 E:FES197 2.2 88.2 1.0 ND1 E:HIS161 2.2 73.4 1.0 S1 E:FES197 2.2 87.7 1.0 ND1 E:HIS141 2.3 68.1 1.0 FE1 E:FES197 2.6 71.2 1.0 CG E:HIS141 3.0 74.4 1.0 CB E:HIS141 3.0 72.5 1.0 CG E:HIS161 3.0 77.0 1.0 CB E:HIS161 3.1 80.3 1.0 CE1 E:HIS161 3.3 75.2 1.0 CE1 E:HIS141 3.5 68.4 1.0 N E:HIS161 3.7 83.0 1.0 CB E:CYS160 3.9 79.6 1.0 CA E:HIS161 4.0 83.9 1.0 N E:LEU142 4.0 76.1 1.0 CD2 E:HIS141 4.2 74.7 1.0 SG E:CYS139 4.2 67.7 1.0 CD2 E:HIS161 4.2 76.9 1.0 C E:CYS160 4.3 81.9 1.0 NE2 E:HIS161 4.3 75.2 1.0 SG E:CYS158 4.3 74.3 1.0 NE2 E:HIS141 4.4 72.8 1.0 CA E:HIS141 4.4 75.8 1.0 CB E:LEU142 4.6 80.1 1.0 C E:HIS141 4.7 75.9 1.0 CA E:CYS160 4.7 81.0 1.0 OG E:SER163 4.7 67.5 1.0 C E:HIS161 4.8 85.8 1.0 CA E:LEU142 4.9 79.1 1.0 CG E:LEU142 4.9 84.8 1.0 O E:CYS160 5.0 78.5 1.0

Z.Zhang, L.Huang, V.M.Shulmeister, Y.I.Chi, K.K.Kim, L.W.Hung, A.R.Crofts, E.A.Berry, S.H.Kim. Electron Transfer By Domain Movement in Cytochrome BC1. Nature V. 392 677 1998.
ISSN: ISSN 0028-0836
PubMed: 9565029
DOI: 10.1038/33612