Iron in PDB 3fsn: Crystal Structure of RPE65 at 2.14 Angstrom Resolution

All present enzymatic activity of Crystal Structure of RPE65 at 2.14 Angstrom Resolution:
5.2.1.7;

The structure of Crystal Structure of RPE65 at 2.14 Angstrom Resolution, PDB code: 3fsn was solved by P.D.Kiser, D.T.Lodowski, K.Palczewski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.22 / 2.14
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	176.516, 176.516, 86.862, 90.00, 90.00, 120.00
R / Rfree (%)	18 / 21.6

The binding sites of Iron atom in the Crystal Structure of RPE65 at 2.14 Angstrom Resolution (pdb code 3fsn). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of RPE65 at 2.14 Angstrom Resolution, PDB code: 3fsn:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of RPE65 at 2.14 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of RPE65 at 2.14 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe534 b:28.1 occ:1.00 NE2 A:HIS527 2.2 32.2 1.0 NE2 A:HIS241 2.2 35.9 1.0 NE2 A:HIS313 2.2 33.1 1.0 NE2 A:HIS180 2.3 32.9 1.0 CD2 A:HIS527 3.1 33.0 1.0 CD2 A:HIS313 3.1 33.8 1.0 CE1 A:HIS241 3.1 37.7 1.0 CE1 A:HIS527 3.1 33.8 1.0 CD2 A:HIS241 3.2 34.6 1.0 CE1 A:HIS313 3.2 33.1 1.0 CE1 A:HIS180 3.2 34.8 1.0 CD2 A:HIS180 3.2 31.2 1.0 ND1 A:HIS527 4.2 32.5 1.0 CG A:HIS527 4.2 33.0 1.0 ND1 A:HIS241 4.2 35.2 1.0 CG A:HIS313 4.3 33.2 1.0 ND1 A:HIS313 4.3 35.1 1.0 CG A:HIS241 4.3 33.0 1.0 ND1 A:HIS180 4.3 34.7 1.0 CG A:HIS180 4.4 34.3 1.0 CG1 A:VAL134 4.9 32.0 1.0 CE1 A:PHE418 5.0 36.8 1.0

Iron binding site 2 out of 2 in the Crystal Structure of RPE65 at 2.14 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of RPE65 at 2.14 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe534 b:26.0 occ:1.00 NE2 B:HIS180 2.1 34.9 1.0 NE2 B:HIS241 2.2 35.4 1.0 NE2 B:HIS313 2.2 30.8 1.0 NE2 B:HIS527 2.2 31.1 1.0 CE1 B:HIS180 3.1 36.7 1.0 CE1 B:HIS241 3.1 37.1 1.0 CD2 B:HIS527 3.1 31.6 1.0 CE1 B:HIS527 3.1 33.2 1.0 CD2 B:HIS313 3.1 33.0 1.0 CE1 B:HIS313 3.1 31.2 1.0 CD2 B:HIS180 3.2 33.7 1.0 CD2 B:HIS241 3.2 36.2 1.0 ND1 B:HIS527 4.2 33.2 1.0 CG B:HIS527 4.2 34.2 1.0 ND1 B:HIS180 4.2 35.4 1.0 ND1 B:HIS313 4.2 34.9 1.0 ND1 B:HIS241 4.2 35.6 1.0 CG B:HIS313 4.2 33.2 1.0 CG B:HIS180 4.3 35.0 1.0 CG B:HIS241 4.3 33.4 1.0 CG1 B:VAL134 4.9 33.5 1.0

P.D.Kiser, M.Golczak, D.T.Lodowski, M.R.Chance, K.Palczewski. Crystal Structure of Native RPE65, the Retinoid Isomerase of the Visual Cycle. Proc.Natl.Acad.Sci.Usa V. 106 17325 2009.
ISSN: ISSN 0027-8424
PubMed: 19805034
DOI: 10.1073/PNAS.0906600106