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Iron in PDB 3fwf: Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form

Enzymatic activity of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form

All present enzymatic activity of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form:
1.14.15.1;

Protein crystallography data

The structure of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form, PDB code: 3fwf was solved by I.Schlichting, K.Von Koenig, C.Aldag, D.Hilvert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.64 / 1.83
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 67.520, 61.930, 94.460, 90.00, 90.80, 90.00
R / Rfree (%) 16.2 / 20.7

Other elements in 3fwf:

The structure of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form also contains other interesting chemical elements:

Potassium (K) 4 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form (pdb code 3fwf). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form, PDB code: 3fwf:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 3fwf

Go back to Iron Binding Sites List in 3fwf
Iron binding site 1 out of 2 in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe417

b:13.9
occ:1.00
FE A:HEM417 0.0 13.9 1.0
NB A:HEM417 2.1 13.2 1.0
NC A:HEM417 2.1 13.8 1.0
NA A:HEM417 2.1 13.3 1.0
ND A:HEM417 2.1 13.1 1.0
SE A:SEC357 2.5 18.3 1.0
C4B A:HEM417 3.0 11.8 1.0
C1C A:HEM417 3.1 13.2 1.0
C1A A:HEM417 3.1 13.4 1.0
C4A A:HEM417 3.1 11.4 1.0
C1B A:HEM417 3.1 11.4 1.0
C1D A:HEM417 3.1 13.4 1.0
C4D A:HEM417 3.1 12.8 1.0
C4C A:HEM417 3.1 13.0 1.0
CHC A:HEM417 3.4 11.4 1.0
CB A:SEC357 3.4 14.6 1.0
CHA A:HEM417 3.4 13.4 1.0
CHB A:HEM417 3.5 11.8 1.0
CHD A:HEM417 3.5 13.1 1.0
CA A:SEC357 4.1 14.9 1.0
C5 A:CAM420 4.2 19.4 1.0
C3B A:HEM417 4.3 12.7 1.0
C2C A:HEM417 4.3 13.6 1.0
C3A A:HEM417 4.3 12.8 1.0
C2B A:HEM417 4.3 13.5 1.0
C3C A:HEM417 4.3 13.1 1.0
C2A A:HEM417 4.3 13.7 1.0
C3D A:HEM417 4.3 13.6 1.0
C2D A:HEM417 4.3 14.7 1.0
C4 A:CAM420 4.5 19.5 1.0
C9 A:CAM420 4.6 20.5 1.0
N A:GLY359 4.8 15.9 1.0
C A:SEC357 4.9 15.3 1.0
N A:LEU358 4.9 15.3 1.0

Iron binding site 2 out of 2 in 3fwf

Go back to Iron Binding Sites List in 3fwf
Iron binding site 2 out of 2 in the Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Ferric Camphor Bound Cytochrome P450CAM Containing A Selenocysteine As the 5TH Heme Ligand, Monoclinic Crystal Form within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe417

b:11.6
occ:1.00
FE B:HEM417 0.0 11.6 1.0
NB B:HEM417 2.1 10.8 1.0
NA B:HEM417 2.1 9.2 1.0
ND B:HEM417 2.1 11.4 1.0
NC B:HEM417 2.1 11.8 1.0
SE B:SEC357 2.5 15.8 1.0
C4B B:HEM417 3.0 10.8 1.0
C4A B:HEM417 3.1 8.2 1.0
C1C B:HEM417 3.1 11.1 1.0
C4D B:HEM417 3.1 11.6 1.0
C1B B:HEM417 3.1 9.4 1.0
C1A B:HEM417 3.1 10.3 1.0
C1D B:HEM417 3.1 11.3 1.0
C4C B:HEM417 3.2 11.5 1.0
CHC B:HEM417 3.4 9.2 1.0
CB B:SEC357 3.4 13.2 1.0
CHB B:HEM417 3.4 8.0 1.0
CHA B:HEM417 3.5 10.0 1.0
CHD B:HEM417 3.5 11.8 1.0
CA B:SEC357 4.1 13.2 1.0
C5 B:CAM420 4.1 15.2 1.0
C3B B:HEM417 4.3 8.6 1.0
C3A B:HEM417 4.3 8.2 1.0
C2B B:HEM417 4.3 9.8 1.0
C2A B:HEM417 4.3 9.7 1.0
C2C B:HEM417 4.3 12.0 1.0
C3D B:HEM417 4.3 11.4 1.0
C2D B:HEM417 4.3 12.0 1.0
C3C B:HEM417 4.4 13.1 1.0
C4 B:CAM420 4.6 14.9 1.0
C9 B:CAM420 4.8 11.9 1.0
N B:LEU358 4.8 14.1 1.0
N B:GLY359 4.8 15.2 1.0
C B:SEC357 4.8 13.7 1.0

Reference:

C.Aldag, I.A.Gromov, I.Garcia-Rubio, K.Von Koenig, I.Schlichting, B.Jaun, D.Hilvert. Probing the Role of the Proximal Heme Ligand in Cytochrome P450CAM By Recombinant Incorporation of Selenocysteine. Proc.Natl.Acad.Sci.Usa V. 106 5481 2009.
ISSN: ISSN 0027-8424
PubMed: 19293375
DOI: 10.1073/PNAS.0810503106
Page generated: Sun Aug 4 10:18:43 2024

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