Iron in PDB 3kvc: Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution

All present enzymatic activity of Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution:
3.1.1.64; 5.2.1.7;

The structure of Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution, PDB code: 3kvc was solved by P.D.Kiser, D.T.Lodowski, M.Golczak, K.Palczewski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	176.356, 176.356, 86.715, 90.00, 90.00, 120.00
R / Rfree (%)	14.6 / 17.1

The binding sites of Iron atom in the Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution (pdb code 3kvc). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution, PDB code: 3kvc:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe534 b:22.4 occ:1.00 NE2 A:HIS313 2.1 19.6 1.0 NE2 A:HIS527 2.1 21.9 1.0 NE2 A:HIS180 2.1 18.9 1.0 NE2 A:HIS241 2.1 22.0 1.0 CD2 A:HIS313 2.9 20.0 1.0 CE1 A:HIS527 3.0 22.5 1.0 CE1 A:HIS180 3.0 22.4 1.0 CD2 A:HIS527 3.1 19.5 1.0 CE1 A:HIS241 3.1 24.8 1.0 CD2 A:HIS241 3.1 22.2 1.0 CD2 A:HIS180 3.2 23.1 1.0 CE1 A:HIS313 3.2 23.8 1.0 ND1 A:HIS527 4.0 21.6 1.0 CG A:HIS527 4.1 21.8 1.0 CG A:HIS313 4.2 19.6 1.0 ND1 A:HIS180 4.2 22.8 1.0 ND1 A:HIS313 4.2 16.0 1.0 ND1 A:HIS241 4.2 24.9 1.0 CG A:HIS180 4.3 20.1 1.0 CG A:HIS241 4.3 21.7 1.0 CE1 A:PHE418 4.9 28.6 1.0 CG1 A:VAL134 4.9 23.0 1.0

Iron binding site 2 out of 2 in the Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Bovine RPE65 at 1.9 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe534 b:21.2 occ:1.00 NE2 B:HIS527 2.1 18.8 1.0 NE2 B:HIS180 2.1 21.2 1.0 NE2 B:HIS313 2.1 19.3 1.0 NE2 B:HIS241 2.2 19.0 1.0 CE1 B:HIS527 3.0 17.0 1.0 CD2 B:HIS313 3.0 17.7 1.0 CE1 B:HIS180 3.1 20.5 1.0 CE1 B:HIS241 3.1 24.0 1.0 CD2 B:HIS527 3.1 19.0 1.0 CD2 B:HIS180 3.2 18.4 1.0 CD2 B:HIS241 3.2 18.2 1.0 CE1 B:HIS313 3.2 20.7 1.0 ND1 B:HIS527 4.1 20.6 1.0 CG B:HIS527 4.1 19.6 1.0 CG B:HIS313 4.2 18.3 1.0 ND1 B:HIS180 4.2 22.6 1.0 ND1 B:HIS241 4.2 24.3 1.0 ND1 B:HIS313 4.3 15.0 1.0 CG B:HIS180 4.3 19.6 1.0 CG B:HIS241 4.3 19.4 1.0 CG1 B:VAL134 4.8 22.4 1.0 CE1 B:PHE418 5.0 24.5 1.0

M.Golczak, P.D.Kiser, D.T.Lodowski, A.Maeda, K.Palczewski. Importance of Membrane Structural Integrity For RPE65 Retinoid Isomerization Activity. J.Biol.Chem. V. 285 9667 2010.
ISSN: ISSN 0021-9258
PubMed: 20100834
DOI: 10.1074/JBC.M109.063941