Iron in PDB 3u8p: Cytochrome B562 Integral Fusion with Egfp

The structure of Cytochrome B562 Integral Fusion with Egfp, PDB code: 3u8p was solved by J.Arpino, H.Czapinska, A.Piasecka, W.R.Edwards, P.Barker, M.Gajda, M.Bochtler, D.D.Jones, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.94 / 2.75
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	64.752, 125.200, 89.255, 90.00, 90.37, 90.00
R / Rfree (%)	20.1 / 24.2

The binding sites of Iron atom in the Cytochrome B562 Integral Fusion with Egfp (pdb code 3u8p). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Cytochrome B562 Integral Fusion with Egfp, PDB code: 3u8p:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Cytochrome B562 Integral Fusion with Egfp


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cytochrome B562 Integral Fusion with Egfp within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe347 b:38.6 occ:1.00 FE A:HEM347 0.0 38.6 1.0 NE2 A:HIS144 1.9 39.1 1.0 NB A:HEM347 2.0 38.5 1.0 ND A:HEM347 2.0 36.2 1.0 NC A:HEM347 2.1 36.2 1.0 NA A:HEM347 2.1 38.8 1.0 SD A:MET49 2.3 47.7 1.0 CD2 A:HIS144 2.9 38.4 1.0 CE1 A:HIS144 3.0 40.6 1.0 C4B A:HEM347 3.0 38.9 1.0 C4D A:HEM347 3.0 37.3 1.0 C1B A:HEM347 3.0 39.5 1.0 C1D A:HEM347 3.0 36.0 1.0 C1C A:HEM347 3.0 36.9 1.0 C4C A:HEM347 3.1 35.7 1.0 C4A A:HEM347 3.1 40.1 1.0 C1A A:HEM347 3.1 39.2 1.0 CG A:MET49 3.3 50.4 1.0 CHC A:HEM347 3.3 37.9 1.0 CE A:MET49 3.4 49.3 1.0 CHB A:HEM347 3.4 40.3 1.0 CHD A:HEM347 3.4 35.6 1.0 CHA A:HEM347 3.4 38.5 1.0 CG A:HIS144 4.1 39.3 1.0 ND1 A:HIS144 4.1 39.7 1.0 C3B A:HEM347 4.2 40.0 1.0 C2B A:HEM347 4.2 40.4 1.0 C2D A:HEM347 4.2 36.3 1.0 C3D A:HEM347 4.2 37.1 1.0 CB A:MET49 4.2 52.4 1.0 C2C A:HEM347 4.2 36.5 1.0 C3C A:HEM347 4.3 35.6 1.0 C3A A:HEM347 4.3 40.9 1.0 C2A A:HEM347 4.3 40.7 1.0 CG A:ARG140 4.8 43.1 1.0

Iron binding site 2 out of 3 in the Cytochrome B562 Integral Fusion with Egfp


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cytochrome B562 Integral Fusion with Egfp within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe347 b:62.4 occ:1.00 FE B:HEM347 0.0 62.4 1.0 NA B:HEM347 2.0 64.2 1.0 NE2 B:HIS144 2.0 55.8 1.0 NB B:HEM347 2.1 63.2 1.0 ND B:HEM347 2.1 62.2 1.0 NC B:HEM347 2.1 62.1 1.0 SD B:MET49 2.4 65.2 1.0 CE1 B:HIS144 2.9 59.2 1.0 C4A B:HEM347 3.0 65.5 1.0 C1B B:HEM347 3.0 64.8 1.0 C1A B:HEM347 3.0 64.7 1.0 C1D B:HEM347 3.1 61.7 1.0 C4D B:HEM347 3.1 62.7 1.0 C4B B:HEM347 3.1 63.0 1.0 C4C B:HEM347 3.1 61.3 1.0 CD2 B:HIS144 3.1 55.6 1.0 C1C B:HEM347 3.1 62.5 1.0 CHB B:HEM347 3.3 65.6 1.0 CHA B:HEM347 3.4 64.0 1.0 CG B:MET49 3.4 65.0 1.0 CHD B:HEM347 3.4 61.4 1.0 CHC B:HEM347 3.4 62.6 1.0 CE B:MET49 3.5 63.6 1.0 ND1 B:HIS144 4.0 57.6 1.0 CB B:MET49 4.1 66.1 1.0 CG B:HIS144 4.2 56.2 1.0 C3A B:HEM347 4.2 66.9 1.0 C2B B:HEM347 4.2 65.6 1.0 C2A B:HEM347 4.2 66.2 1.0 C2D B:HEM347 4.2 62.0 1.0 C3B B:HEM347 4.2 64.6 1.0 C3D B:HEM347 4.3 62.5 1.0 C3C B:HEM347 4.3 62.0 1.0 C2C B:HEM347 4.3 62.1 1.0 CG B:ARG140 4.9 56.6 1.0

Iron binding site 3 out of 3 in the Cytochrome B562 Integral Fusion with Egfp


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cytochrome B562 Integral Fusion with Egfp within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe347 b:51.1 occ:1.00 FE C:HEM347 0.0 51.1 1.0 NE2 C:HIS144 1.9 51.7 1.0 NB C:HEM347 2.0 54.5 1.0 ND C:HEM347 2.0 49.2 1.0 NC C:HEM347 2.1 50.1 1.0 NA C:HEM347 2.1 53.6 1.0 SD C:MET49 2.1 52.6 1.0 CD2 C:HIS144 2.9 51.0 1.0 CE1 C:HIS144 3.0 55.6 1.0 C4B C:HEM347 3.0 55.1 1.0 C1B C:HEM347 3.0 57.0 1.0 C4C C:HEM347 3.0 48.6 1.0 C1D C:HEM347 3.0 47.7 1.0 C1C C:HEM347 3.0 51.4 1.0 C4D C:HEM347 3.1 49.7 1.0 C4A C:HEM347 3.1 56.2 1.0 C1A C:HEM347 3.1 53.6 1.0 CHC C:HEM347 3.3 53.9 1.0 CHB C:HEM347 3.4 57.7 1.0 CE C:MET49 3.4 53.6 1.0 CG C:MET49 3.4 57.3 1.0 CHD C:HEM347 3.4 47.3 1.0 CHA C:HEM347 3.4 51.6 1.0 ND1 C:HIS144 4.1 55.1 1.0 CG C:HIS144 4.1 52.4 1.0 C3B C:HEM347 4.1 58.0 1.0 C2B C:HEM347 4.2 59.2 1.0 C3C C:HEM347 4.2 48.9 1.0 C2D C:HEM347 4.2 47.4 1.0 CB C:MET49 4.2 60.5 1.0 C2C C:HEM347 4.2 50.4 1.0 C3D C:HEM347 4.2 48.4 1.0 C3A C:HEM347 4.3 57.9 1.0 C2A C:HEM347 4.3 56.3 1.0 CG C:ARG140 4.8 57.9 1.0

J.A.Arpino, H.Czapinska, A.Piasecka, W.R.Edwards, P.Barker, M.J.Gajda, M.Bochtler, D.D.Jones. Structural Basis For Efficient Chromophore Communication and Energy Transfer in A Constructed Didomain Protein Scaffold. J.Am.Chem.Soc. V. 134 13632 2012.
ISSN: ISSN 0002-7863
PubMed: 22822710
DOI: 10.1021/JA301987H