Iron in PDB 3wri: Crystal Structure of P450CAM

All present enzymatic activity of Crystal Structure of P450CAM:
1.14.15.1;

The structure of Crystal Structure of P450CAM, PDB code: 3wri was solved by A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, T.Mizushima, H.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.32 / 2.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	61.440, 74.227, 92.636, 90.00, 89.98, 90.00
R / Rfree (%)	19 / 26.8

The binding sites of Iron atom in the Crystal Structure of P450CAM (pdb code 3wri). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of P450CAM, PDB code: 3wri:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:15.0 occ:1.00 FE A:HEM501 0.0 15.0 1.0 NC A:HEM501 1.9 17.2 1.0 NA A:HEM501 2.0 14.4 1.0 NB A:HEM501 2.1 20.6 1.0 ND A:HEM501 2.1 13.8 1.0 SG A:CYS357 2.2 10.1 1.0 C1C A:HEM501 2.9 21.9 1.0 C4C A:HEM501 2.9 15.1 1.0 C4A A:HEM501 3.0 10.1 1.0 C1A A:HEM501 3.1 14.8 1.0 C4B A:HEM501 3.1 15.1 1.0 C1B A:HEM501 3.1 18.2 1.0 C1D A:HEM501 3.1 13.5 1.0 C4D A:HEM501 3.2 13.6 1.0 C5 A:CAM502 3.3 19.4 0.7 CHC A:HEM501 3.4 13.5 1.0 CHD A:HEM501 3.4 17.8 1.0 CHB A:HEM501 3.4 16.8 1.0 CHA A:HEM501 3.6 10.7 1.0 CB A:CYS357 3.7 14.9 1.0 O A:GLY248 4.0 21.0 1.0 C6 A:CAM502 4.1 19.1 0.7 C3C A:HEM501 4.2 15.6 1.0 C2C A:HEM501 4.2 19.6 1.0 C3A A:HEM501 4.3 15.1 1.0 C2A A:HEM501 4.4 13.2 1.0 C3B A:HEM501 4.4 15.9 1.0 C2B A:HEM501 4.5 10.4 1.0 C2D A:HEM501 4.5 13.6 1.0 C3D A:HEM501 4.5 18.8 1.0 CA A:CYS357 4.5 13.9 1.0 C4 A:CAM502 4.6 18.4 0.7 C A:GLY248 4.7 23.0 1.0

Iron binding site 2 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe501 b:15.7 occ:1.00 FE B:HEM501 0.0 15.7 1.0 SG B:CYS357 1.9 31.8 1.0 NC B:HEM501 1.9 24.3 1.0 NA B:HEM501 2.0 16.2 1.0 NB B:HEM501 2.1 18.5 1.0 ND B:HEM501 2.1 16.8 1.0 C1C B:HEM501 2.9 23.0 1.0 C4C B:HEM501 2.9 16.0 1.0 C4A B:HEM501 3.0 10.2 1.0 C1A B:HEM501 3.0 11.9 1.0 C4B B:HEM501 3.1 16.7 1.0 C1D B:HEM501 3.1 15.7 1.0 C1B B:HEM501 3.2 16.6 1.0 C4D B:HEM501 3.2 17.1 1.0 CB B:CYS357 3.2 18.7 1.0 CHC B:HEM501 3.4 14.7 1.0 CHD B:HEM501 3.4 21.0 1.0 CHB B:HEM501 3.5 14.2 1.0 CHA B:HEM501 3.6 11.6 1.0 C5 B:CAM502 3.8 19.4 0.7 CA B:CYS357 4.1 14.3 1.0 C3C B:HEM501 4.1 16.3 1.0 C2C B:HEM501 4.1 21.5 1.0 C3A B:HEM501 4.3 16.1 1.0 C2A B:HEM501 4.3 12.6 1.0 C3B B:HEM501 4.4 18.1 1.0 C2D B:HEM501 4.5 16.4 1.0 C2B B:HEM501 4.5 13.6 1.0 C3D B:HEM501 4.5 20.2 1.0 O B:GLY248 4.6 18.8 1.0 C6 B:CAM502 4.6 20.8 0.7 C B:CYS357 4.7 21.5 1.0 N B:GLY359 4.8 19.2 1.0 N B:LEU358 4.9 20.9 1.0

A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, Y.Katayama, R.Aminaka, M.Ito, T.Mizushima, H.Shimada. Structure of P450CAM Intermediate To Be Published.