Iron in PDB 3wrj: Crystal Structure of P450CAM

All present enzymatic activity of Crystal Structure of P450CAM:
1.14.15.1;

The structure of Crystal Structure of P450CAM, PDB code: 3wrj was solved by A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, T.Mizushima, H.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.92 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	66.885, 62.835, 95.731, 90.00, 90.83, 90.00
R / Rfree (%)	17.3 / 22.1

The structure of Crystal Structure of P450CAM also contains other interesting chemical elements:

Potassium

(K)

2 atoms

The binding sites of Iron atom in the Crystal Structure of P450CAM (pdb code 3wrj). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of P450CAM, PDB code: 3wrj:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:7.5 occ:1.00 FE A:HEM501 0.0 7.5 1.0 NB A:HEM501 2.0 6.0 1.0 NA A:HEM501 2.0 6.3 1.0 ND A:HEM501 2.1 5.1 1.0 NC A:HEM501 2.1 7.8 1.0 SG A:CYS357 2.4 8.4 1.0 C4B A:HEM501 3.0 5.3 1.0 C4A A:HEM501 3.1 4.6 1.0 C1D A:HEM501 3.1 5.3 1.0 C1B A:HEM501 3.1 6.8 1.0 C4C A:HEM501 3.1 7.4 1.0 C4D A:HEM501 3.1 8.0 1.0 C1A A:HEM501 3.1 4.5 1.0 C1C A:HEM501 3.1 9.4 1.0 CB A:CYS357 3.3 7.9 1.0 CHC A:HEM501 3.4 5.8 1.0 CHB A:HEM501 3.5 6.0 1.0 CHD A:HEM501 3.5 7.4 1.0 CHA A:HEM501 3.5 3.1 1.0 CA A:CYS357 4.0 7.0 1.0 C5 A:CAM503 4.1 11.4 1.0 C3B A:HEM501 4.2 5.0 1.0 C2A A:HEM501 4.3 5.2 1.0 C2B A:HEM501 4.3 7.2 1.0 C3A A:HEM501 4.3 3.8 1.0 C3C A:HEM501 4.3 8.4 1.0 C2D A:HEM501 4.3 9.4 1.0 C3D A:HEM501 4.3 4.5 1.0 C2C A:HEM501 4.3 8.0 1.0 N A:GLY359 4.5 8.0 1.0 C4 A:CAM503 4.6 11.4 1.0 C A:CYS357 4.7 9.0 1.0 N A:LEU358 4.7 7.5 1.0 C9 A:CAM503 4.8 5.1 1.0 CG2 A:THR252 4.9 11.2 1.0 CA A:GLY359 4.9 10.0 1.0

Iron binding site 2 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ E:Fe501 b:9.1 occ:1.00 FE E:HEM501 0.0 9.1 1.0 NC E:HEM501 2.1 7.9 1.0 ND E:HEM501 2.1 6.0 1.0 NA E:HEM501 2.1 8.0 1.0 NB E:HEM501 2.1 6.7 1.0 SG E:CYS357 2.4 9.6 1.0 C4D E:HEM501 3.1 8.2 1.0 C1C E:HEM501 3.1 9.5 1.0 C1D E:HEM501 3.1 7.6 1.0 C4B E:HEM501 3.1 8.8 1.0 C4C E:HEM501 3.1 5.8 1.0 C1A E:HEM501 3.1 7.0 1.0 C4A E:HEM501 3.1 7.4 1.0 C1B E:HEM501 3.1 5.1 1.0 CB E:CYS357 3.3 9.8 1.0 CHA E:HEM501 3.4 6.4 1.0 CHC E:HEM501 3.4 9.1 1.0 CHD E:HEM501 3.5 6.9 1.0 CHB E:HEM501 3.5 4.9 1.0 CA E:CYS357 4.0 9.1 1.0 C5 E:CAM503 4.1 13.2 1.0 C2C E:HEM501 4.3 5.5 1.0 C3C E:HEM501 4.3 9.0 1.0 C3B E:HEM501 4.3 5.1 1.0 C2D E:HEM501 4.3 7.0 1.0 C3D E:HEM501 4.3 8.1 1.0 C2A E:HEM501 4.3 8.4 1.0 C3A E:HEM501 4.3 7.9 1.0 C2B E:HEM501 4.3 6.4 1.0 N E:GLY359 4.4 9.1 1.0 C4 E:CAM503 4.5 13.6 1.0 N E:LEU358 4.6 9.7 1.0 C E:CYS357 4.7 10.9 1.0 C9 E:CAM503 4.8 9.1 1.0 CA E:GLY359 4.9 9.2 1.0

A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, Y.Katayama, R.Aminaka, M.Ito, T.Mizushima, H.Shimada. Structure of P450CAM Intermedite To Be Published.