Iron in PDB 3wrm: Crystal Structure of P450CAM

All present enzymatic activity of Crystal Structure of P450CAM:
1.14.15.1;

The structure of Crystal Structure of P450CAM, PDB code: 3wrm was solved by A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, T.Mizushima, H.Shimada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.18 / 1.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	67.086, 62.543, 95.490, 90.00, 90.88, 90.00
R / Rfree (%)	17.7 / 23.3

The structure of Crystal Structure of P450CAM also contains other interesting chemical elements:

Potassium

(K)

2 atoms

The binding sites of Iron atom in the Crystal Structure of P450CAM (pdb code 3wrm). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of P450CAM, PDB code: 3wrm:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:9.8 occ:1.00 FE A:HEM501 0.0 9.8 1.0 NA A:HEM501 2.0 8.2 1.0 NB A:HEM501 2.1 10.3 1.0 NC A:HEM501 2.1 11.0 1.0 ND A:HEM501 2.1 9.9 1.0 SG A:CYS357 2.3 12.2 1.0 C4B A:HEM501 3.1 8.9 1.0 C1B A:HEM501 3.1 9.6 1.0 C1D A:HEM501 3.1 9.1 1.0 C1A A:HEM501 3.1 8.8 1.0 C4A A:HEM501 3.1 6.2 1.0 C4C A:HEM501 3.1 12.8 1.0 C1C A:HEM501 3.1 8.7 1.0 C4D A:HEM501 3.1 11.5 1.0 CB A:CYS357 3.3 10.9 1.0 CHC A:HEM501 3.4 9.7 1.0 CHD A:HEM501 3.5 11.5 1.0 CHB A:HEM501 3.5 8.6 1.0 CHA A:HEM501 3.5 7.0 1.0 CA A:CYS357 4.0 9.9 1.0 C5 A:CAM503 4.2 7.8 1.0 C3B A:HEM501 4.3 5.9 1.0 C2A A:HEM501 4.3 5.6 1.0 C2B A:HEM501 4.3 8.0 1.0 C3A A:HEM501 4.3 7.5 1.0 C2D A:HEM501 4.3 8.0 1.0 C3C A:HEM501 4.3 10.8 1.0 C2C A:HEM501 4.4 8.5 1.0 C3D A:HEM501 4.4 7.6 1.0 N A:GLY359 4.5 14.1 1.0 N A:LEU358 4.6 10.1 1.0 C4 A:CAM503 4.6 10.2 1.0 C A:CYS357 4.7 11.3 1.0 CA A:GLY359 4.9 14.6 1.0 C9 A:CAM503 5.0 6.3 1.0

Iron binding site 2 out of 2 in the Crystal Structure of P450CAM


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of P450CAM within 5.0Å range: probe atom residue distance (Å) B Occ F:Fe501 b:12.2 occ:1.00 FE F:HEM501 0.0 12.2 1.0 NB F:HEM501 2.0 13.4 1.0 NC F:HEM501 2.1 10.4 1.0 ND F:HEM501 2.1 9.4 1.0 NA F:HEM501 2.1 11.4 1.0 SG F:CYS357 2.4 13.1 1.0 C4C F:HEM501 3.0 11.2 1.0 C1B F:HEM501 3.1 11.5 1.0 C1D F:HEM501 3.1 10.5 1.0 C4B F:HEM501 3.1 12.6 1.0 C4A F:HEM501 3.1 11.6 1.0 C1C F:HEM501 3.1 10.8 1.0 C4D F:HEM501 3.1 9.9 1.0 C1A F:HEM501 3.1 12.0 1.0 CB F:CYS357 3.4 11.2 1.0 CHD F:HEM501 3.4 8.9 1.0 CHB F:HEM501 3.4 9.8 1.0 CHC F:HEM501 3.5 12.8 1.0 CHA F:HEM501 3.5 11.4 1.0 CA F:CYS357 4.0 12.4 1.0 C5 F:CAM503 4.0 10.5 1.0 C3C F:HEM501 4.3 9.7 1.0 C2B F:HEM501 4.3 10.2 1.0 C2C F:HEM501 4.3 10.7 1.0 C3B F:HEM501 4.3 9.9 1.0 C2D F:HEM501 4.3 9.8 1.0 C3A F:HEM501 4.3 9.6 1.0 C3D F:HEM501 4.3 10.4 1.0 C2A F:HEM501 4.4 10.9 1.0 N F:GLY359 4.5 15.3 1.0 C4 F:CAM503 4.5 11.4 1.0 N F:LEU358 4.6 15.0 1.0 C F:CYS357 4.7 13.8 1.0 C9 F:CAM503 4.8 9.5 1.0 CA F:GLY359 4.9 13.2 1.0

A.Kishimoto, K.Takagi, A.Amano, K.Sakurai, Y.Katayama, R.Aminaka, M.Ito, T.Mizushima, H.Shimada. Structure of P450CAM Intermedite To Be Published.