Iron in PDB 3zh0: Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin

The structure of Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin, PDB code: 3zh0 was solved by C.Ciaccio, A.Pesce, G.R.Tundo, L.Tilleman, S.Dewilde, L.Moens, P.Ascenzi, M.Bolognesi, M.Nardini, M.Coletta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	91.93 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	80.670, 48.620, 92.030, 90.00, 92.61, 90.00
R / Rfree (%)	19.919 / 26.015

The binding sites of Iron atom in the Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin (pdb code 3zh0). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin, PDB code: 3zh0:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe200 b:7.8 occ:1.00 FE A:HEM200 0.0 7.8 1.0 NA A:HEM200 2.0 4.5 1.0 NC A:HEM200 2.0 8.9 1.0 NB A:HEM200 2.0 8.7 1.0 NE2 A:HIS120 2.0 7.6 1.0 O1 A:FMT201 2.1 15.0 1.0 ND A:HEM200 2.1 8.5 1.0 C A:FMT201 2.9 15.8 1.0 C4C A:HEM200 3.0 8.3 1.0 C4A A:HEM200 3.0 6.0 1.0 C1C A:HEM200 3.0 7.5 1.0 C1A A:HEM200 3.0 5.4 1.0 CE1 A:HIS120 3.0 7.8 1.0 CD2 A:HIS120 3.0 6.7 1.0 C1B A:HEM200 3.0 6.3 1.0 C4B A:HEM200 3.0 9.2 1.0 C4D A:HEM200 3.0 8.2 1.0 C1D A:HEM200 3.1 8.5 1.0 CHD A:HEM200 3.3 6.7 1.0 CHB A:HEM200 3.3 6.7 1.0 CHC A:HEM200 3.4 7.9 1.0 CHA A:HEM200 3.4 6.4 1.0 O2 A:FMT201 4.1 17.4 1.0 ND1 A:HIS120 4.1 7.7 1.0 CG A:HIS120 4.2 5.6 1.0 C2A A:HEM200 4.2 5.1 1.0 C2C A:HEM200 4.2 8.3 1.0 C3A A:HEM200 4.2 6.2 1.0 C3C A:HEM200 4.2 7.3 1.0 C3D A:HEM200 4.3 9.0 1.0 C2B A:HEM200 4.3 6.9 1.0 C3B A:HEM200 4.3 7.8 1.0 C2D A:HEM200 4.3 7.8 1.0

Iron binding site 2 out of 4 in the Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe200 b:7.9 occ:1.00 FE B:HEM200 0.0 7.9 1.0 NA B:HEM200 2.0 7.5 1.0 NC B:HEM200 2.0 10.3 1.0 O1 B:FMT201 2.0 10.2 1.0 ND B:HEM200 2.0 7.8 1.0 NB B:HEM200 2.1 10.5 1.0 NE2 B:HIS120 2.1 9.6 1.0 C B:FMT201 2.9 11.1 1.0 C4A B:HEM200 3.0 9.6 1.0 C1A B:HEM200 3.0 9.2 1.0 C4C B:HEM200 3.0 9.3 1.0 C1D B:HEM200 3.0 6.1 1.0 C4D B:HEM200 3.0 7.4 1.0 C1C B:HEM200 3.0 8.9 1.0 CD2 B:HIS120 3.0 8.1 1.0 C4B B:HEM200 3.0 11.9 1.0 C1B B:HEM200 3.1 9.0 1.0 CE1 B:HIS120 3.1 9.5 1.0 CHD B:HEM200 3.3 6.8 1.0 CHC B:HEM200 3.3 10.6 1.0 CHA B:HEM200 3.3 6.1 1.0 CHB B:HEM200 3.4 9.1 1.0 O2 B:FMT201 4.1 12.8 1.0 C3A B:HEM200 4.2 8.5 1.0 C2A B:HEM200 4.2 8.2 1.0 CG B:HIS120 4.2 9.7 1.0 C3C B:HEM200 4.2 8.0 1.0 C2C B:HEM200 4.2 7.5 1.0 C3D B:HEM200 4.2 6.4 1.0 ND1 B:HIS120 4.2 10.3 1.0 C2D B:HEM200 4.2 6.9 1.0 C3B B:HEM200 4.3 10.4 1.0 C2B B:HEM200 4.3 10.2 1.0

Iron binding site 3 out of 4 in the Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe200 b:8.8 occ:1.00 FE C:HEM200 0.0 8.8 1.0 NA C:HEM200 2.0 8.4 1.0 NC C:HEM200 2.0 5.9 1.0 NB C:HEM200 2.0 7.0 1.0 O1 C:FMT201 2.0 12.2 1.0 NE2 C:HIS120 2.1 8.7 1.0 ND C:HEM200 2.1 7.1 1.0 C C:FMT201 2.9 12.1 1.0 C4A C:HEM200 2.9 8.3 1.0 CE1 C:HIS120 3.0 8.0 1.0 C1B C:HEM200 3.0 7.8 1.0 C1A C:HEM200 3.0 8.6 1.0 C4C C:HEM200 3.0 7.4 1.0 C1D C:HEM200 3.0 7.8 1.0 C1C C:HEM200 3.0 6.1 1.0 C4B C:HEM200 3.1 9.3 1.0 C4D C:HEM200 3.1 9.6 1.0 CD2 C:HIS120 3.1 8.7 1.0 CHB C:HEM200 3.3 6.1 1.0 CHD C:HEM200 3.3 6.3 1.0 CHC C:HEM200 3.4 5.7 1.0 CHA C:HEM200 3.4 8.7 1.0 O2 C:FMT201 4.0 13.5 1.0 ND1 C:HIS120 4.1 8.9 1.0 C3A C:HEM200 4.1 7.1 1.0 C2A C:HEM200 4.2 8.4 1.0 C2B C:HEM200 4.2 7.9 1.0 CG C:HIS120 4.2 8.6 1.0 C2D C:HEM200 4.3 6.8 1.0 C2C C:HEM200 4.3 7.0 1.0 C3C C:HEM200 4.3 4.5 1.0 C3B C:HEM200 4.3 6.5 1.0 C3D C:HEM200 4.3 7.6 1.0

Iron binding site 4 out of 4 in the Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe200 b:7.2 occ:1.00 FE D:HEM200 0.0 7.2 1.0 NA D:HEM200 2.0 7.6 1.0 NB D:HEM200 2.0 7.2 1.0 NC D:HEM200 2.0 6.8 1.0 ND D:HEM200 2.0 5.9 1.0 O1 D:FMT201 2.0 9.1 1.0 NE2 D:HIS120 2.1 7.2 1.0 C D:FMT201 2.9 9.8 1.0 C4A D:HEM200 3.0 8.8 1.0 C1B D:HEM200 3.0 5.7 1.0 C1D D:HEM200 3.0 5.0 1.0 CD2 D:HIS120 3.0 6.2 1.0 C1A D:HEM200 3.0 9.3 1.0 C4D D:HEM200 3.0 8.4 1.0 C4C D:HEM200 3.0 7.1 1.0 C4B D:HEM200 3.0 7.9 1.0 C1C D:HEM200 3.1 5.7 1.0 CE1 D:HIS120 3.1 4.4 1.0 CHB D:HEM200 3.3 6.0 1.0 CHD D:HEM200 3.3 5.3 1.0 CHA D:HEM200 3.4 9.7 1.0 CHC D:HEM200 3.4 6.9 1.0 O2 D:FMT201 4.1 9.4 1.0 CG D:HIS120 4.2 8.5 1.0 ND1 D:HIS120 4.2 7.1 1.0 C3A D:HEM200 4.2 11.2 1.0 C2A D:HEM200 4.2 7.1 1.0 C2B D:HEM200 4.2 6.7 1.0 C2D D:HEM200 4.2 6.5 1.0 C3D D:HEM200 4.2 6.2 1.0 C3C D:HEM200 4.2 2.9 1.0 C3B D:HEM200 4.3 6.1 1.0 C2C D:HEM200 4.3 5.0 1.0

C.Ciaccio, A.Pesce, G.R.Tundo, L.Tilleman, L.Bertolacci, S.Dewilde, L.Moens, P.Ascenzi, M.Bolognesi, M.Nardini, M.Coletta. Functional and Structural Role of the N-Terminal Extension in Methanosarcina Acetivorans Protoglobin. Biochim.Biophys.Acta V.1834 1813 2013.
ISSN: ISSN 0006-3002
PubMed: 23485914
DOI: 10.1016/J.BBAPAP.2013.02.026