Iron in PDB 3zsn: Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV

The structure of Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV, PDB code: 3zsn was solved by S.Li, P.M.Kells, F.U.Rutaganira, Y.Anzai, F.Kato, D.H.Sherman, L.M.Podust, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	220.42 / 1.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	58.214, 100.940, 440.842, 90.00, 90.00, 90.00
R / Rfree (%)	18.522 / 24.497

The binding sites of Iron atom in the Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV (pdb code 3zsn). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV, PDB code: 3zsn:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe450 b:14.8 occ:1.00 FE A:HEM450 0.0 14.8 1.0 NA A:HEM450 2.0 10.1 1.0 NC A:HEM450 2.0 10.2 1.0 NB A:HEM450 2.0 12.5 1.0 ND A:HEM450 2.1 11.4 1.0 SG A:CYS346 2.4 13.6 1.0 O A:HOH2221 2.4 20.9 1.0 C1A A:HEM450 3.0 13.9 1.0 C4C A:HEM450 3.0 13.9 1.0 C1C A:HEM450 3.0 12.5 1.0 C4A A:HEM450 3.0 11.8 1.0 C4B A:HEM450 3.0 9.1 1.0 C4D A:HEM450 3.1 11.7 1.0 C1B A:HEM450 3.1 11.6 1.0 C1D A:HEM450 3.1 12.0 1.0 CHC A:HEM450 3.4 11.2 1.0 CB A:CYS346 3.4 9.4 1.0 CHA A:HEM450 3.4 10.9 1.0 CHD A:HEM450 3.4 13.0 1.0 CHB A:HEM450 3.5 10.9 1.0 CA A:CYS346 4.0 11.1 1.0 C3C A:HEM450 4.2 14.1 1.0 C2A A:HEM450 4.2 10.7 1.0 O A:ALA234 4.2 19.7 1.0 C2C A:HEM450 4.2 12.0 1.0 C3A A:HEM450 4.2 9.7 1.0 C3B A:HEM450 4.3 12.8 1.0 C2B A:HEM450 4.3 11.3 1.0 C3D A:HEM450 4.4 11.8 1.0 C2D A:HEM450 4.4 14.4 1.0 O A:HOH2223 4.4 51.8 1.0 N A:GLY348 4.8 15.6 1.0 C A:CYS346 4.8 14.6 1.0 C32 A:MIV460 4.8 40.0 1.0 N A:LEU347 4.9 15.6 1.0 CB A:ALA234 5.0 17.1 1.0 C A:ALA234 5.0 18.3 1.0

Iron binding site 2 out of 3 in the Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe450 b:13.9 occ:1.00 FE B:HEM450 0.0 13.9 1.0 NC B:HEM450 2.0 11.9 1.0 NA B:HEM450 2.0 9.6 1.0 NB B:HEM450 2.0 11.7 1.0 ND B:HEM450 2.1 10.7 1.0 SG B:CYS346 2.4 13.6 1.0 O B:HOH2235 2.5 19.4 1.0 C4C B:HEM450 3.0 12.9 1.0 C1C B:HEM450 3.0 12.9 1.0 C4B B:HEM450 3.0 10.5 1.0 C4A B:HEM450 3.0 12.0 1.0 C1A B:HEM450 3.1 13.1 1.0 C1D B:HEM450 3.1 9.2 1.0 C4D B:HEM450 3.1 11.2 1.0 C1B B:HEM450 3.1 9.7 1.0 CHD B:HEM450 3.4 10.1 1.0 CB B:CYS346 3.4 9.3 1.0 CHC B:HEM450 3.4 10.4 1.0 CHA B:HEM450 3.4 12.8 1.0 CHB B:HEM450 3.4 10.4 1.0 CA B:CYS346 4.1 10.9 1.0 C3C B:HEM450 4.2 15.1 1.0 C2C B:HEM450 4.2 12.1 1.0 C3B B:HEM450 4.3 12.8 1.0 C3A B:HEM450 4.3 10.4 1.0 C2A B:HEM450 4.3 10.7 1.0 O B:ALA234 4.3 19.2 1.0 C2B B:HEM450 4.3 12.6 1.0 C3D B:HEM450 4.3 11.6 1.0 C2D B:HEM450 4.3 13.0 1.0 C B:CYS346 4.8 13.8 1.0 N B:GLY348 4.8 15.1 1.0 CB B:ALA234 4.8 16.9 1.0 N B:LEU347 4.9 14.4 0.5 N B:LEU347 4.9 14.4 0.5

Iron binding site 3 out of 3 in the Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Structure of the Mixed-Function P450 Mycg F286A Mutant in Complex with Mycinamicin IV within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe450 b:14.4 occ:1.00 FE C:HEM450 0.0 14.4 1.0 NC C:HEM450 2.0 9.8 1.0 NB C:HEM450 2.0 12.7 1.0 NA C:HEM450 2.1 10.8 1.0 ND C:HEM450 2.1 12.2 1.0 SG C:CYS346 2.4 14.0 1.0 O C:HOH2235 2.6 22.7 1.0 C4C C:HEM450 3.0 11.8 1.0 C1C C:HEM450 3.0 13.2 1.0 C4B C:HEM450 3.0 9.1 1.0 C4A C:HEM450 3.0 12.3 1.0 C1A C:HEM450 3.1 13.6 1.0 C4D C:HEM450 3.1 12.8 1.0 C1B C:HEM450 3.1 11.3 1.0 C1D C:HEM450 3.1 10.8 1.0 CB C:CYS346 3.3 10.8 1.0 CHA C:HEM450 3.4 13.2 1.0 CHC C:HEM450 3.4 12.7 1.0 CHD C:HEM450 3.4 10.5 1.0 CHB C:HEM450 3.4 10.7 1.0 CA C:CYS346 4.0 12.6 1.0 C3C C:HEM450 4.2 11.8 1.0 C2C C:HEM450 4.3 11.1 1.0 C3B C:HEM450 4.3 12.8 1.0 C3A C:HEM450 4.3 11.1 1.0 C2A C:HEM450 4.3 7.7 1.0 O C:ALA234 4.3 18.4 1.0 C3D C:HEM450 4.3 13.1 1.0 C2B C:HEM450 4.3 11.6 1.0 C2D C:HEM450 4.3 13.1 1.0 O C:HOH2238 4.7 38.1 1.0 C C:CYS346 4.7 14.7 1.0 N C:GLY348 4.8 14.8 1.0 C32 C:MIV460 4.8 37.6 1.0 N C:LEU347 4.8 15.0 0.5 CB C:ALA234 4.9 17.2 1.0 N C:LEU347 4.9 15.1 0.5

S.Li, D.R.Tietz, F.U.Rutaganira, P.M.Kells, Y.Anzai, F.Kato, T.C.Pochapsky, D.H.Sherman, L.M.Podust. Substrate Recognition By the Multifunctional Cytochrome P450 Mycg in Mycinamicin Hydroxylation and Epoxidation Reactions. J.Biol.Chem. V. 287 37880 2012.
ISSN: ISSN 0021-9258
PubMed: 22952225
DOI: 10.1074/JBC.M112.410340