Iron in PDB 4ayb: Rnap at 3.2ANG
Enzymatic activity of Rnap at 3.2ANG
All present enzymatic activity of Rnap at 3.2ANG:
2.7.7.6;
Protein crystallography data
The structure of Rnap at 3.2ANG, PDB code: 4ayb
was solved by
M.N.Wojtas,
M.Mogni,
O.Millet,
S.D.Bell,
N.G.A.Abrescia,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
40.10 /
3.20
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
195.725,
212.415,
128.757,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
24.3 /
30.1
|
Other elements in 4ayb:
The structure of Rnap at 3.2ANG also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Rnap at 3.2ANG
(pdb code 4ayb). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the
Rnap at 3.2ANG, PDB code: 4ayb:
Jump to Iron binding site number:
1;
2;
3;
Iron binding site 1 out
of 3 in 4ayb
Go back to
Iron Binding Sites List in 4ayb
Iron binding site 1 out
of 3 in the Rnap at 3.2ANG
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Rnap at 3.2ANG within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Fe1264
b:81.4
occ:1.00
|
FE1
|
D:SF41264
|
0.0
|
81.4
|
1.0
|
SG
|
D:CYS209
|
2.3
|
69.3
|
1.0
|
S3
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
S4
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
S2
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
FE3
|
D:SF41264
|
2.7
|
81.4
|
1.0
|
FE2
|
D:SF41264
|
2.7
|
81.4
|
1.0
|
H
|
D:CYS209
|
3.1
|
81.4
|
1.0
|
HB3
|
D:CYS209
|
3.2
|
81.4
|
1.0
|
CB
|
D:CYS209
|
3.4
|
71.5
|
1.0
|
HE2
|
D:PHE188
|
3.6
|
81.4
|
1.0
|
SG
|
D:CYS183
|
3.8
|
77.8
|
1.0
|
S1
|
D:SF41264
|
3.9
|
81.4
|
1.0
|
HB2
|
D:CYS183
|
3.9
|
81.4
|
1.0
|
N
|
D:CYS209
|
3.9
|
80.9
|
1.0
|
HG
|
D:CYS183
|
3.9
|
81.4
|
1.0
|
SG
|
D:CYS206
|
3.9
|
0.6
|
1.0
|
H
|
D:GLU207
|
4.0
|
81.4
|
1.0
|
HG12
|
D:ILE219
|
4.0
|
81.4
|
1.0
|
H
|
D:GLU208
|
4.0
|
81.4
|
1.0
|
HB2
|
D:CYS209
|
4.1
|
81.4
|
1.0
|
HB3
|
D:CYS206
|
4.1
|
81.4
|
1.0
|
CA
|
D:CYS209
|
4.3
|
83.5
|
1.0
|
CE2
|
D:PHE188
|
4.3
|
87.4
|
1.0
|
HD12
|
D:ILE219
|
4.3
|
81.4
|
1.0
|
CB
|
D:CYS183
|
4.4
|
79.7
|
1.0
|
HB2
|
D:GLU208
|
4.4
|
81.4
|
1.0
|
HA
|
D:CYS183
|
4.4
|
81.4
|
1.0
|
HA
|
D:GLU207
|
4.4
|
81.4
|
1.0
|
N
|
D:GLU208
|
4.5
|
96.1
|
1.0
|
HZ
|
D:PHE188
|
4.6
|
81.4
|
1.0
|
N
|
D:GLU207
|
4.6
|
88.1
|
1.0
|
SG
|
D:CYS203
|
4.6
|
62.5
|
1.0
|
CB
|
D:CYS206
|
4.7
|
99.8
|
1.0
|
HD2
|
D:PRO184
|
4.7
|
81.4
|
1.0
|
CG1
|
D:ILE219
|
4.7
|
74.6
|
1.0
|
HG13
|
D:ILE219
|
4.8
|
81.4
|
1.0
|
CZ
|
D:PHE188
|
4.8
|
88.0
|
1.0
|
H
|
D:LEU210
|
4.8
|
81.4
|
1.0
|
HA
|
D:CYS209
|
4.9
|
81.4
|
1.0
|
CA
|
D:GLU207
|
4.9
|
89.0
|
1.0
|
CA
|
D:CYS183
|
5.0
|
66.8
|
1.0
|
CD1
|
D:ILE219
|
5.0
|
76.3
|
1.0
|
HB
|
D:VAL187
|
5.0
|
81.4
|
1.0
|
|
Iron binding site 2 out
of 3 in 4ayb
Go back to
Iron Binding Sites List in 4ayb
Iron binding site 2 out
of 3 in the Rnap at 3.2ANG
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Rnap at 3.2ANG within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Fe1264
b:81.4
occ:1.00
|
FE2
|
D:SF41264
|
0.0
|
81.4
|
1.0
|
S4
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
S3
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
S1
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
SG
|
D:CYS203
|
2.3
|
62.5
|
1.0
|
HG22
|
D:VAL187
|
2.7
|
81.4
|
1.0
|
FE3
|
D:SF41264
|
2.7
|
81.4
|
1.0
|
FE1
|
D:SF41264
|
2.7
|
81.4
|
1.0
|
HB2
|
D:CYS203
|
3.1
|
81.4
|
1.0
|
CB
|
D:CYS203
|
3.3
|
63.7
|
1.0
|
HG
|
D:CYS183
|
3.4
|
81.4
|
1.0
|
HA
|
D:CYS203
|
3.4
|
81.4
|
1.0
|
CG2
|
D:VAL187
|
3.5
|
72.1
|
1.0
|
HB
|
D:VAL187
|
3.7
|
81.4
|
1.0
|
HG21
|
D:VAL187
|
3.7
|
81.4
|
1.0
|
HG12
|
D:VAL187
|
3.8
|
81.4
|
1.0
|
SG
|
D:CYS183
|
3.8
|
77.8
|
1.0
|
S2
|
D:SF41264
|
3.9
|
81.4
|
1.0
|
H
|
D:THR204
|
3.9
|
81.4
|
1.0
|
CA
|
D:CYS203
|
3.9
|
72.9
|
1.0
|
CB
|
D:VAL187
|
4.1
|
74.8
|
1.0
|
HB3
|
D:CYS203
|
4.1
|
81.4
|
1.0
|
HG22
|
D:VAL170
|
4.2
|
81.4
|
1.0
|
HG23
|
D:VAL187
|
4.2
|
81.4
|
1.0
|
HG12
|
D:ILE219
|
4.3
|
81.4
|
1.0
|
HB3
|
D:CYS206
|
4.3
|
81.4
|
1.0
|
HG23
|
D:VAL170
|
4.3
|
81.4
|
1.0
|
HG13
|
D:ILE219
|
4.4
|
81.4
|
1.0
|
CG1
|
D:VAL187
|
4.4
|
76.1
|
1.0
|
SG
|
D:CYS209
|
4.5
|
69.3
|
1.0
|
N
|
D:THR204
|
4.6
|
80.4
|
1.0
|
CG2
|
D:VAL170
|
4.7
|
0.1
|
1.0
|
HE2
|
D:PHE188
|
4.8
|
81.4
|
1.0
|
CG1
|
D:ILE219
|
4.8
|
74.6
|
1.0
|
C
|
D:CYS203
|
4.8
|
70.5
|
1.0
|
HB2
|
D:GLU185
|
4.9
|
81.4
|
1.0
|
H
|
D:GLU207
|
5.0
|
81.4
|
1.0
|
SG
|
D:CYS206
|
5.0
|
0.6
|
1.0
|
CB
|
D:CYS206
|
5.0
|
99.8
|
1.0
|
N
|
D:CYS203
|
5.0
|
74.3
|
1.0
|
HZ
|
D:PHE188
|
5.0
|
81.4
|
1.0
|
|
Iron binding site 3 out
of 3 in 4ayb
Go back to
Iron Binding Sites List in 4ayb
Iron binding site 3 out
of 3 in the Rnap at 3.2ANG
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Rnap at 3.2ANG within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Fe1264
b:81.4
occ:1.00
|
FE3
|
D:SF41264
|
0.0
|
81.4
|
1.0
|
HB3
|
D:CYS206
|
2.1
|
81.4
|
1.0
|
S4
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
S1
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
S2
|
D:SF41264
|
2.3
|
81.4
|
1.0
|
SG
|
D:CYS206
|
2.3
|
0.6
|
1.0
|
CB
|
D:CYS206
|
2.5
|
99.8
|
1.0
|
FE2
|
D:SF41264
|
2.7
|
81.4
|
1.0
|
FE1
|
D:SF41264
|
2.7
|
81.4
|
1.0
|
HB2
|
D:CYS206
|
2.7
|
81.4
|
1.0
|
H
|
D:GLU207
|
3.3
|
81.4
|
1.0
|
HD2
|
D:PRO184
|
3.7
|
81.4
|
1.0
|
H
|
D:THR204
|
3.7
|
81.4
|
1.0
|
S3
|
D:SF41264
|
3.9
|
81.4
|
1.0
|
SG
|
D:CYS183
|
3.9
|
77.8
|
1.0
|
SG
|
D:CYS203
|
3.9
|
62.5
|
1.0
|
CA
|
D:CYS206
|
4.0
|
90.6
|
1.0
|
N
|
D:GLU207
|
4.0
|
88.1
|
1.0
|
H
|
D:GLU208
|
4.1
|
81.4
|
1.0
|
HA
|
D:CYS203
|
4.2
|
81.4
|
1.0
|
HG
|
D:CYS183
|
4.3
|
81.4
|
1.0
|
O
|
D:LEU205
|
4.4
|
97.0
|
1.0
|
C
|
D:CYS206
|
4.5
|
91.2
|
1.0
|
HG2
|
D:GLU185
|
4.5
|
81.4
|
1.0
|
N
|
D:THR204
|
4.5
|
80.4
|
1.0
|
HA
|
D:CYS206
|
4.5
|
81.4
|
1.0
|
CD
|
D:PRO184
|
4.6
|
69.8
|
1.0
|
OG1
|
D:THR204
|
4.6
|
77.8
|
1.0
|
HA
|
D:CYS183
|
4.6
|
81.4
|
1.0
|
HB2
|
D:GLU208
|
4.6
|
81.4
|
1.0
|
HG22
|
D:VAL187
|
4.7
|
81.4
|
1.0
|
HG2
|
D:PRO184
|
4.7
|
81.4
|
1.0
|
O
|
D:THR204
|
4.7
|
78.9
|
1.0
|
N
|
D:CYS206
|
4.7
|
89.5
|
1.0
|
SG
|
D:CYS209
|
4.7
|
69.3
|
1.0
|
H
|
D:CYS209
|
4.8
|
81.4
|
1.0
|
C
|
D:LEU205
|
4.8
|
95.4
|
1.0
|
HG3
|
D:GLU185
|
4.8
|
81.4
|
1.0
|
HD3
|
D:PRO184
|
4.9
|
81.4
|
1.0
|
N
|
D:GLU208
|
4.9
|
96.1
|
1.0
|
HB2
|
D:GLU185
|
4.9
|
81.4
|
1.0
|
HA
|
D:GLU207
|
5.0
|
81.4
|
1.0
|
|
Reference:
M.N.Wojtas,
M.Mogni,
O.Millet,
S.D.Bell,
N.G.A.Abrescia.
Structural and Functional Analyses of the Interaction of Archaeal Rna Polymerase with Dna. Nucleic Acids Res. V. 40 9941 2012.
ISSN: ISSN 0305-1048
PubMed: 22848102
DOI: 10.1093/NAR/GKS692
Page generated: Sun Aug 4 23:48:36 2024
|