Iron in PDB 4ceh: Crystal Structure of Addab with A Forked Dna Substrate

All present enzymatic activity of Crystal Structure of Addab with A Forked Dna Substrate:
3.6.4.12;

The structure of Crystal Structure of Addab with A Forked Dna Substrate, PDB code: 4ceh was solved by W.W.Krajewski, M.Wilkinson, X.Fu, N.B.Cronin, D.Wigley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.939 / 3.24
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	77.373, 96.766, 109.693, 104.38, 96.11, 90.03
R / Rfree (%)	22.92 / 26.38

The binding sites of Iron atom in the Crystal Structure of Addab with A Forked Dna Substrate (pdb code 4ceh). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Crystal Structure of Addab with A Forked Dna Substrate, PDB code: 4ceh:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Crystal Structure of Addab with A Forked Dna Substrate


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Addab with A Forked Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2161 b:0.9 occ:1.00 FE1 B:SF42161 0.0 0.9 1.0 S2 B:SF42161 2.2 0.5 1.0 S3 B:SF42161 2.2 90.0 1.0 S4 B:SF42161 2.2 0.0 1.0 SG B:CYS1124 2.3 0.7 1.0 CB B:CYS1124 3.0 92.6 1.0 FE3 B:SF42161 3.1 0.2 1.0 FE4 B:SF42161 3.1 0.4 1.0 FE2 B:SF42161 3.1 81.7 1.0 S1 B:SF42161 3.8 79.7 1.0 CB B:SER804 4.1 0.5 1.0 CB B:PHE1126 4.4 82.3 1.0 CA B:CYS1124 4.5 96.9 1.0 CA B:CYS1121 4.7 0.6 1.0 C B:PHE1126 4.7 86.8 1.0 SG B:CYS801 4.8 0.7 1.0 CA B:SER804 4.8 99.9 1.0 N B:PHE1126 4.8 99.4 1.0 O B:PHE1126 4.9 91.1 1.0 CA B:PHE1126 4.9 83.3 1.0 C B:CYS1124 5.0 1.0 1.0 N B:LYS1127 5.0 93.0 1.0

Iron binding site 2 out of 4 in the Crystal Structure of Addab with A Forked Dna Substrate


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Addab with A Forked Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2161 b:81.7 occ:1.00 FE2 B:SF42161 0.0 81.7 1.0 S1 B:SF42161 2.2 79.7 1.0 S3 B:SF42161 2.2 90.0 1.0 S4 B:SF42161 2.2 0.0 1.0 SG B:CYS801 2.3 0.7 1.0 FE4 B:SF42161 3.1 0.4 1.0 FE3 B:SF42161 3.1 0.2 1.0 FE1 B:SF42161 3.1 0.9 1.0 CB B:CYS801 3.4 0.8 1.0 S2 B:SF42161 3.8 0.5 1.0 CG2 B:ILE1110 4.3 0.2 1.0 CG B:PRO1112 4.3 0.8 1.0 CB B:SER804 4.4 0.5 1.0 N B:SER804 4.4 0.7 1.0 CB B:PRO1112 4.5 0.3 1.0 CB B:PHE803 4.8 99.8 1.0 CA B:CYS801 4.8 0.8 1.0 CA B:SER804 4.9 99.9 1.0 OG B:SER804 4.9 0.5 1.0 N B:PRO1112 5.0 0.5 1.0 CA B:PRO1112 5.0 0.5 1.0

Iron binding site 3 out of 4 in the Crystal Structure of Addab with A Forked Dna Substrate


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Crystal Structure of Addab with A Forked Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2161 b:0.2 occ:1.00 FE3 B:SF42161 0.0 0.2 1.0 S2 B:SF42161 2.2 0.5 1.0 S1 B:SF42161 2.2 79.7 1.0 S4 B:SF42161 2.2 0.0 1.0 SG B:CYS1130 2.3 0.8 1.0 CB B:CYS1130 3.1 88.8 1.0 FE4 B:SF42161 3.1 0.4 1.0 FE1 B:SF42161 3.1 0.9 1.0 FE2 B:SF42161 3.1 81.7 1.0 S3 B:SF42161 3.8 90.0 1.0 O B:PHE1126 3.9 91.1 1.0 CA B:LYS1127 4.3 93.0 1.0 C B:PHE1126 4.4 86.8 1.0 CA B:CYS1130 4.5 0.8 1.0 CB B:PHE1132 4.5 95.8 1.0 N B:LYS1127 4.7 93.0 1.0 CG2 B:ILE1110 4.8 0.2 1.0 N B:CYS1130 4.9 0.7 1.0

Iron binding site 4 out of 4 in the Crystal Structure of Addab with A Forked Dna Substrate


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Crystal Structure of Addab with A Forked Dna Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe2161 b:0.4 occ:1.00 FE4 B:SF42161 0.0 0.4 1.0 S1 B:SF42161 2.2 79.7 1.0 S2 B:SF42161 2.2 0.5 1.0 S3 B:SF42161 2.2 90.0 1.0 SG B:CYS1121 2.3 0.7 1.0 CB B:CYS1121 2.9 98.8 1.0 FE3 B:SF42161 3.1 0.2 1.0 FE1 B:SF42161 3.1 0.9 1.0 FE2 B:SF42161 3.1 81.7 1.0 CA B:CYS1121 3.2 0.6 1.0 S4 B:SF42161 3.8 0.0 1.0 N B:CYS1121 3.8 95.9 1.0 CB B:PRO1112 4.2 0.3 1.0 CG B:PRO1112 4.4 0.8 1.0 C B:CYS1121 4.6 99.2 1.0 C B:PRO1120 4.6 97.8 1.0 CB B:CYS1124 4.8 92.6 1.0 O B:CYS1121 4.9 0.6 1.0

W.W.Krajewski, X.Fu, M.Wilkinson, N.B.Cronin, M.S.Dillingham, D.B.Wigley. Structural Basis For Translocation By Addab Helicase-Nuclease and Its Arrest at Chi Sites. Nature V. 508 416 2014.
ISSN: ISSN 0028-0836
PubMed: 24670664
DOI: 10.1038/NATURE13037