Iron in PDB 4eb7: A. Fulgidus Iscs-Iscu Complex Structure

All present enzymatic activity of A. Fulgidus Iscs-Iscu Complex Structure:
2.8.1.7;

The structure of A. Fulgidus Iscs-Iscu Complex Structure, PDB code: 4eb7 was solved by E.N.Marinoni, J.S.De Oliveira, Y.Nicolet, E.C.Raulfs, P.Amara, D.R.Dean, J.C.Fontecilla-Camps, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	81.37 / 2.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	75.469, 96.129, 152.793, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 27.3

The binding sites of Iron atom in the A. Fulgidus Iscs-Iscu Complex Structure (pdb code 4eb7). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the A. Fulgidus Iscs-Iscu Complex Structure, PDB code: 4eb7:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the A. Fulgidus Iscs-Iscu Complex Structure


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of A. Fulgidus Iscs-Iscu Complex Structure within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe201 b:62.6 occ:0.60 FE1 C:FES201 0.0 62.6 0.6 SG C:CYS102 2.2 80.6 1.0 SG C:CYS58 2.2 72.9 1.0 S1 C:FES201 2.4 64.1 0.6 S2 C:FES202 2.8 64.3 0.6 CB C:CYS58 3.0 67.7 1.0 FE2 C:FES201 3.6 70.6 0.6 N C:CYS58 3.9 63.4 1.0 NE2 C:HIS101 4.0 98.4 1.0 CB C:CYS102 4.0 76.7 1.0 CA C:CYS58 4.0 64.6 1.0 SG A:CYS321 4.2 73.2 1.0 CE1 C:HIS101 4.4 99.2 1.0 N C:ALA61 4.6 58.6 1.0 C C:CYS58 4.8 61.8 1.0 CB C:ALA61 4.8 56.8 1.0 CD2 C:HIS101 4.9 94.7 1.0 CB A:CYS321 5.0 67.9 1.0

Iron binding site 2 out of 2 in the A. Fulgidus Iscs-Iscu Complex Structure


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of A. Fulgidus Iscs-Iscu Complex Structure within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe201 b:70.6 occ:0.60 FE2 C:FES201 0.0 70.6 0.6 S1 C:FES201 2.4 64.1 0.6 S2 C:FES202 2.6 64.3 0.6 NE2 C:HIS101 2.7 98.4 1.0 CB C:CYS33 3.2 92.1 1.0 CB C:ASP35 3.3 69.8 1.0 FE1 C:FES201 3.6 62.6 0.6 CD2 C:HIS101 3.6 94.7 1.0 CE1 C:HIS101 3.6 99.2 1.0 N C:GLY34 3.9 85.7 1.0 N C:ASP35 4.0 74.7 1.0 C C:GLY34 4.2 76.6 1.0 SG C:CYS102 4.2 80.6 1.0 CA C:ASP35 4.2 69.9 1.0 CA C:CYS33 4.3 94.5 1.0 O C:GLY34 4.5 74.3 1.0 SG A:CYS321 4.5 73.2 1.0 C C:CYS33 4.6 90.7 1.0 CA C:GLY34 4.7 81.8 1.0 N C:CYS33 4.7 96.7 1.0 ND1 C:HIS101 4.7 0.1 1.0 CG C:HIS101 4.8 95.7 1.0

E.N.Marinoni, J.S.De Oliveira, Y.Nicolet, E.C.Raulfs, P.Amara, D.R.Dean, J.C.Fontecilla-Camps. (Iscs-Iscu)2 Complex Structures Provide Insights Into FE2S2 Biogenesis and Transfer. Angew.Chem.Int.Ed.Engl. V. 51 5439 2012.
ISSN: ISSN 1433-7851
PubMed: 22511353
DOI: 10.1002/ANIE.201201708