Iron in PDB 4h2l: Deer Mouse Hemoglobin in Hydrated Format

The structure of Deer Mouse Hemoglobin in Hydrated Format, PDB code: 4h2l was solved by N.Inoguchi, J.R.Oshlo, C.Natarajan, R.E.Weber, A.Fago, J.F.Storz, H.Moriyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.43 / 1.78
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	76.675, 80.061, 86.848, 90.00, 90.00, 90.00
R / Rfree (%)	16.2 / 20.1

The binding sites of Iron atom in the Deer Mouse Hemoglobin in Hydrated Format (pdb code 4h2l). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Deer Mouse Hemoglobin in Hydrated Format, PDB code: 4h2l:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Deer Mouse Hemoglobin in Hydrated Format


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Deer Mouse Hemoglobin in Hydrated Format within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:15.8 occ:1.00 FE A:HEM201 0.0 15.8 1.0 ND A:HEM201 2.0 14.1 1.0 NA A:HEM201 2.0 14.7 1.0 NC A:HEM201 2.1 13.7 1.0 NB A:HEM201 2.1 15.6 1.0 NE2 A:HIS87 2.2 15.9 1.0 O A:HOH410 2.3 14.3 1.0 C1D A:HEM201 3.0 16.8 1.0 C4C A:HEM201 3.0 14.4 1.0 C4D A:HEM201 3.1 15.6 1.0 C1A A:HEM201 3.1 15.9 1.0 C1B A:HEM201 3.1 17.1 1.0 C4A A:HEM201 3.1 17.6 1.0 C4B A:HEM201 3.1 14.6 1.0 C1C A:HEM201 3.1 14.4 1.0 CE1 A:HIS87 3.2 15.2 1.0 CD2 A:HIS87 3.2 15.5 1.0 CHD A:HEM201 3.4 16.6 1.0 CHA A:HEM201 3.4 17.3 1.0 CHB A:HEM201 3.5 14.7 1.0 CHC A:HEM201 3.5 14.4 1.0 NE2 A:HIS58 4.2 15.3 1.0 C2D A:HEM201 4.3 16.4 1.0 C3C A:HEM201 4.3 11.8 1.0 C3D A:HEM201 4.3 14.5 1.0 C2A A:HEM201 4.3 16.9 1.0 C3A A:HEM201 4.3 16.1 1.0 C2B A:HEM201 4.3 14.0 1.0 C2C A:HEM201 4.3 11.9 1.0 C3B A:HEM201 4.3 12.3 1.0 ND1 A:HIS87 4.3 15.6 1.0 CG A:HIS87 4.4 14.8 1.0 CE1 A:HIS58 4.5 19.4 1.0 CD1 A:LEU91 4.9 20.9 1.0 CG2 A:VAL62 5.0 11.6 1.0

Iron binding site 2 out of 2 in the Deer Mouse Hemoglobin in Hydrated Format


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Deer Mouse Hemoglobin in Hydrated Format within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:13.2 occ:1.00 FE B:HEM201 0.0 13.2 1.0 ND B:HEM201 2.0 11.8 1.0 NA B:HEM201 2.0 10.7 1.0 NB B:HEM201 2.0 12.4 1.0 NC B:HEM201 2.0 11.1 1.0 O B:HOH416 2.1 11.3 1.0 NE2 B:HIS92 2.3 12.5 1.0 C1D B:HEM201 3.0 12.6 1.0 C4C B:HEM201 3.1 13.3 1.0 C4A B:HEM201 3.1 10.8 1.0 C4D B:HEM201 3.1 12.7 1.0 C1A B:HEM201 3.1 14.9 1.0 C1B B:HEM201 3.1 13.8 1.0 C4B B:HEM201 3.1 11.4 1.0 C1C B:HEM201 3.1 12.2 1.0 CD2 B:HIS92 3.2 13.4 1.0 CE1 B:HIS92 3.3 14.7 1.0 CHD B:HEM201 3.4 12.5 1.0 CHB B:HEM201 3.4 11.2 1.0 CHA B:HEM201 3.5 14.4 1.0 CHC B:HEM201 3.5 10.4 1.0 NE2 B:HIS63 4.1 15.9 1.0 C3A B:HEM201 4.3 10.6 1.0 C2A B:HEM201 4.3 13.6 1.0 C3C B:HEM201 4.3 10.9 1.0 C2D B:HEM201 4.3 13.6 1.0 C3D B:HEM201 4.3 11.7 1.0 C2C B:HEM201 4.3 10.3 1.0 C2B B:HEM201 4.3 12.7 1.0 C3B B:HEM201 4.3 13.7 1.0 ND1 B:HIS92 4.4 14.7 1.0 CG B:HIS92 4.4 13.3 1.0 CE1 B:HIS63 4.6 16.2 1.0 CG2 B:VAL67 4.7 12.9 1.0

N.Inoguchi, J.R.Oshlo, C.Natarajan, R.E.Weber, A.Fago, J.F.Storz, H.Moriyama. Deer Mouse Hemoglobin Exhibits A Lowered Oxygen Affinity Owing to Mobility of the E Helix. Acta Crystallogr.,Sect.F V. 69 393 2013.
ISSN: ESSN 1744-3091
PubMed: 23545644
DOI: 10.1107/S1744309113005708