Iron in PDB 4iam: Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain

All present enzymatic activity of Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain:
4.6.1.2;

The structure of Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain, PDB code: 4iam was solved by V.Kumar, F.Van Den Akker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	86.70 / 1.99
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	122.626, 122.626, 122.626, 90.00, 90.00, 90.00
R / Rfree (%)	12.6 / 15.9

The binding sites of Iron atom in the Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain (pdb code 4iam). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain, PDB code: 4iam:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:25.1 occ:1.00 FE A:HEM501 0.0 25.1 1.0 NE2 A:HIS105 2.0 26.8 1.0 NC A:HEM501 2.0 25.2 1.0 ND A:HEM501 2.0 24.3 1.0 NB A:HEM501 2.1 24.6 1.0 NA A:HEM501 2.1 24.4 1.0 CE1 A:HIS105 2.9 26.6 1.0 C4D A:HEM501 3.0 22.6 1.0 C4B A:HEM501 3.0 21.6 1.0 C1C A:HEM501 3.1 21.7 1.0 C4C A:HEM501 3.1 22.6 1.0 C1D A:HEM501 3.1 22.8 1.0 C1A A:HEM501 3.1 21.8 1.0 CD2 A:HIS105 3.1 22.0 1.0 C1B A:HEM501 3.1 20.8 1.0 C4A A:HEM501 3.2 22.6 1.0 CHA A:HEM501 3.4 19.5 1.0 CHC A:HEM501 3.4 19.8 1.0 CHD A:HEM501 3.4 22.4 1.0 CHB A:HEM501 3.5 19.4 1.0 NE1 A:TRP74 3.7 23.2 1.0 ND1 A:HIS105 4.1 23.5 1.0 CG A:HIS105 4.2 21.3 1.0 C3D A:HEM501 4.3 24.3 1.0 C3C A:HEM501 4.3 27.0 1.0 C2C A:HEM501 4.3 23.9 1.0 C2D A:HEM501 4.3 26.2 1.0 C3B A:HEM501 4.3 25.8 1.0 C2A A:HEM501 4.3 19.6 1.0 C2B A:HEM501 4.3 24.1 1.0 C3A A:HEM501 4.3 20.2 1.0 CE2 A:TRP74 4.5 26.4 1.0 CD1 A:TRP74 4.6 19.9 1.0 CZ2 A:TRP74 4.7 25.2 1.0

Iron binding site 2 out of 2 in the Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of the C139A Mutant of Nostoc H-Nox Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe201 b:24.1 occ:1.00 FE B:HEM201 0.0 24.1 1.0 NE2 B:HIS105 2.0 23.4 1.0 NC B:HEM201 2.0 24.3 1.0 NA B:HEM201 2.1 25.2 1.0 NB B:HEM201 2.1 29.0 1.0 ND B:HEM201 2.1 21.6 1.0 CE1 B:HIS105 2.9 21.3 1.0 C1C B:HEM201 3.0 26.9 1.0 C4B B:HEM201 3.1 25.0 1.0 C4C B:HEM201 3.1 24.7 1.0 C1A B:HEM201 3.1 26.5 1.0 C4D B:HEM201 3.1 23.1 1.0 C4A B:HEM201 3.1 26.6 1.0 CD2 B:HIS105 3.1 17.6 1.0 C1B B:HEM201 3.1 27.2 1.0 C1D B:HEM201 3.1 26.6 1.0 CHC B:HEM201 3.4 27.8 1.0 CHA B:HEM201 3.5 19.0 1.0 CHB B:HEM201 3.5 27.4 1.0 CHD B:HEM201 3.5 21.5 1.0 NE1 B:TRP74 3.8 23.6 1.0 ND1 B:HIS105 4.1 24.6 1.0 CG B:HIS105 4.2 23.8 1.0 C2C B:HEM201 4.2 29.0 1.0 C3C B:HEM201 4.2 25.9 1.0 C3A B:HEM201 4.3 27.4 1.0 C2A B:HEM201 4.3 25.2 1.0 C3D B:HEM201 4.3 23.8 1.0 C3B B:HEM201 4.3 27.6 1.0 C2D B:HEM201 4.3 24.9 1.0 C2B B:HEM201 4.3 25.3 1.0 CE2 B:TRP74 4.6 24.4 1.0 CZ2 B:TRP74 4.7 28.2 1.0 CD1 B:TRP74 4.8 28.8 1.0 CD B:PRO118 4.9 24.5 1.0

V.Kumar, F.Martin, M.G.Hahn, M.Schaefer, J.S.Stamler, J.P.Stasch, F.Van Den Akker. Insights Into Bay 60-2770 Activation and S-Nitrosylation-Dependent Desensitization of Soluble Guanylyl Cyclase Via Crystal Structures of Homologous Nostoc H-Nox Domain Complexes. Biochemistry V. 52 3601 2013.
ISSN: ISSN 0006-2960
PubMed: 23614626
DOI: 10.1021/BI301657W