Iron in PDB 4l2a: X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II)

All present enzymatic activity of X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II):
1.15.1.1;

The structure of X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II), PDB code: 4l2a was solved by A.Merlino, I.Russo Krauss, F.Sica, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.00 / 2.06
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.622, 103.736, 50.244, 90.00, 108.29, 90.00
R / Rfree (%)	19 / 24

The binding sites of Iron atom in the X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II) (pdb code 4l2a). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II), PDB code: 4l2a:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II) within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe5000 b:17.0 occ:1.00 OD2 A:ASP157 2.0 14.1 1.0 NE2 A:HIS73 2.0 17.4 1.0 NE2 A:HIS161 2.1 18.3 1.0 NE2 A:HIS26 2.1 13.1 1.0 O A:HOH5101 2.2 17.7 1.0 CD2 A:HIS73 2.9 14.7 1.0 CD2 A:HIS161 3.0 18.6 1.0 CE1 A:HIS26 3.0 13.2 1.0 CG A:ASP157 3.1 13.8 1.0 CE1 A:HIS73 3.1 16.6 1.0 CE1 A:HIS161 3.1 17.1 1.0 CD2 A:HIS26 3.2 14.2 1.0 OD1 A:ASP157 3.5 12.8 1.0 CG A:HIS73 4.1 14.5 1.0 ND1 A:HIS73 4.1 14.2 1.0 ND1 A:HIS26 4.2 14.1 1.0 CG A:HIS161 4.2 18.3 1.0 ND1 A:HIS161 4.2 19.9 1.0 CG A:HIS26 4.3 15.8 1.0 CB A:ASP157 4.4 15.4 1.0 CH2 A:TRP122 4.4 13.6 1.0 CB A:TRP159 4.4 14.0 1.0 CZ2 A:TRP122 4.6 13.2 1.0 CG A:TRP159 4.7 15.4 1.0 CB A:ALA162 4.9 11.9 1.0

Iron binding site 2 out of 2 in the X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of X-Ray Structure of the C57R Mutant of the Iron Superoxide Dismutase From Pseudoalteromonas Haloplanktis (Crystal Form II) within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe5001 b:16.8 occ:1.00 OD2 B:ASP157 2.0 19.8 1.0 NE2 B:HIS73 2.0 16.0 1.0 NE2 B:HIS161 2.2 15.4 1.0 NE2 B:HIS26 2.2 14.0 1.0 O B:HOH5102 2.3 18.6 1.0 CD2 B:HIS73 2.9 14.5 1.0 CG B:ASP157 3.0 18.5 1.0 CE1 B:HIS73 3.1 15.1 1.0 CE1 B:HIS26 3.1 16.1 1.0 CD2 B:HIS161 3.1 14.0 1.0 CD2 B:HIS26 3.2 13.3 1.0 CE1 B:HIS161 3.2 14.5 1.0 OD1 B:ASP157 3.4 16.7 1.0 CG B:HIS73 4.1 12.1 1.0 ND1 B:HIS73 4.1 15.1 1.0 ND1 B:HIS26 4.2 14.0 1.0 CG B:HIS26 4.2 15.3 1.0 CH2 B:TRP122 4.3 15.2 1.0 CG B:HIS161 4.3 16.0 1.0 ND1 B:HIS161 4.3 17.3 1.0 CB B:ASP157 4.3 19.3 1.0 CB B:TRP159 4.4 14.5 1.0 CZ2 B:TRP122 4.5 12.4 1.0 CG B:TRP159 4.7 13.0 1.0 CB B:ALA162 4.8 14.2 1.0

A.Merlino, I.Russo Krauss, I.Castellano, M.R.Ruocco, A.Capasso, E.De Vendittis, B.Rossi, F.Sica. Structural and Denaturation Studies of Two Mutants of A Cold Adapted Superoxide Dismutase Point to the Importance of Electrostatic Interactions in Protein Stability. Biochim.Biophys.Acta V.1844 632 2014.
ISSN: ISSN 0006-3002
PubMed: 24440460
DOI: 10.1016/J.BBAPAP.2014.01.007