Iron in PDB 4lyx: Five Minutes Iron Loaded Frog M Ferritin

All present enzymatic activity of Five Minutes Iron Loaded Frog M Ferritin:
1.16.3.1;

The structure of Five Minutes Iron Loaded Frog M Ferritin, PDB code: 4lyx was solved by S.Mangani, F.Di Pisa, C.Pozzi, P.Turano, D.Lalli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.22 / 1.23
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	184.180, 184.180, 184.180, 90.00, 90.00, 90.00
R / Rfree (%)	16.1 / 17.5

The structure of Five Minutes Iron Loaded Frog M Ferritin also contains other interesting chemical elements:

Magnesium	(Mg)	11 atoms
Chlorine	(Cl)	12 atoms

The binding sites of Iron atom in the Five Minutes Iron Loaded Frog M Ferritin (pdb code 4lyx). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Five Minutes Iron Loaded Frog M Ferritin, PDB code: 4lyx:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Five Minutes Iron Loaded Frog M Ferritin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Five Minutes Iron Loaded Frog M Ferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:12.2 occ:0.50 OE1 A:GLU58 1.9 13.3 0.5 OE1 A:GLU23 2.0 13.2 0.7 OE2 A:GLU23 2.1 8.7 0.3 OE1 A:GLU58 2.1 13.7 0.5 ND1 A:HIS61 2.1 12.4 0.5 O A:HOH481 2.1 23.3 1.0 OE1 A:GLU23 2.2 8.8 0.3 O A:HOH301 2.3 12.6 0.6 CD A:GLU23 2.4 8.2 0.3 CD A:GLU58 2.8 13.1 0.5 CD A:GLU23 3.0 12.3 0.7 CE1 A:HIS61 3.0 12.6 0.5 CD A:GLU58 3.1 12.8 0.5 CG A:HIS61 3.2 11.9 0.5 MG A:MG211 3.2 9.1 0.3 OE2 A:GLU23 3.3 13.5 0.7 OE2 A:GLU58 3.3 13.6 0.5 OE2 A:GLU58 3.5 13.7 0.5 CD2 A:HIS61 3.5 13.7 0.5 O A:HOH664 3.5 11.9 0.3 CB A:HIS61 3.6 11.3 0.5 FE A:FE2202 3.6 16.0 0.3 CG A:GLU23 3.9 8.4 0.3 CB A:HIS61 3.9 11.2 0.5 CG A:HIS61 4.0 12.4 0.5 CG1 A:VAL106 4.0 10.7 1.0 CG A:GLU58 4.1 12.1 0.5 NE2 A:HIS61 4.2 12.3 0.5 CD2 A:HIS61 4.3 12.2 0.5 CG A:GLU23 4.3 11.8 0.7 OE1 A:GLN137 4.3 19.9 1.0 CB A:GLU58 4.4 11.7 0.5 CA A:GLU58 4.4 11.2 0.5 CG A:GLU58 4.4 12.3 0.5 CA A:GLU58 4.5 11.0 0.5 OE2 A:GLU136 4.6 16.5 0.5 NE2 A:HIS61 4.6 14.2 0.5 CB A:GLU23 4.7 10.6 0.7 CB A:GLU58 4.7 11.4 0.5 CB A:GLU23 4.7 8.2 0.3 O A:HOH497 4.9 30.4 1.0 CA A:GLU23 5.0 10.1 0.7 CA A:GLU23 5.0 8.2 0.3

Iron binding site 2 out of 3 in the Five Minutes Iron Loaded Frog M Ferritin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Five Minutes Iron Loaded Frog M Ferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe202 b:16.0 occ:0.30 OE2 A:GLU58 1.8 13.7 0.5 O A:HOH301 1.9 12.6 0.6 OE1 A:GLU103 2.2 11.2 0.5 OE2 A:GLU103 2.3 13.3 0.5 OE2 A:GLU103 2.4 11.4 0.5 CD A:GLU103 2.6 11.6 0.5 O A:HOH441 2.6 27.8 1.0 OE1 A:GLU103 2.6 13.6 0.5 OE2 A:GLU58 2.6 13.6 0.5 OE1 A:GLU58 2.8 13.3 0.5 CD A:GLU58 2.8 13.1 0.5 CD A:GLU103 2.8 12.6 0.5 O A:HOH664 2.8 11.9 0.3 O A:HOH497 2.9 30.4 1.0 CD A:GLU58 2.9 12.8 0.5 OE1 A:GLU58 3.4 13.7 0.5 MG A:MG211 3.5 9.1 0.3 FE A:FE2201 3.6 12.2 0.5 CG A:GLU58 3.9 12.1 0.5 NE2 A:GLN137 4.0 15.7 1.0 CG A:GLU103 4.1 10.7 0.5 CG A:GLU58 4.1 12.3 0.5 OE1 A:GLN137 4.2 19.9 1.0 CG A:GLU103 4.3 12.2 0.5 CE2 A:TYR30 4.3 8.4 0.5 CE1 A:HIS61 4.3 12.6 0.5 CE2 A:TYR30 4.4 11.0 0.5 CD A:GLN137 4.6 15.9 1.0 ND1 A:HIS61 4.6 12.4 0.5 OD2 A:ASP140 4.6 21.6 1.0 OH A:TYR30 4.7 8.9 0.5 O A:HOH481 4.7 23.3 1.0 OH A:TYR30 4.7 12.4 0.5 CB A:GLU103 4.8 10.4 0.5 ND1 A:HIS54 4.8 16.0 0.4 CZ A:TYR30 5.0 8.3 0.5

Iron binding site 3 out of 3 in the Five Minutes Iron Loaded Frog M Ferritin


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Five Minutes Iron Loaded Frog M Ferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe203 b:13.4 occ:0.10 CL A:CL220 1.6 11.4 0.2 CE1 A:HIS169 2.7 15.1 0.5 NE2 A:HIS169 2.8 11.2 0.5 CE1 A:HIS169 3.1 12.8 0.5 NE2 A:HIS169 3.4 18.4 0.5 CL A:CL219 3.5 17.6 0.2 ND1 A:HIS169 3.8 17.6 0.5 CD2 A:HIS169 4.1 8.6 0.5 ND1 A:HIS169 4.4 11.5 0.5 CD2 A:HIS169 4.6 17.6 0.5 CG A:HIS169 4.8 15.4 0.5 CG A:HIS169 4.9 8.9 0.5

S.Mangani, F.Di Pisa, C.Pozzi, P.Turano, D.Lalli. Crystallographic Snapshots of Iron in Frog M Ferritin To Be Published.