Iron in PDB 4n4o: Nitrosomonas Europea Hao Soaked in NH2OH

All present enzymatic activity of Nitrosomonas Europea Hao Soaked in NH2OH:
1.7.2.6;

The structure of Nitrosomonas Europea Hao Soaked in NH2OH, PDB code: 4n4o was solved by W.J.Maalcke, A.Dietl, S.J.Marritt, J.N.Butt, M.S.M.Jetten, J.T.Keltjens, T.R.M.B.Barends, B.Kartal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.95 / 2.47
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	140.090, 141.960, 106.480, 90.00, 90.00, 90.00
R / Rfree (%)	22.4 / 27.3

The structure of Nitrosomonas Europea Hao Soaked in NH2OH also contains other interesting chemical elements:

Potassium

(K)

1 atom

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 24;

Binding sites:

The binding sites of Iron atom in the Nitrosomonas Europea Hao Soaked in NH2OH (pdb code 4n4o). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 24 binding sites of Iron where determined in the Nitrosomonas Europea Hao Soaked in NH2OH, PDB code: 4n4o:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Iron binding site 1 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe601 b:27.9 occ:1.00 FE A:HEM601 0.0 27.9 1.0 NE2 A:HIS267 1.9 27.3 1.0 NE2 A:HIS347 1.9 29.5 1.0 NA A:HEM601 2.1 28.8 1.0 NC A:HEM601 2.1 28.4 1.0 NB A:HEM601 2.1 28.1 1.0 ND A:HEM601 2.1 28.3 1.0 CE1 A:HIS347 2.7 30.1 1.0 CE1 A:HIS267 2.8 27.4 1.0 CD2 A:HIS267 2.9 27.3 1.0 CD2 A:HIS347 3.0 30.4 1.0 C4A A:HEM601 3.1 28.6 1.0 C4C A:HEM601 3.1 28.0 1.0 C1B A:HEM601 3.1 28.1 1.0 C1A A:HEM601 3.1 29.1 1.0 C1D A:HEM601 3.1 28.4 1.0 C1C A:HEM601 3.1 28.0 1.0 C4D A:HEM601 3.1 28.7 1.0 C4B A:HEM601 3.1 27.9 1.0 CHB A:HEM601 3.4 28.4 1.0 CHD A:HEM601 3.4 28.1 1.0 CHA A:HEM601 3.5 28.8 1.0 CHC A:HEM601 3.5 27.8 1.0 ND1 A:HIS347 3.9 30.1 1.0 ND1 A:HIS267 3.9 27.4 1.0 CG A:HIS267 4.0 27.1 1.0 CG A:HIS347 4.1 30.9 1.0 C3A A:HEM601 4.3 29.1 1.0 C2B A:HEM601 4.3 27.8 1.0 C2A A:HEM601 4.3 29.3 1.0 C3C A:HEM601 4.3 27.8 1.0 C3B A:HEM601 4.3 27.8 1.0 C2C A:HEM601 4.3 28.0 1.0 C2D A:HEM601 4.3 28.6 1.0 C3D A:HEM601 4.3 28.8 1.0 O A:HOH755 4.7 39.9 1.0

Iron binding site 2 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe602 b:35.6 occ:1.00 FE A:HEM602 0.0 35.6 1.0 NE2 A:HIS200 1.9 35.5 1.0 NE2 A:HIS123 1.9 37.7 1.0 NC A:HEM602 2.0 36.3 1.0 NB A:HEM602 2.0 35.9 1.0 NA A:HEM602 2.1 36.1 1.0 ND A:HEM602 2.1 36.5 1.0 CE1 A:HIS123 2.8 38.7 1.0 CE1 A:HIS200 2.8 35.7 1.0 CD2 A:HIS200 2.9 35.9 1.0 CD2 A:HIS123 3.0 38.9 1.0 C4B A:HEM602 3.1 35.7 1.0 C4C A:HEM602 3.1 36.4 1.0 C1C A:HEM602 3.1 36.1 1.0 C1B A:HEM602 3.1 35.6 1.0 C1A A:HEM602 3.1 36.2 1.0 C4A A:HEM602 3.1 35.9 1.0 C1D A:HEM602 3.1 36.7 1.0 C4D A:HEM602 3.1 36.6 1.0 CHA A:HEM602 3.4 36.6 1.0 CHC A:HEM602 3.4 35.9 1.0 CHD A:HEM602 3.4 36.6 1.0 CHB A:HEM602 3.4 35.6 1.0 ND1 A:HIS123 3.9 38.9 1.0 ND1 A:HIS200 3.9 35.6 1.0 CG A:HIS200 4.0 36.2 1.0 CG A:HIS123 4.0 39.4 1.0 C3B A:HEM602 4.3 35.4 1.0 C3C A:HEM602 4.3 36.2 1.0 C2B A:HEM602 4.3 35.5 1.0 C2C A:HEM602 4.3 36.0 1.0 C2A A:HEM602 4.3 36.0 1.0 C3A A:HEM602 4.3 36.0 1.0 C2D A:HEM602 4.3 37.0 1.0 C3D A:HEM602 4.3 37.0 1.0

Iron binding site 3 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe603 b:30.9 occ:1.00 FE A:HEM603 0.0 30.9 1.0 NE2 A:HIS107 1.9 32.9 1.0 NE2 A:HIS184 1.9 35.2 1.0 NA A:HEM603 2.0 30.0 1.0 NC A:HEM603 2.1 30.8 1.0 NB A:HEM603 2.1 30.5 1.0 ND A:HEM603 2.1 30.1 1.0 CE1 A:HIS184 2.8 36.1 1.0 CE1 A:HIS107 2.8 33.3 1.0 CD2 A:HIS107 2.9 33.4 1.0 CD2 A:HIS184 2.9 36.4 1.0 C4A A:HEM603 3.0 29.9 1.0 C1A A:HEM603 3.1 29.6 1.0 C4C A:HEM603 3.1 31.1 1.0 C1C A:HEM603 3.1 30.9 1.0 C1B A:HEM603 3.1 30.6 1.0 C1D A:HEM603 3.1 30.4 1.0 C4B A:HEM603 3.1 30.7 1.0 C4D A:HEM603 3.1 30.0 1.0 CHB A:HEM603 3.4 30.3 1.0 CHD A:HEM603 3.4 31.0 1.0 CHA A:HEM603 3.4 29.5 1.0 CHC A:HEM603 3.4 30.6 1.0 ND1 A:HIS184 3.9 36.5 1.0 ND1 A:HIS107 3.9 33.2 1.0 CG A:HIS107 4.0 33.6 1.0 CG A:HIS184 4.0 37.1 1.0 C3A A:HEM603 4.3 29.4 1.0 C2A A:HEM603 4.3 29.2 1.0 C3C A:HEM603 4.3 31.4 1.0 C2C A:HEM603 4.3 31.5 1.0 C2B A:HEM603 4.3 30.6 1.0 C3B A:HEM603 4.3 30.8 1.0 C2D A:HEM603 4.3 30.1 1.0 C3D A:HEM603 4.3 30.1 1.0

Iron binding site 4 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe604 b:28.0 occ:1.00 FE A:HEM604 0.0 28.0 1.0 NE2 A:HIS270 1.9 29.3 1.0 NE2 A:HIS173 1.9 33.0 1.0 NB A:HEM604 2.0 28.2 1.0 NA A:HEM604 2.1 27.5 1.0 NC A:HEM604 2.1 28.2 1.0 ND A:HEM604 2.1 27.4 1.0 CE1 A:HIS173 2.6 34.1 1.0 CD2 A:HIS270 2.8 29.5 1.0 CE1 A:HIS270 2.9 29.2 1.0 C1B A:HEM604 3.1 28.4 1.0 CD2 A:HIS173 3.1 34.3 1.0 C4B A:HEM604 3.1 28.6 1.0 C4A A:HEM604 3.1 27.3 1.0 C1C A:HEM604 3.1 28.6 1.0 C4D A:HEM604 3.1 27.0 1.0 C4C A:HEM604 3.1 28.4 1.0 C1A A:HEM604 3.1 26.9 1.0 C1D A:HEM604 3.1 27.3 1.0 CHB A:HEM604 3.4 27.9 1.0 CHA A:HEM604 3.4 26.6 1.0 CHC A:HEM604 3.4 28.6 1.0 CHD A:HEM604 3.4 27.7 1.0 ND1 A:HIS173 3.8 34.6 1.0 CG A:HIS270 4.0 29.3 1.0 ND1 A:HIS270 4.0 29.3 1.0 CG A:HIS173 4.1 35.1 1.0 C2B A:HEM604 4.3 28.7 1.0 C3B A:HEM604 4.3 29.1 1.0 C2C A:HEM604 4.3 28.9 1.0 C3C A:HEM604 4.3 29.0 1.0 C3A A:HEM604 4.3 26.8 1.0 C2A A:HEM604 4.3 26.8 1.0 C3D A:HEM604 4.3 27.0 1.0 C2D A:HEM604 4.3 26.8 1.0

Iron binding site 5 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe605 b:23.1 occ:1.00 FE A:HEM605 0.0 23.1 1.0 NE2 A:HIS338 1.9 26.1 1.0 NE2 A:HIS483 1.9 21.8 1.0 NB A:HEM605 2.0 23.0 1.0 NA A:HEM605 2.0 22.9 1.0 NC A:HEM605 2.1 23.4 1.0 ND A:HEM605 2.1 22.6 1.0 CE1 A:HIS338 2.6 26.9 1.0 CD2 A:HIS483 2.8 20.9 1.0 CE1 A:HIS483 2.9 21.0 1.0 C4A A:HEM605 3.0 22.9 1.0 C1B A:HEM605 3.0 23.0 1.0 C1A A:HEM605 3.0 22.8 1.0 C4B A:HEM605 3.0 23.3 1.0 C4C A:HEM605 3.1 23.5 1.0 C1C A:HEM605 3.1 23.6 1.0 CD2 A:HIS338 3.1 26.8 1.0 C1D A:HEM605 3.1 22.7 1.0 C4D A:HEM605 3.1 22.8 1.0 CHB A:HEM605 3.4 22.8 1.0 CHA A:HEM605 3.4 22.8 1.0 CHD A:HEM605 3.4 23.2 1.0 CHC A:HEM605 3.4 23.2 1.0 ND1 A:HIS338 3.8 27.1 1.0 CG A:HIS483 3.9 20.8 1.0 ND1 A:HIS483 4.0 20.6 1.0 CG A:HIS338 4.1 27.7 1.0 C3A A:HEM605 4.2 22.7 1.0 C2A A:HEM605 4.2 22.9 1.0 C2B A:HEM605 4.3 23.1 1.0 C3B A:HEM605 4.3 23.4 1.0 C2C A:HEM605 4.3 23.9 1.0 C3C A:HEM605 4.3 24.1 1.0 C2D A:HEM605 4.3 22.6 1.0 C3D A:HEM605 4.3 22.3 1.0 ND2 A:ASN294 4.7 20.9 1.0

Iron binding site 6 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe606 b:23.9 occ:1.00 FE A:HEM606 0.0 23.9 1.0 NE2 A:HIS287 1.9 22.6 1.0 NE2 A:HIS228 1.9 27.5 1.0 NC A:HEM606 2.0 23.0 1.0 NB A:HEM606 2.0 23.1 1.0 NA A:HEM606 2.1 23.3 1.0 ND A:HEM606 2.1 23.4 1.0 CE1 A:HIS287 2.7 21.8 1.0 CE1 A:HIS228 2.8 28.4 1.0 CD2 A:HIS228 2.9 28.3 1.0 CD2 A:HIS287 3.0 21.9 1.0 C4C A:HEM606 3.1 23.0 1.0 C4B A:HEM606 3.1 23.2 1.0 C1C A:HEM606 3.1 23.1 1.0 C1D A:HEM606 3.1 22.8 1.0 C1B A:HEM606 3.1 23.1 1.0 C4D A:HEM606 3.1 23.5 1.0 C1A A:HEM606 3.1 23.6 1.0 C4A A:HEM606 3.1 23.4 1.0 CHD A:HEM606 3.4 22.7 1.0 CHC A:HEM606 3.4 23.2 1.0 CHA A:HEM606 3.4 23.6 1.0 CHB A:HEM606 3.4 23.3 1.0 ND1 A:HIS287 3.9 21.6 1.0 ND1 A:HIS228 3.9 28.6 1.0 CG A:HIS228 4.0 28.8 1.0 CG A:HIS287 4.0 21.8 1.0 C3C A:HEM606 4.3 22.8 1.0 C3B A:HEM606 4.3 23.4 1.0 C2C A:HEM606 4.3 22.7 1.0 C2B A:HEM606 4.3 23.4 1.0 C2D A:HEM606 4.3 22.9 1.0 C3D A:HEM606 4.3 23.3 1.0 C2A A:HEM606 4.3 23.4 1.0 C3A A:HEM606 4.3 23.1 1.0 ND2 A:ASN259 4.6 25.2 1.0

Iron binding site 7 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe607 b:26.9 occ:1.00 FE A:HEM607 0.0 26.9 1.0 NE2 A:HIS388 1.9 28.8 1.0 NE2 A:HIS303 1.9 26.1 1.0 NC A:HEM607 2.0 27.0 1.0 NA A:HEM607 2.0 26.9 1.0 NB A:HEM607 2.1 26.9 1.0 ND A:HEM607 2.1 26.9 1.0 CE1 A:HIS303 2.6 26.0 1.0 CE1 A:HIS388 2.8 29.3 1.0 CD2 A:HIS388 2.9 29.5 1.0 CD2 A:HIS303 3.0 25.4 1.0 C1C A:HEM607 3.1 27.0 1.0 C1A A:HEM607 3.1 27.0 1.0 C4C A:HEM607 3.1 27.1 1.0 C4A A:HEM607 3.1 26.9 1.0 C4B A:HEM607 3.1 27.0 1.0 C4D A:HEM607 3.1 26.9 1.0 C1B A:HEM607 3.1 27.0 1.0 C1D A:HEM607 3.1 26.8 1.0 CHA A:HEM607 3.4 26.8 1.0 CHC A:HEM607 3.4 27.0 1.0 CHD A:HEM607 3.4 26.9 1.0 CHB A:HEM607 3.4 26.8 1.0 ND1 A:HIS303 3.8 25.6 1.0 ND1 A:HIS388 3.9 29.4 1.0 CG A:HIS388 4.0 29.7 1.0 CG A:HIS303 4.0 25.2 1.0 C2C A:HEM607 4.3 27.2 1.0 C3C A:HEM607 4.3 27.4 1.0 C2A A:HEM607 4.3 27.1 1.0 C3A A:HEM607 4.3 26.9 1.0 C3B A:HEM607 4.3 27.2 1.0 C2B A:HEM607 4.3 27.1 1.0 C3D A:HEM607 4.3 26.9 1.0 C2D A:HEM607 4.3 26.9 1.0 O A:HOH950 4.9 17.6 1.0 CG A:PRO332 4.9 28.5 1.0

Iron binding site 8 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 8 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe608 b:24.6 occ:1.00 FE A:HEC608 0.0 24.6 1.0 NB A:HEC608 1.9 24.6 1.0 NA A:HEC608 1.9 24.9 1.0 ND A:HEC608 1.9 24.7 1.0 NC A:HEC608 1.9 24.6 1.0 N E:HOA610 1.9 24.1 1.0 NE2 E:HIS257 2.1 24.2 1.0 CE1 E:HIS257 2.9 24.3 1.0 C1B A:HEC608 2.9 24.7 1.0 C4A A:HEC608 2.9 25.2 1.0 C1D A:HEC608 2.9 24.6 1.0 C4D A:HEC608 3.0 24.5 1.0 C1C A:HEC608 3.0 24.6 1.0 C1A A:HEC608 3.0 25.3 1.0 C4C A:HEC608 3.0 24.6 1.0 C4B A:HEC608 3.0 24.2 1.0 O E:HOA610 3.1 23.9 1.0 CHB A:HEC608 3.2 25.0 1.0 CD2 E:HIS257 3.3 24.4 1.0 CHD A:HEC608 3.4 24.5 1.0 CHA A:HEC608 3.4 24.9 1.0 CHC A:HEC608 3.5 24.1 1.0 OH A:TYR491 3.8 23.9 1.0 ND1 E:HIS257 4.1 24.2 1.0 C2B A:HEC608 4.1 24.4 1.0 C2D A:HEC608 4.2 24.3 1.0 C3D A:HEC608 4.2 24.4 1.0 C3A A:HEC608 4.2 25.4 1.0 C3C A:HEC608 4.2 25.0 1.0 C3B A:HEC608 4.2 24.3 1.0 C2C A:HEC608 4.2 24.7 1.0 C2A A:HEC608 4.2 25.6 1.0 CG E:HIS257 4.3 24.6 1.0 CZ A:TYR491 4.4 23.1 1.0 CE2 A:TYR491 4.6 22.9 1.0 CMD E:HEM607 4.7 19.8 1.0 NE2 E:HIS292 4.8 18.6 1.0

Iron binding site 9 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 9 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe601 b:22.1 occ:1.00 FE C:HEC601 0.0 22.1 1.0 NB C:HEC601 1.9 22.0 1.0 NA C:HEC601 1.9 22.1 1.0 ND C:HEC601 1.9 21.8 1.0 NC C:HEC601 1.9 21.7 1.0 N A:HOA610 1.9 21.9 1.0 NE2 A:HIS257 2.1 23.3 1.0 C1B C:HEC601 2.9 22.1 1.0 CE1 A:HIS257 2.9 23.2 1.0 C4A C:HEC601 2.9 22.2 1.0 C4D C:HEC601 2.9 21.6 1.0 O A:HOA610 3.0 22.1 1.0 C1C C:HEC601 3.0 21.6 1.0 C1D C:HEC601 3.0 21.5 1.0 C1A C:HEC601 3.0 22.2 1.0 C4C C:HEC601 3.0 21.5 1.0 C4B C:HEC601 3.0 21.5 1.0 CD2 A:HIS257 3.3 23.7 1.0 CHB C:HEC601 3.3 22.1 1.0 CHD C:HEC601 3.3 21.6 1.0 CHA C:HEC601 3.4 21.8 1.0 CHC C:HEC601 3.5 21.5 1.0 OH C:TYR491 3.6 20.7 1.0 ND1 A:HIS257 4.1 23.4 1.0 C2B C:HEC601 4.1 21.6 1.0 C3D C:HEC601 4.2 21.7 1.0 C3A C:HEC601 4.2 22.5 1.0 C2D C:HEC601 4.2 21.6 1.0 C2C C:HEC601 4.2 21.6 1.0 C3B C:HEC601 4.2 21.6 1.0 C2A C:HEC601 4.2 22.4 1.0 C3C C:HEC601 4.2 21.5 1.0 CG A:HIS257 4.3 24.0 1.0 CZ C:TYR491 4.3 20.4 1.0 CE2 C:TYR491 4.4 20.7 1.0 CMD A:HEM606 4.8 22.4 1.0 NE2 A:HIS292 4.9 20.7 1.0

Iron binding site 10 out of 24 in the Nitrosomonas Europea Hao Soaked in NH2OH


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 10 of Nitrosomonas Europea Hao Soaked in NH2OH within 5.0Å range: probe atom residue distance (Å) B Occ C:Fe602 b:26.8 occ:1.00 FE C:HEM602 0.0 26.8 1.0 NE2 C:HIS267 1.9 27.7 1.0 NE2 C:HIS347 1.9 29.2 1.0 NC C:HEM602 2.1 26.8 1.0 NA C:HEM602 2.1 27.2 1.0 NB C:HEM602 2.1 26.7 1.0 ND C:HEM602 2.1 26.7 1.0 CE1 C:HIS347 2.7 29.5 1.0 CD2 C:HIS267 2.9 27.9 1.0 CE1 C:HIS267 2.9 27.7 1.0 CD2 C:HIS347 3.0 29.7 1.0 C4C C:HEM602 3.1 26.6 1.0 C1D C:HEM602 3.1 26.6 1.0 C1B C:HEM602 3.1 26.9 1.0 C4A C:HEM602 3.1 27.1 1.0 C4D C:HEM602 3.1 26.9 1.0 C1C C:HEM602 3.1 26.7 1.0 C4B C:HEM602 3.1 27.0 1.0 C1A C:HEM602 3.1 27.3 1.0 CHD C:HEM602 3.4 26.6 1.0 CHB C:HEM602 3.4 27.0 1.0 CHA C:HEM602 3.5 27.3 1.0 CHC C:HEM602 3.5 26.8 1.0 ND1 C:HIS347 3.9 29.4 1.0 ND1 C:HIS267 4.0 28.0 1.0 CG C:HIS267 4.0 28.2 1.0 CG C:HIS347 4.1 30.1 1.0 C3C C:HEM602 4.3 26.8 1.0 C2D C:HEM602 4.3 26.5 1.0 C2B C:HEM602 4.3 26.9 1.0 C3D C:HEM602 4.3 26.4 1.0 C3B C:HEM602 4.3 26.9 1.0 C3A C:HEM602 4.3 27.4 1.0 C2C C:HEM602 4.3 26.8 1.0 C2A C:HEM602 4.3 27.5 1.0 O C:HOH708 4.8 28.6 1.0

W.J.Maalcke, A.Dietl, S.J.Marritt, J.N.Butt, M.S.Jetten, J.T.Keltjens, T.R.Barends, B.Kartal. Structural Basis of Biological No Generation By Octaheme Oxidoreductases. J.Biol.Chem. V. 289 1228 2014.
ISSN: ISSN 0021-9258
PubMed: 24302732
DOI: 10.1074/JBC.M113.525147