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Iron in PDB 4v0j: The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)

Enzymatic activity of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)

All present enzymatic activity of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis):
1.14.19.2;

Protein crystallography data

The structure of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis), PDB code: 4v0j was solved by M.Moche, J.Guy, E.Whittle, Y.Lindqvist, J.Shanklin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 119.52 / 2.80
Space group P 31
Cell size a, b, c (Å), α, β, γ (°) 139.719, 139.719, 86.871, 90.00, 90.00, 120.00
R / Rfree (%) 21.183 / 26.418

Iron Binding Sites:

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Iron atom in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) (pdb code 4v0j). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 12 binding sites of Iron where determined in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis), PDB code: 4v0j:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Iron binding site 1 out of 12 in 4v0j

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Iron binding site 1 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe364

b:78.9
occ:1.00
 OE2 A:GLU105 1.8 80.7 1.0
OE1 A:GLU143 2.0 62.9 1.0
OE2 A:GLU229 2.2 73.6 1.0
ND1 A:HIS146 2.2 50.8 1.0
OE1 A:GLU105 2.7 66.9 1.0
CD A:GLU105 2.7 62.0 1.0
CD A:GLU229 2.8 67.7 1.0
CE1 A:HIS146 2.9 51.1 1.0
OE1 A:GLU229 3.0 59.7 1.0
CD A:GLU143 3.1 48.2 1.0
CG A:HIS146 3.4 50.8 1.0
OE2 A:GLU143 3.6 49.1 1.0
FE A:FE2365 3.7 69.9 1.0
CB A:HIS146 4.0 46.9 1.0
NE2 A:HIS146 4.1 47.8 1.0
CG A:GLU229 4.2 68.5 1.0
CG A:GLU105 4.3 53.6 1.0
CG A:GLU143 4.4 47.6 1.0
CD2 A:HIS146 4.4 46.4 1.0
NE2 A:HIS203 4.4 79.0 1.0
CG2 A:ILE225 4.4 60.3 1.0
CB A:GLU143 4.6 44.9 1.0
CA A:GLU143 4.7 43.4 1.0
CD2 A:HIS203 4.8 76.3 1.0
O A:GLU143 4.9 51.4 1.0

Iron binding site 2 out of 12 in 4v0j

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Iron binding site 2 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe365

b:69.9
occ:1.00
 OE1 A:GLU229 1.9 59.7 1.0
OE2 A:GLU143 1.9 49.1 1.0
OE1 A:GLU196 2.0 54.7 1.0
OE2 A:GLU196 2.6 57.1 1.0
CD A:GLU196 2.7 55.4 1.0
ND1 A:HIS232 2.7 61.2 1.0
CD A:GLU229 2.8 67.7 1.0
CD A:GLU143 2.9 48.2 1.0
OE1 A:GLU143 3.3 62.9 1.0
OE2 A:GLU229 3.4 73.6 1.0
CE1 A:HIS232 3.6 57.8 1.0
FE A:FE2364 3.7 78.9 1.0
CG A:HIS232 3.7 57.4 1.0
CA A:GLU229 3.9 52.5 1.0
NE1 A:TRP139 4.0 52.8 1.0
CB A:HIS232 4.0 52.3 1.0
CG A:GLU229 4.0 68.5 1.0
CB A:GLU229 4.1 60.5 1.0
CG A:GLU196 4.2 59.5 1.0
CG A:GLU143 4.2 47.6 1.0
OG1 A:THR199 4.3 60.8 1.0
CZ2 A:TRP139 4.5 56.6 1.0
CE2 A:TRP139 4.5 54.7 1.0
CE1 A:HIS146 4.7 51.1 1.0
N A:GLU229 4.7 54.3 1.0
NE2 A:HIS232 4.8 57.4 1.0
CB A:GLU196 4.8 58.9 1.0
CD2 A:HIS232 4.8 60.0 1.0
O A:GLU229 4.9 52.6 1.0
C A:GLU229 5.0 52.8 1.0
CA A:GLU196 5.0 56.1 1.0

Iron binding site 3 out of 12 in 4v0j

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Iron binding site 3 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe364

b:66.9
occ:1.00
 OE2 B:GLU105 2.1 57.1 1.0
OE1 B:GLU143 2.1 64.1 1.0
OE2 B:GLU229 2.2 61.6 1.0
ND1 B:HIS146 2.3 70.8 1.0
OE1 B:GLU105 2.5 54.4 1.0
CD B:GLU105 2.6 55.5 1.0
CE1 B:HIS146 3.1 72.1 1.0
CD B:GLU229 3.1 54.4 1.0
CD B:GLU143 3.2 58.2 1.0
OE1 B:GLU229 3.3 60.8 1.0
CG B:HIS146 3.4 76.4 1.0
OE2 B:GLU143 3.8 64.1 1.0
CB B:HIS146 3.9 69.4 1.0
FE B:FE2365 4.0 69.5 1.0
CG B:GLU105 4.1 53.1 1.0
NE2 B:HIS146 4.3 76.9 1.0
NE2 B:HIS203 4.3 79.0 1.0
CG B:GLU229 4.5 52.9 1.0
CD2 B:HIS146 4.5 78.0 1.0
CG B:GLU143 4.5 53.1 1.0
CG2 B:ILE225 4.5 46.0 1.0
CB B:GLU143 4.6 50.9 1.0
CA B:GLU143 4.8 49.4 1.0
O B:GLU143 4.8 54.9 1.0
CD2 B:HIS203 4.8 80.1 1.0

Iron binding site 4 out of 12 in 4v0j

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Iron binding site 4 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Fe365

b:69.5
occ:1.00
 OE2 B:GLU143 1.8 64.1 1.0
OE1 B:GLU229 1.9 60.8 1.0
OE1 B:GLU196 2.1 46.3 1.0
OE2 B:GLU196 2.5 48.9 1.0
ND1 B:HIS232 2.6 63.7 1.0
CD B:GLU196 2.7 45.9 1.0
CD B:GLU143 2.8 58.2 1.0
CD B:GLU229 3.1 54.4 1.0
OE1 B:GLU143 3.3 64.1 1.0
CE1 B:HIS232 3.5 71.4 1.0
CG B:HIS232 3.7 64.6 1.0
OE2 B:GLU229 3.7 61.6 1.0
NE1 B:TRP139 3.9 52.6 1.0
FE B:FE2364 4.0 66.9 1.0
CB B:HIS232 4.0 64.8 1.0
CA B:GLU229 4.0 59.0 1.0
CG B:GLU196 4.1 49.2 1.0
CG B:GLU143 4.2 53.1 1.0
CB B:GLU229 4.2 54.3 1.0
CG B:GLU229 4.2 52.9 1.0
CZ2 B:TRP139 4.4 62.9 1.0
CE2 B:TRP139 4.4 58.3 1.0
OG1 B:THR199 4.4 69.4 1.0
CE1 B:HIS146 4.6 72.1 1.0
NE2 B:HIS232 4.6 75.4 1.0
N B:GLU229 4.8 59.2 1.0
CD2 B:HIS232 4.8 65.7 1.0
CB B:GLU196 4.8 53.2 1.0
CD1 B:TRP139 4.9 54.4 1.0
O B:GLU229 4.9 64.8 1.0

Iron binding site 5 out of 12 in 4v0j

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Iron binding site 5 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe364

b:61.9
occ:1.00
 OE1 C:GLU143 2.0 48.9 1.0
OE2 C:GLU229 2.4 59.2 1.0
OE2 C:GLU105 2.4 65.2 1.0
OE1 C:GLU105 2.7 49.6 1.0
CD C:GLU105 2.8 50.9 1.0
ND1 C:HIS146 2.9 34.8 1.0
CD C:GLU143 3.0 42.2 1.0
CD C:GLU229 3.1 56.9 1.0
OE1 C:GLU229 3.2 56.3 1.0
OE2 C:GLU143 3.4 46.8 1.0
CE1 C:HIS146 3.5 36.0 1.0
FE C:FE2365 3.6 59.4 1.0
CG C:HIS146 4.1 34.0 1.0
CG C:GLU143 4.3 40.4 1.0
CG C:GLU105 4.3 46.7 1.0
CB C:HIS146 4.5 34.5 1.0
CB C:GLU143 4.5 37.5 1.0
CG C:GLU229 4.6 55.5 1.0
NE2 C:HIS203 4.6 56.5 1.0
NE2 C:HIS146 4.8 35.6 1.0
OG1 C:THR199 4.8 55.5 1.0
CA C:GLU143 4.9 35.3 1.0
CB C:THR199 4.9 50.7 1.0

Iron binding site 6 out of 12 in 4v0j

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Iron binding site 6 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe365

b:59.4
occ:1.00
 OE1 C:GLU196 1.8 53.7 1.0
OE1 C:GLU229 2.0 56.3 1.0
OE2 C:GLU143 2.0 46.8 1.0
OE2 C:GLU196 2.5 51.0 1.0
CD C:GLU196 2.5 46.8 1.0
ND1 C:HIS232 2.9 30.6 1.0
CD C:GLU229 3.1 56.9 1.0
CD C:GLU143 3.1 42.2 1.0
OE1 C:GLU143 3.5 48.9 1.0
FE C:FE2364 3.6 61.9 1.0
OE2 C:GLU229 3.7 59.2 1.0
CE1 C:HIS232 3.8 29.7 1.0
CG C:HIS232 3.8 32.6 1.0
CG C:GLU196 3.9 44.8 1.0
CA C:GLU229 4.0 49.8 1.0
CB C:HIS232 4.0 33.6 1.0
NE1 C:TRP139 4.0 53.0 1.0
CB C:GLU229 4.1 51.7 1.0
CG C:GLU229 4.1 55.5 1.0
OG1 C:THR199 4.1 55.5 1.0
CG C:GLU143 4.4 40.4 1.0
CZ2 C:TRP139 4.4 53.1 1.0
CE2 C:TRP139 4.5 51.8 1.0
CB C:GLU196 4.6 44.1 1.0
CA C:GLU196 4.7 47.2 1.0
O C:GLU229 4.8 47.8 1.0
N C:GLU229 4.9 48.9 1.0
CE1 C:HIS146 4.9 36.0 1.0
NE2 C:HIS232 4.9 27.7 1.0
C C:GLU229 5.0 51.4 1.0
CD2 C:HIS232 5.0 27.9 1.0

Iron binding site 7 out of 12 in 4v0j

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Iron binding site 7 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Fe364

b:50.5
occ:1.00
 OE2 D:GLU105 2.2 28.8 1.0
OE1 D:GLU143 2.2 37.3 1.0
OE2 D:GLU229 2.3 35.1 1.0
OE1 D:GLU105 2.5 30.6 1.0
ND1 D:HIS146 2.7 43.3 1.0
CD D:GLU105 2.7 30.4 1.0
CD D:GLU143 3.1 38.8 1.0
CD D:GLU229 3.1 31.8 1.0
O D:HOH2006 3.2 13.8 1.0
OE1 D:GLU229 3.2 31.7 1.0
CE1 D:HIS146 3.4 44.1 1.0
OE2 D:GLU143 3.4 36.8 1.0
CG D:HIS146 3.8 45.8 1.0
FE D:FE2365 3.9 46.5 1.0
CG D:GLU105 4.2 28.0 1.0
CB D:HIS146 4.2 43.4 1.0
O D:HOH2003 4.3 26.9 1.0
CG D:GLU143 4.4 41.8 1.0
NE2 D:HIS203 4.4 32.5 1.0
CG D:GLU229 4.5 31.1 1.0
CB D:GLU143 4.6 40.2 1.0
NE2 D:HIS146 4.6 42.6 1.0
CA D:GLU143 4.8 39.7 1.0
CD2 D:HIS146 4.8 46.7 1.0
CG2 D:ILE225 4.9 37.6 1.0
OG1 D:THR199 4.9 42.2 1.0
CD2 D:HIS203 4.9 29.4 1.0

Iron binding site 8 out of 12 in 4v0j

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Iron binding site 8 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 8 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Fe365

b:46.5
occ:1.00
 OE1 D:GLU196 1.9 33.5 1.0
OE1 D:GLU229 2.0 31.7 1.0
OE2 D:GLU143 2.2 36.8 1.0
OE2 D:GLU196 2.5 31.0 1.0
CD D:GLU196 2.5 33.8 1.0
O D:HOH2006 2.5 13.8 1.0
ND1 D:HIS232 2.6 45.3 1.0
CD D:GLU143 3.0 38.8 1.0
CD D:GLU229 3.1 31.8 1.0
OE1 D:GLU143 3.2 37.3 1.0
CE1 D:HIS232 3.5 45.4 1.0
CG D:HIS232 3.6 44.7 1.0
CB D:HIS232 3.8 44.1 1.0
OE2 D:GLU229 3.8 35.1 1.0
NE1 D:TRP139 3.9 36.3 1.0
FE D:FE2364 3.9 50.5 1.0
CA D:GLU229 3.9 34.3 1.0
CG D:GLU196 4.0 35.0 1.0
O D:HOH2008 4.1 40.6 1.0
CB D:GLU229 4.1 33.5 1.0
CG D:GLU229 4.2 31.1 1.0
CG D:GLU143 4.3 41.8 1.0
OG1 D:THR199 4.4 42.2 1.0
CZ2 D:TRP139 4.4 39.3 1.0
CE2 D:TRP139 4.4 38.6 1.0
NE2 D:HIS232 4.6 44.4 1.0
CB D:GLU196 4.7 35.2 1.0
CD2 D:HIS232 4.7 46.6 1.0
CE1 D:HIS146 4.8 44.1 1.0
N D:GLU229 4.8 30.2 1.0
O D:GLU229 4.8 43.1 1.0
CA D:GLU196 4.9 34.3 1.0
C D:GLU229 4.9 38.0 1.0
CD1 D:TRP139 4.9 39.3 1.0

Iron binding site 9 out of 12 in 4v0j

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Iron binding site 9 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 9 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Fe364

b:56.3
occ:1.00
 OE1 E:GLU143 2.1 36.7 1.0
OE2 E:GLU105 2.2 35.1 1.0
OE2 E:GLU229 2.2 38.7 1.0
OE1 E:GLU105 2.6 35.0 1.0
CD E:GLU105 2.7 34.1 1.0
ND1 E:HIS146 2.7 30.4 1.0
O E:HOH2002 2.9 18.3 1.0
CD E:GLU229 3.1 38.5 1.0
CD E:GLU143 3.1 34.1 1.0
OE1 E:GLU229 3.2 33.9 1.0
CE1 E:HIS146 3.4 32.8 1.0
OE2 E:GLU143 3.6 33.4 1.0
CG E:HIS146 3.9 32.0 1.0
FE E:FE2365 3.9 56.0 1.0
CG E:GLU105 4.2 35.6 1.0
CB E:HIS146 4.3 32.2 1.0
CG E:GLU143 4.4 34.5 1.0
NE2 E:HIS203 4.4 37.6 1.0
CG E:GLU229 4.5 39.3 1.0
CB E:GLU143 4.6 33.5 1.0
NE2 E:HIS146 4.6 32.5 1.0
OG1 E:THR199 4.9 42.4 1.0
CD2 E:HIS146 4.9 31.2 1.0
CA E:GLU143 4.9 30.0 1.0
CG2 E:ILE225 4.9 32.8 1.0
CD2 E:HIS203 4.9 37.8 1.0
CB E:THR199 5.0 40.6 1.0

Iron binding site 10 out of 12 in 4v0j

Go back to Iron Binding Sites List in 4v0j
Iron binding site 10 out of 12 in the The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 10 of The Channel-Block SER202GLU, THR104LYS Double Mutant of Stearoyl-Acp-Desaturase From Castor Bean (Ricinus Communis) within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Fe365

b:56.0
occ:1.00
 OE1 E:GLU196 1.9 44.0 1.0
OE2 E:GLU143 1.9 33.4 1.0
OE1 E:GLU229 2.1 33.9 1.0
OE2 E:GLU196 2.3 36.3 1.0
O E:HOH2002 2.4 18.3 1.0
CD E:GLU196 2.4 36.5 1.0
ND1 E:HIS232 2.8 45.0 1.0
CD E:GLU143 3.0 34.1 1.0
CD E:GLU229 3.2 38.5 1.0
OE1 E:GLU143 3.5 36.7 1.0
CE1 E:HIS232 3.7 43.6 1.0
CG E:HIS232 3.7 43.7 1.0
NE1 E:TRP139 3.8 37.7 1.0
FE E:FE2364 3.9 56.3 1.0
CG E:GLU196 3.9 35.3 1.0
CB E:HIS232 3.9 44.2 1.0
OE2 E:GLU229 3.9 38.7 1.0
O E:HOH2007 4.0 43.7 1.0
CA E:GLU229 4.1 36.8 1.0
CB E:GLU229 4.2 38.3 1.0
CZ2 E:TRP139 4.3 48.8 1.0
CG E:GLU143 4.3 34.5 1.0
CG E:GLU229 4.3 39.3 1.0
OG1 E:THR199 4.3 42.4 1.0
CE2 E:TRP139 4.4 41.4 1.0
CB E:GLU196 4.6 34.2 1.0
CA E:GLU196 4.8 35.0 1.0
NE2 E:HIS232 4.8 41.0 1.0
CD2 E:HIS232 4.9 42.4 1.0
O E:GLU229 4.9 37.5 1.0
CE1 E:HIS146 4.9 32.8 1.0
N E:GLU229 4.9 32.2 1.0
CD1 E:TRP139 4.9 36.4 1.0

Reference:

Q.Liu, J.Chai, M.Moche, J.Guy, Y.Lindqvist, J.Shanklin. Half-of-the-Sites Reactivity of the Castor DELTA9-18:0-Acp Desaturase. Plant Physiol. V. 169 432 2015.
ISSN: ISSN 0032-0889
PubMed: 26224800
DOI: 10.1104/PP.15.00622
Page generated: Mon Aug 5 14:10:01 2024

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