Iron in PDB 4z5p: Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution

The structure of Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution, PDB code: 4z5p was solved by M.Ma, J.Lohman, J.Rudolf, M.D.Miller, H.Cao, J.Osipiuk, G.Babnigg, G.N.Phillips Jr., A.Joachimiak, B.Shen, Midwest Center For Structuralgenomics (Mcsg), Enzyme Discovery For Natural Product Biosynthesis(Natpro), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.36 / 1.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.759, 75.454, 93.858, 90.00, 98.91, 90.00
R / Rfree (%)	19.1 / 21.7

The binding sites of Iron atom in the Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution (pdb code 4z5p). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution, PDB code: 4z5p:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe401 b:24.3 occ:1.00 FE A:HEM401 0.0 24.3 1.0 ND A:HEM401 1.9 26.0 1.0 NA A:HEM401 2.0 25.4 1.0 NB A:HEM401 2.1 25.4 1.0 NC A:HEM401 2.1 25.2 1.0 O A:HOH403 2.3 31.3 1.0 SG A:CYS348 2.4 25.6 1.0 C4D A:HEM401 2.9 26.9 1.0 C1D A:HEM401 3.0 25.0 1.0 C1A A:HEM401 3.0 25.2 1.0 C4B A:HEM401 3.0 24.3 1.0 C4A A:HEM401 3.0 25.1 1.0 C1C A:HEM401 3.1 24.7 1.0 C1B A:HEM401 3.1 22.5 1.0 C4C A:HEM401 3.1 24.5 1.0 CHA A:HEM401 3.4 26.7 1.0 CHC A:HEM401 3.4 24.0 1.0 CHB A:HEM401 3.4 23.9 1.0 CHD A:HEM401 3.4 23.5 1.0 CB A:CYS348 3.5 23.0 1.0 CA A:CYS348 4.1 22.3 1.0 O A:ALA236 4.1 26.6 1.0 C3D A:HEM401 4.2 27.5 1.0 C2A A:HEM401 4.2 29.6 1.0 C2D A:HEM401 4.2 27.7 1.0 C3A A:HEM401 4.2 27.2 1.0 C3B A:HEM401 4.3 23.7 1.0 C2C A:HEM401 4.3 24.1 1.0 C2B A:HEM401 4.3 23.5 1.0 C3C A:HEM401 4.3 25.3 1.0 CB A:ALA236 4.7 23.5 1.0 C A:CYS348 4.8 22.1 1.0 C A:ALA236 4.8 25.2 1.0 N A:LEU349 4.8 23.7 1.0 N A:GLY350 4.9 21.0 1.0

Iron binding site 2 out of 2 in the Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 at 1.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe401 b:21.4 occ:1.00 FE B:HEM401 0.0 21.4 1.0 ND B:HEM401 1.9 20.5 1.0 NA B:HEM401 2.0 21.8 1.0 NB B:HEM401 2.1 21.1 1.0 NC B:HEM401 2.1 20.8 1.0 SG B:CYS348 2.4 21.4 1.0 O B:HOH403 2.5 31.0 1.0 C1D B:HEM401 3.0 22.2 1.0 C4D B:HEM401 3.0 20.7 1.0 C4A B:HEM401 3.0 23.3 1.0 C1B B:HEM401 3.0 21.8 1.0 C1A B:HEM401 3.0 23.4 1.0 C4B B:HEM401 3.0 22.8 1.0 C4C B:HEM401 3.1 20.8 1.0 C1C B:HEM401 3.1 21.7 1.0 CHB B:HEM401 3.4 21.9 1.0 CHD B:HEM401 3.4 20.2 1.0 CHA B:HEM401 3.4 23.1 1.0 CB B:CYS348 3.5 19.8 1.0 CHC B:HEM401 3.5 21.9 1.0 CA B:CYS348 4.1 20.4 1.0 O B:ALA236 4.1 24.9 1.0 C3A B:HEM401 4.2 24.9 1.0 C2A B:HEM401 4.2 23.8 1.0 C3D B:HEM401 4.2 23.6 1.0 C2D B:HEM401 4.3 22.4 1.0 C2B B:HEM401 4.3 24.7 1.0 C3C B:HEM401 4.3 21.9 1.0 C3B B:HEM401 4.3 23.0 1.0 C2C B:HEM401 4.3 21.3 1.0 CB B:ALA236 4.6 25.5 1.0 C B:CYS348 4.8 20.7 1.0 C B:ALA236 4.8 23.9 1.0 N B:LEU349 4.8 20.9 1.0 N B:GLY350 4.8 20.4 1.0 CD1 B:PHE341 4.9 18.4 1.0

M.Ma, J.Lohman, J.Rudolf, M.D.Miller, H.Cao, J.Osipiuk, G.Babnigg, G.N.Phillips Jr., A.Joachimiak, B.Shen. Crystal Structure of the Lnma Cytochrome P450 Hydroxylase From the Leinamycin Biosynthetic Pathway of Streptomyces Atroolivaceus S-140 To Be Published.