Iron in PDB 4z82: Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1

All present enzymatic activity of Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1:
1.13.11.20;

The structure of Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1, PDB code: 4z82 was solved by M.Fellner, E.P.Tchesnokov, E.Siakkou, M.T.Rutledge, M.Kanitz, G.N.L.Jameson, S.M.Wilbanks, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.88 / 1.70
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	58.020, 58.020, 122.230, 90.00, 90.00, 90.00
R / Rfree (%)	17.8 / 20.6

The binding sites of Iron atom in the Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1 (pdb code 4z82). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1, PDB code: 4z82:

Iron binding site 1 out of 1 in the Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cysteine Bound Rat Cysteine Dioxygenase C164S Variant at pH 8.1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe301 b:21.1 occ:1.00 NE2 A:HIS86 2.0 18.3 1.0 NE2 A:HIS88 2.2 22.0 1.0 O A:HOH401 2.3 28.1 0.6 N A:CYS302 2.3 26.4 0.9 NE2 A:HIS140 2.4 21.7 1.0 SG A:CYS302 2.4 24.6 0.9 CD2 A:HIS86 3.0 22.2 1.0 CE1 A:HIS86 3.0 16.3 1.0 CE1 A:HIS88 3.1 21.0 1.0 CA A:CYS302 3.1 23.6 0.9 CD2 A:HIS88 3.2 20.8 1.0 CD2 A:HIS140 3.3 23.3 1.0 CB A:CYS302 3.3 20.4 0.9 CE1 A:HIS140 3.4 22.4 1.0 ND1 A:HIS86 4.1 20.8 1.0 CG A:HIS86 4.2 20.5 1.0 ND1 A:HIS88 4.2 22.5 1.0 CG A:HIS88 4.3 21.8 1.0 CG A:HIS140 4.4 19.8 1.0 ND1 A:HIS140 4.4 20.3 1.0 OH A:TYR157 4.5 28.6 1.0 C A:CYS302 4.5 30.8 0.9 O A:HOH559 4.6 26.5 1.0 SG A:CYS93 4.7 27.9 1.0 CG2 A:VAL142 4.8 24.6 1.0 O A:CYS302 4.9 27.1 0.9 CD2 A:LEU95 5.0 24.6 0.4

M.Fellner, E.Siakkou, A.S.Faponle, E.P.Tchesnokov, S.P.De Visser, S.M.Wilbanks, G.N.Jameson. Influence of Cysteine 164 on Active Site Structure in Rat Cysteine Dioxygenase. J.Biol.Inorg.Chem. V. 21 501 2016.
ISSN: ESSN 1432-1327
PubMed: 27193596
DOI: 10.1007/S00775-016-1360-0