Iron in PDB 4zn3: Crystal Structure of MJSPT4:SPT5 Complex Conformation B

The structure of Crystal Structure of MJSPT4:SPT5 Complex Conformation B, PDB code: 4zn3 was solved by G.R.Guo, H.H.Zhou, Y.X.Gao, Z.L.Zhu, L.W.Niu, M.K.Teng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	45.395, 88.943, 91.955, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 25.6

The structure of Crystal Structure of MJSPT4:SPT5 Complex Conformation B also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Iron atom in the Crystal Structure of MJSPT4:SPT5 Complex Conformation B (pdb code 4zn3). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of MJSPT4:SPT5 Complex Conformation B, PDB code: 4zn3:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of MJSPT4:SPT5 Complex Conformation B


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of MJSPT4:SPT5 Complex Conformation B within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe203 b:49.4 occ:1.00 O B:HOH204 2.1 57.1 1.0 NE2 B:HIS-3 2.3 57.2 1.0 NE2 A:HIS145 2.3 58.0 1.0 OE2 A:GLU90 2.4 51.7 1.0 OE1 A:GLU90 2.5 54.9 1.0 ND1 B:HIS-5 2.5 68.2 1.0 CD A:GLU90 2.8 53.1 1.0 CG B:HIS-5 3.1 65.9 1.0 CD2 B:HIS-3 3.1 57.3 1.0 CD2 A:HIS145 3.3 58.5 1.0 CE1 A:HIS145 3.3 58.9 1.0 CE1 B:HIS-3 3.3 58.5 1.0 CE1 B:HIS-5 3.3 70.0 1.0 CB B:HIS-5 3.4 63.1 1.0 CD2 B:HIS-5 4.0 68.5 1.0 NE2 B:HIS-5 4.1 70.6 1.0 CG A:GLU90 4.3 54.8 1.0 CG B:HIS-3 4.3 57.3 1.0 ND1 B:HIS-3 4.4 57.9 1.0 CE A:LYS144 4.4 57.8 1.0 ND1 A:HIS145 4.4 57.7 1.0 CG A:HIS145 4.4 57.6 1.0 NZ A:LYS144 4.5 58.0 1.0 O A:HOH319 4.9 65.4 1.0 CB A:GLU90 4.9 54.6 1.0 CA B:HIS-5 4.9 62.0 1.0

Iron binding site 2 out of 2 in the Crystal Structure of MJSPT4:SPT5 Complex Conformation B


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of MJSPT4:SPT5 Complex Conformation B within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe204 b:53.4 occ:1.00 O A:ASP147 1.9 65.1 1.0 NE2 B:HIS-4 2.2 60.1 1.0 ND1 B:HIS-6 2.2 59.8 1.0 CE1 B:HIS-4 2.8 61.0 1.0 C A:ASP147 2.9 62.9 1.0 CE1 B:HIS-6 3.0 60.6 1.0 CD2 B:HIS-4 3.3 60.5 1.0 CG B:HIS-6 3.3 64.4 1.0 CB B:HIS-6 3.7 66.3 1.0 ND1 B:HIS-4 3.9 60.5 1.0 NE2 B:HIS-6 4.1 63.5 1.0 CG B:HIS-4 4.1 59.4 1.0 CA B:HIS-6 4.2 68.1 1.0 CA A:ASP147 4.3 65.0 1.0 CD2 B:HIS-6 4.3 63.3 1.0 OD1 A:ASP147 4.4 63.3 1.0 CB A:ASP147 4.6 62.6 1.0 CG A:ASP147 4.7 63.1 1.0 C B:HIS-6 5.0 67.0 1.0

G.R.Guo, Y.X.Gao, Z.L.Zhu, D.Zhao, Z.Liu, H.H.Zhou, L.W.Niu, M.K.Teng. Structural and Biochemical Insights Into the Dna-Binding Mode of MJSPT4P:SPT5 Complex at the Exit Tunnel of Rnapii J.Struct.Biol. V. 192 418 2015.
ISSN: ESSN 1095-8657
PubMed: 26433031
DOI: 10.1016/J.JSB.2015.09.023