Iron in PDB 5exr: Crystal Structure of Human Primosome
Enzymatic activity of Crystal Structure of Human Primosome
All present enzymatic activity of Crystal Structure of Human Primosome:
2.7.7.7;
Protein crystallography data
The structure of Crystal Structure of Human Primosome, PDB code: 5exr
was solved by
T.H.Tahirov,
A.G.Baranovskiy,
N.D.Babayeva,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
39.94 /
3.60
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
113.096,
210.164,
172.565,
90.00,
93.56,
90.00
|
R / Rfree (%)
|
26.8 /
32.6
|
Other elements in 5exr:
The structure of Crystal Structure of Human Primosome also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Crystal Structure of Human Primosome
(pdb code 5exr). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 8 binding sites of Iron where determined in the
Crystal Structure of Human Primosome, PDB code: 5exr:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Iron binding site 1 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 1 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:1.1
occ:1.00
|
FE1
|
B:SF4601
|
0.0
|
1.1
|
1.0
|
S4
|
B:SF4601
|
2.1
|
1.1
|
1.0
|
S2
|
B:SF4601
|
2.1
|
2.4
|
1.0
|
S3
|
B:SF4601
|
2.2
|
1.1
|
1.0
|
SG
|
B:CYS367
|
2.4
|
7.8
|
1.0
|
FE2
|
B:SF4601
|
2.8
|
1.1
|
1.0
|
FE3
|
B:SF4601
|
2.9
|
1.1
|
1.0
|
CB
|
B:CYS367
|
3.0
|
27.1
|
1.0
|
FE4
|
B:SF4601
|
3.1
|
9.2
|
1.0
|
CG
|
B:GLN421
|
3.3
|
1.2
|
1.0
|
S1
|
B:SF4601
|
3.6
|
1.1
|
1.0
|
CD
|
B:GLN421
|
3.7
|
1.1
|
1.0
|
CA
|
B:CYS367
|
3.9
|
26.2
|
1.0
|
OE1
|
B:GLN421
|
4.1
|
2.8
|
1.0
|
NE2
|
B:GLN421
|
4.4
|
1.1
|
1.0
|
CB
|
B:GLN421
|
4.4
|
13.8
|
1.0
|
CD
|
B:PRO444
|
4.4
|
23.4
|
1.0
|
CG
|
B:PRO444
|
4.5
|
18.2
|
1.0
|
C
|
B:CYS367
|
4.8
|
22.6
|
1.0
|
N
|
B:PRO444
|
4.9
|
21.2
|
1.0
|
N
|
B:CYS367
|
5.0
|
25.3
|
1.0
|
CA
|
B:GLN421
|
5.0
|
15.3
|
1.0
|
|
Iron binding site 2 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 2 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:1.1
occ:1.00
|
FE2
|
B:SF4601
|
0.0
|
1.1
|
1.0
|
S3
|
B:SF4601
|
2.1
|
1.1
|
1.0
|
S4
|
B:SF4601
|
2.1
|
1.1
|
1.0
|
S1
|
B:SF4601
|
2.2
|
1.1
|
1.0
|
FE1
|
B:SF4601
|
2.8
|
1.1
|
1.0
|
FE3
|
B:SF4601
|
2.8
|
1.1
|
1.0
|
FE4
|
B:SF4601
|
3.0
|
9.2
|
1.0
|
SG
|
B:CYS384
|
3.2
|
56.0
|
1.0
|
CB
|
B:CYS384
|
3.2
|
51.3
|
1.0
|
S2
|
B:SF4601
|
3.4
|
2.4
|
1.0
|
SG
|
B:CYS287
|
3.8
|
1.1
|
1.0
|
CA
|
B:CYS384
|
3.8
|
47.3
|
1.0
|
CB
|
B:CYS287
|
4.1
|
14.2
|
1.0
|
CD
|
B:PRO385
|
4.4
|
27.4
|
1.0
|
N
|
B:CYS384
|
4.8
|
51.8
|
1.0
|
CG2
|
B:ILE370
|
4.8
|
1.1
|
1.0
|
C
|
B:CYS384
|
4.8
|
40.9
|
1.0
|
CG1
|
B:ILE371
|
4.8
|
29.8
|
1.0
|
N
|
B:PRO385
|
5.0
|
33.7
|
1.0
|
SG
|
B:CYS367
|
5.0
|
7.8
|
1.0
|
CG
|
B:GLN421
|
5.0
|
1.2
|
1.0
|
|
Iron binding site 3 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 3 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:1.1
occ:1.00
|
FE3
|
B:SF4601
|
0.0
|
1.1
|
1.0
|
S4
|
B:SF4601
|
2.0
|
1.1
|
1.0
|
S2
|
B:SF4601
|
2.1
|
2.4
|
1.0
|
S1
|
B:SF4601
|
2.2
|
1.1
|
1.0
|
FE2
|
B:SF4601
|
2.8
|
1.1
|
1.0
|
FE1
|
B:SF4601
|
2.9
|
1.1
|
1.0
|
CB
|
B:CYS424
|
3.0
|
46.9
|
1.0
|
SG
|
B:CYS424
|
3.1
|
55.3
|
1.0
|
FE4
|
B:SF4601
|
3.2
|
9.2
|
1.0
|
S3
|
B:SF4601
|
3.7
|
1.1
|
1.0
|
CD2
|
B:PHE386
|
3.9
|
46.8
|
1.0
|
CG
|
B:GLN421
|
4.1
|
1.2
|
1.0
|
CE2
|
B:PHE386
|
4.1
|
43.8
|
1.0
|
CA
|
B:GLN421
|
4.2
|
15.3
|
1.0
|
CA
|
B:CYS424
|
4.3
|
49.6
|
1.0
|
SG
|
B:CYS384
|
4.4
|
56.0
|
1.0
|
CB
|
B:GLN421
|
4.5
|
13.8
|
1.0
|
CD
|
B:PRO286
|
4.6
|
28.9
|
1.0
|
O
|
B:TYR420
|
4.7
|
18.7
|
1.0
|
N
|
B:CYS424
|
4.8
|
44.4
|
1.0
|
O
|
B:GLN421
|
4.9
|
14.3
|
1.0
|
SG
|
B:CYS287
|
4.9
|
1.1
|
1.0
|
N
|
B:GLN421
|
4.9
|
18.3
|
1.0
|
|
Iron binding site 4 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 4 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:9.2
occ:1.00
|
FE4
|
B:SF4601
|
0.0
|
9.2
|
1.0
|
S3
|
B:SF4601
|
2.1
|
1.1
|
1.0
|
S1
|
B:SF4601
|
2.1
|
1.1
|
1.0
|
S2
|
B:SF4601
|
2.1
|
2.4
|
1.0
|
SG
|
B:CYS287
|
2.2
|
1.1
|
1.0
|
FE2
|
B:SF4601
|
3.0
|
1.1
|
1.0
|
FE1
|
B:SF4601
|
3.1
|
1.1
|
1.0
|
CG
|
B:PRO285
|
3.1
|
25.1
|
1.0
|
FE3
|
B:SF4601
|
3.2
|
1.1
|
1.0
|
CB
|
B:PRO285
|
3.5
|
26.4
|
1.0
|
CG
|
B:PRO444
|
3.5
|
18.2
|
1.0
|
CB
|
B:CYS287
|
3.8
|
14.2
|
1.0
|
S4
|
B:SF4601
|
4.0
|
1.1
|
1.0
|
CB
|
B:PRO444
|
4.3
|
15.8
|
1.0
|
CD
|
B:PRO444
|
4.4
|
23.4
|
1.0
|
CA
|
B:PRO444
|
4.4
|
18.8
|
1.0
|
N
|
B:CYS287
|
4.4
|
19.6
|
1.0
|
SG
|
B:CYS367
|
4.5
|
7.8
|
1.0
|
SG
|
B:CYS424
|
4.5
|
55.3
|
1.0
|
CD
|
B:PRO286
|
4.6
|
28.9
|
1.0
|
CD
|
B:PRO285
|
4.6
|
22.5
|
1.0
|
N
|
B:PRO444
|
4.7
|
21.2
|
1.0
|
CA
|
B:CYS287
|
4.7
|
18.4
|
1.0
|
CA
|
B:PRO285
|
4.8
|
23.2
|
1.0
|
N
|
B:PRO286
|
4.9
|
29.4
|
1.0
|
CE
|
B:MET288
|
5.0
|
19.0
|
1.0
|
C
|
B:PRO285
|
5.0
|
27.6
|
1.0
|
|
Iron binding site 5 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 5 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Fe601
b:18.3
occ:1.00
|
FE1
|
F:SF4601
|
0.0
|
18.3
|
1.0
|
S4
|
F:SF4601
|
2.1
|
1.1
|
1.0
|
S2
|
F:SF4601
|
2.1
|
4.5
|
1.0
|
S3
|
F:SF4601
|
2.2
|
1.1
|
1.0
|
SG
|
F:CYS367
|
2.4
|
9.6
|
1.0
|
FE2
|
F:SF4601
|
2.8
|
1.1
|
1.0
|
CB
|
F:CYS367
|
2.9
|
23.4
|
1.0
|
FE3
|
F:SF4601
|
2.9
|
8.8
|
1.0
|
FE4
|
F:SF4601
|
3.2
|
1.1
|
1.0
|
CA
|
F:CYS367
|
3.6
|
22.1
|
1.0
|
S1
|
F:SF4601
|
3.7
|
8.0
|
1.0
|
NE2
|
F:GLN421
|
4.1
|
16.1
|
1.0
|
CG
|
F:GLN421
|
4.4
|
15.2
|
1.0
|
C
|
F:CYS367
|
4.5
|
21.9
|
1.0
|
CD
|
F:PRO444
|
4.5
|
16.7
|
1.0
|
CB
|
F:GLN421
|
4.6
|
22.7
|
1.0
|
CG
|
F:PRO444
|
4.6
|
9.2
|
1.0
|
CD
|
F:GLN421
|
4.6
|
10.3
|
1.0
|
O
|
F:CYS367
|
4.7
|
25.7
|
1.0
|
N
|
F:CYS367
|
4.8
|
20.2
|
1.0
|
SG
|
F:CYS287
|
4.9
|
1.1
|
1.0
|
CG1
|
F:ILE371
|
5.0
|
7.6
|
1.0
|
|
Iron binding site 6 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 6 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Fe601
b:1.1
occ:1.00
|
FE2
|
F:SF4601
|
0.0
|
1.1
|
1.0
|
S3
|
F:SF4601
|
2.2
|
1.1
|
1.0
|
S4
|
F:SF4601
|
2.2
|
1.1
|
1.0
|
S1
|
F:SF4601
|
2.2
|
8.0
|
1.0
|
FE1
|
F:SF4601
|
2.8
|
18.3
|
1.0
|
FE3
|
F:SF4601
|
2.9
|
8.8
|
1.0
|
FE4
|
F:SF4601
|
3.1
|
1.1
|
1.0
|
CB
|
F:CYS384
|
3.3
|
51.3
|
1.0
|
SG
|
F:CYS384
|
3.4
|
54.4
|
1.0
|
S2
|
F:SF4601
|
3.4
|
4.5
|
1.0
|
SG
|
F:CYS287
|
3.6
|
1.1
|
1.0
|
CA
|
F:CYS384
|
3.9
|
41.7
|
1.0
|
CD
|
F:PRO385
|
3.9
|
1.1
|
1.0
|
CB
|
F:CYS287
|
3.9
|
27.9
|
1.0
|
CG
|
F:PRO385
|
4.7
|
9.4
|
1.0
|
N
|
F:PRO385
|
4.7
|
17.7
|
1.0
|
N
|
F:CYS287
|
4.7
|
35.0
|
1.0
|
C
|
F:CYS384
|
4.7
|
26.1
|
1.0
|
CD2
|
F:PHE386
|
4.8
|
37.3
|
1.0
|
CA
|
F:CYS287
|
4.9
|
32.3
|
1.0
|
N
|
F:CYS384
|
5.0
|
44.2
|
1.0
|
|
Iron binding site 7 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 7 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Fe601
b:8.8
occ:1.00
|
FE3
|
F:SF4601
|
0.0
|
8.8
|
1.0
|
S4
|
F:SF4601
|
2.0
|
1.1
|
1.0
|
S2
|
F:SF4601
|
2.2
|
4.5
|
1.0
|
S1
|
F:SF4601
|
2.2
|
8.0
|
1.0
|
CB
|
F:CYS424
|
2.8
|
48.1
|
1.0
|
FE2
|
F:SF4601
|
2.9
|
1.1
|
1.0
|
SG
|
F:CYS424
|
2.9
|
43.8
|
1.0
|
FE1
|
F:SF4601
|
2.9
|
18.3
|
1.0
|
FE4
|
F:SF4601
|
3.3
|
1.1
|
1.0
|
S3
|
F:SF4601
|
3.7
|
1.1
|
1.0
|
CA
|
F:GLN421
|
4.2
|
22.6
|
1.0
|
CA
|
F:CYS424
|
4.2
|
49.8
|
1.0
|
CD2
|
F:PHE386
|
4.3
|
37.3
|
1.0
|
CE2
|
F:PHE386
|
4.4
|
34.5
|
1.0
|
CB
|
F:GLN421
|
4.4
|
22.7
|
1.0
|
SG
|
F:CYS367
|
4.5
|
9.6
|
1.0
|
O
|
F:GLN421
|
4.7
|
31.4
|
1.0
|
CD
|
F:PRO286
|
4.8
|
44.2
|
1.0
|
N
|
F:CYS424
|
4.9
|
52.7
|
1.0
|
CB
|
F:PRO285
|
4.9
|
35.6
|
1.0
|
CD2
|
F:LEU441
|
4.9
|
41.3
|
1.0
|
SG
|
F:CYS287
|
4.9
|
1.1
|
1.0
|
C
|
F:GLN421
|
5.0
|
31.6
|
1.0
|
|
Iron binding site 8 out
of 8 in 5exr
Go back to
Iron Binding Sites List in 5exr
Iron binding site 8 out
of 8 in the Crystal Structure of Human Primosome
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of Crystal Structure of Human Primosome within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Fe601
b:1.1
occ:1.00
|
FE4
|
F:SF4601
|
0.0
|
1.1
|
1.0
|
S3
|
F:SF4601
|
2.1
|
1.1
|
1.0
|
S1
|
F:SF4601
|
2.1
|
8.0
|
1.0
|
S2
|
F:SF4601
|
2.2
|
4.5
|
1.0
|
SG
|
F:CYS287
|
2.2
|
1.1
|
1.0
|
FE2
|
F:SF4601
|
3.1
|
1.1
|
1.0
|
CG
|
F:PRO444
|
3.1
|
9.2
|
1.0
|
FE1
|
F:SF4601
|
3.2
|
18.3
|
1.0
|
CG
|
F:PRO285
|
3.3
|
38.7
|
1.0
|
FE3
|
F:SF4601
|
3.3
|
8.8
|
1.0
|
CB
|
F:PRO285
|
3.7
|
35.6
|
1.0
|
CB
|
F:CYS287
|
3.8
|
27.9
|
1.0
|
CD
|
F:PRO444
|
3.9
|
16.7
|
1.0
|
CB
|
F:PRO444
|
4.0
|
18.1
|
1.0
|
S4
|
F:SF4601
|
4.0
|
1.1
|
1.0
|
SG
|
F:CYS367
|
4.1
|
9.6
|
1.0
|
CA
|
F:PRO444
|
4.1
|
32.7
|
1.0
|
N
|
F:PRO444
|
4.4
|
20.9
|
1.0
|
N
|
F:CYS287
|
4.7
|
35.0
|
1.0
|
CD
|
F:PRO285
|
4.7
|
37.6
|
1.0
|
CE
|
F:MET288
|
4.8
|
21.2
|
1.0
|
SG
|
F:CYS424
|
4.9
|
43.8
|
1.0
|
CA
|
F:CYS287
|
4.9
|
32.3
|
1.0
|
|
Reference:
A.G.Baranovskiy,
N.D.Babayeva,
Y.Zhang,
J.Gu,
Y.Suwa,
Y.I.Pavlov,
T.H.Tahirov.
Mechanism of Concerted Rna-Dna Primer Synthesis By the Human Primosome. J.Biol.Chem. V. 291 10006 2016.
ISSN: ESSN 1083-351X
PubMed: 26975377
DOI: 10.1074/JBC.M116.717405
Page generated: Tue Aug 6 00:37:03 2024
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