Iron in PDB 5n07: Structure of the [4FE-4S] Form of the No Response Regulator Nsrr

The structure of Structure of the [4FE-4S] Form of the No Response Regulator Nsrr, PDB code: 5n07 was solved by A.Volbeda, J.C.Fontecilla-Camps, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.95
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	78.760, 78.760, 93.260, 90.00, 90.00, 120.00
R / Rfree (%)	19.5 / 22.7

The structure of Structure of the [4FE-4S] Form of the No Response Regulator Nsrr also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Iron atom in the Structure of the [4FE-4S] Form of the No Response Regulator Nsrr (pdb code 5n07). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Structure of the [4FE-4S] Form of the No Response Regulator Nsrr, PDB code: 5n07:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Structure of the [4FE-4S] Form of the No Response Regulator Nsrr


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Structure of the [4FE-4S] Form of the No Response Regulator Nsrr within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:70.4 occ:1.00 FE1 A:SF4201 0.0 70.4 1.0 SG A:CYS93 2.3 79.1 1.0 S2 A:SF4201 2.3 75.2 1.0 S3 A:SF4201 2.3 67.7 1.0 S4 A:SF4201 2.3 72.6 1.0 FE3 A:SF4201 2.6 72.1 1.0 FE4 A:SF4201 2.6 73.8 1.0 FE2 A:SF4201 2.7 68.0 1.0 CB A:CYS93 3.1 80.5 1.0 CA A:CYS93 3.8 82.9 1.0 S1 A:SF4201 3.8 72.6 1.0 CG1 A:VAL91 4.3 81.3 1.0 CD A:ARG108 4.4 81.1 1.0 N A:CYS93 4.5 82.0 1.0 CG2 A:VAL91 4.5 86.7 1.0 CB A:ARG108 4.6 80.8 1.0 SG A:CYS105 4.6 69.9 1.0 CB A:CYS99 4.7 83.5 1.0 SG A:CYS99 4.7 77.7 1.0 CG A:ARG108 4.9 82.4 1.0 O A:HOH309 4.9 79.0 1.0

Iron binding site 2 out of 4 in the Structure of the [4FE-4S] Form of the No Response Regulator Nsrr


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Structure of the [4FE-4S] Form of the No Response Regulator Nsrr within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:68.0 occ:1.00 FE2 A:SF4201 0.0 68.0 1.0 S1 A:SF4201 2.2 72.6 1.0 S4 A:SF4201 2.3 72.6 1.0 S3 A:SF4201 2.3 67.7 1.0 FE3 A:SF4201 2.6 72.1 1.0 FE4 A:SF4201 2.7 73.8 1.0 FE1 A:SF4201 2.7 70.4 1.0 S2 A:SF4201 3.9 75.2 1.0 CG2 A:VAL91 4.1 86.7 1.0 CD2 A:LEU107 4.2 68.5 1.0 SG A:CYS99 4.7 77.7 1.0 SG A:CYS93 4.8 79.1 1.0 SG A:CYS105 4.8 69.9 1.0 CG A:LEU107 4.9 68.5 1.0

Iron binding site 3 out of 4 in the Structure of the [4FE-4S] Form of the No Response Regulator Nsrr


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Structure of the [4FE-4S] Form of the No Response Regulator Nsrr within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:72.1 occ:1.00 FE3 A:SF4201 0.0 72.1 1.0 S1 A:SF4201 2.3 72.6 1.0 S4 A:SF4201 2.3 72.6 1.0 SG A:CYS99 2.3 77.7 1.0 S2 A:SF4201 2.3 75.2 1.0 FE1 A:SF4201 2.6 70.4 1.0 FE4 A:SF4201 2.6 73.8 1.0 FE2 A:SF4201 2.6 68.0 1.0 CB A:CYS99 3.0 83.5 1.0 S3 A:SF4201 3.8 67.7 1.0 CB A:LEU101 4.2 79.0 1.0 CA A:CYS99 4.5 87.3 1.0 SG A:CYS105 4.5 69.9 1.0 SG A:CYS93 4.6 79.1 1.0 CB A:CYS93 4.7 80.5 1.0 CD1 A:LEU101 4.8 76.2 1.0 N A:ARG102 4.8 80.3 1.0 CG A:LEU101 4.8 82.7 1.0 N A:LEU101 4.9 86.4 1.0 CA A:LEU101 4.9 85.9 1.0 C A:CYS99 5.0 88.3 1.0 CA A:CYS93 5.0 82.9 1.0 C A:LEU101 5.0 83.5 1.0

Iron binding site 4 out of 4 in the Structure of the [4FE-4S] Form of the No Response Regulator Nsrr


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Structure of the [4FE-4S] Form of the No Response Regulator Nsrr within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:73.8 occ:1.00 FE4 A:SF4201 0.0 73.8 1.0 SG A:CYS105 2.2 69.9 1.0 S3 A:SF4201 2.3 67.7 1.0 S2 A:SF4201 2.3 75.2 1.0 S1 A:SF4201 2.3 72.6 1.0 FE1 A:SF4201 2.6 70.4 1.0 FE3 A:SF4201 2.6 72.1 1.0 FE2 A:SF4201 2.7 68.0 1.0 CB A:CYS105 3.4 74.3 1.0 S4 A:SF4201 3.9 72.6 1.0 N A:ARG108 4.1 70.0 1.0 CB A:ARG108 4.2 80.8 1.0 CB A:LEU107 4.4 69.6 1.0 CD2 A:LEU107 4.5 68.5 1.0 CB A:LEU101 4.6 79.0 1.0 CA A:ARG108 4.6 73.5 1.0 SG A:CYS93 4.6 79.1 1.0 SG A:CYS99 4.7 77.7 1.0 CG A:LEU107 4.7 68.5 1.0 CA A:CYS105 4.7 75.3 1.0 CD1 A:LEU101 4.9 76.2 1.0 C A:LEU107 4.9 69.6 1.0

A.Volbeda, E.L.Dodd, C.Darnault, J.C.Crack, O.Renoux, M.I.Hutchings, N.E.Le Brun, J.C.Fontecilla-Camps. Crystal Structures of the No Sensor Nsrr Reveal How Its Iron-Sulfur Cluster Modulates Dna Binding. Nat Commun V. 8 15052 2017.
ISSN: ESSN 2041-1723
PubMed: 28425466
DOI: 10.1038/NCOMMS15052