Iron in PDB 5v33: R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip

The structure of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip, PDB code: 5v33 was solved by J.M.Schieferstein, A.S.Pawate, C.Sun, F.Wan, J.Broecker, O.P.Ernst, R.B.Gennis, P.J.A.Kenis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.08 / 3.49
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	102.332, 102.332, 240.333, 90.00, 90.00, 90.00
R / Rfree (%)	22.1 / 26.2

The structure of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip also contains other interesting chemical elements:

Magnesium

(Mg)

4 atoms

The binding sites of Iron atom in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip (pdb code 5v33). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip, PDB code: 5v33:

Iron binding site 1 out of 1 in the R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of R. Sphaeroides Photosythetic Reaction Center Mutant - Residue L223, Ser to Trp - Room Temperature Structure Solved on X-Ray Transparent Microfluidic Chip within 5.0Å range: probe atom residue distance (Å) B Occ L:Fe305 b:24.5 occ:1.00 OE1 M:GLU234 1.9 24.8 1.0 NE2 L:HIS190 2.1 26.6 1.0 NE2 M:HIS219 2.3 24.2 1.0 NE2 L:HIS230 2.4 24.8 1.0 OE2 M:GLU234 2.4 25.1 1.0 CD M:GLU234 2.5 25.0 1.0 NE2 M:HIS266 2.5 25.1 1.0 CE1 L:HIS190 3.0 26.8 1.0 CE1 M:HIS219 3.1 24.2 1.0 CD2 L:HIS190 3.2 26.7 1.0 CE1 L:HIS230 3.3 28.1 1.0 CD2 L:HIS230 3.4 24.8 1.0 CD2 M:HIS219 3.4 24.3 1.0 CE1 M:HIS266 3.4 25.3 1.0 CD2 M:HIS266 3.4 24.5 1.0 CG M:GLU234 3.9 25.4 1.0 ND1 L:HIS190 4.1 27.2 1.0 CG L:HIS190 4.3 27.1 1.0 ND1 M:HIS219 4.3 24.1 1.0 ND1 L:HIS230 4.4 25.4 1.0 CG M:HIS219 4.5 24.1 1.0 ND1 M:HIS266 4.5 24.7 1.0 CG L:HIS230 4.5 25.2 1.0 CG M:HIS266 4.5 24.6 1.0 CG1 M:ILE223 4.6 24.9 1.0 CG2 L:VAL194 4.7 25.3 1.0 CD1 M:ILE223 4.9 25.0 1.0 CB M:GLU234 4.9 25.6 1.0

J.M.Schieferstein, A.S.Pawate, C.Sun, F.Wan, P.N.Sheraden, J.Broecker, O.P.Ernst, R.B.Gennis, P.J.A.Kenis. X-Ray Transparent Microfluidic Chips For High-Throughput Screening and Optimization of in Meso Membrane Protein Crystallization. Biomicrofluidics V. 11 24118 2017.
ISSN: ESSN 1932-1058
PubMed: 28469762
DOI: 10.1063/1.4981818