Iron in PDB 5vpn: E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Enzymatic activity of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
All present enzymatic activity of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation:
1.3.5.1;
1.3.5.4;
Protein crystallography data
The structure of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation, PDB code: 5vpn
was solved by
C.A.Starbird,
E.Maklashina,
P.Sharma,
S.Qualls-Histed,
G.Cecchini,
T.M.Iverson,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
22.96 /
4.22
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
133.565,
138.134,
220.079,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
21.5 /
27
|
Iron Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
18;
Binding sites:
The binding sites of Iron atom in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
(pdb code 5vpn). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 18 binding sites of Iron where determined in the
E. Coli Quinol Fumarate Reductase Frda E245Q Mutation, PDB code: 5vpn:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Iron binding site 1 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 1 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe301
b:1.0
occ:1.00
|
FE1
|
B:FES301
|
0.0
|
1.0
|
1.0
|
S1
|
B:FES301
|
2.2
|
0.8
|
1.0
|
S2
|
B:FES301
|
2.2
|
0.2
|
1.0
|
SG
|
B:CYS77
|
2.9
|
0.7
|
1.0
|
CB
|
B:CYS65
|
3.0
|
0.1
|
1.0
|
FE2
|
B:FES301
|
3.1
|
0.7
|
1.0
|
SG
|
B:CYS65
|
3.1
|
0.3
|
1.0
|
CB
|
B:CYS77
|
3.5
|
0.8
|
1.0
|
N
|
B:ALA60
|
4.0
|
0.6
|
1.0
|
CA
|
B:ALA60
|
4.0
|
0.1
|
1.0
|
CA
|
B:CYS65
|
4.3
|
0.3
|
1.0
|
N
|
B:CYS77
|
4.4
|
0.6
|
1.0
|
N
|
B:CYS65
|
4.4
|
0.1
|
1.0
|
CB
|
B:LEU75
|
4.5
|
0.9
|
1.0
|
SG
|
B:CYS57
|
4.5
|
0.1
|
1.0
|
CA
|
B:CYS77
|
4.5
|
0.5
|
1.0
|
CD1
|
B:LEU75
|
4.6
|
98.3
|
1.0
|
CB
|
B:ALA60
|
4.7
|
0.5
|
1.0
|
CG
|
B:LEU75
|
4.9
|
0.8
|
1.0
|
CD2
|
B:LEU75
|
4.9
|
0.3
|
1.0
|
N
|
B:MET59
|
5.0
|
0.5
|
1.0
|
|
Iron binding site 2 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 2 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe301
b:0.7
occ:1.00
|
FE2
|
B:FES301
|
0.0
|
0.7
|
1.0
|
S1
|
B:FES301
|
2.2
|
0.8
|
1.0
|
S2
|
B:FES301
|
2.2
|
0.2
|
1.0
|
SG
|
B:CYS57
|
2.8
|
0.1
|
1.0
|
SG
|
B:CYS62
|
2.9
|
0.5
|
1.0
|
FE1
|
B:FES301
|
3.1
|
1.0
|
1.0
|
CB
|
B:CYS62
|
3.2
|
0.8
|
1.0
|
N
|
B:CYS57
|
3.2
|
0.3
|
1.0
|
CB
|
B:CYS57
|
3.5
|
0.4
|
1.0
|
N
|
B:GLY63
|
3.8
|
0.0
|
1.0
|
CA
|
B:CYS57
|
3.9
|
0.8
|
1.0
|
N
|
B:CYS62
|
3.9
|
0.3
|
1.0
|
N
|
B:SER56
|
3.9
|
0.2
|
1.0
|
CA
|
B:CYS62
|
4.0
|
0.2
|
1.0
|
N
|
B:ARG58
|
4.0
|
0.8
|
1.0
|
C
|
B:SER56
|
4.2
|
0.5
|
1.0
|
OG
|
B:SER64
|
4.2
|
0.9
|
1.0
|
CA
|
B:SER56
|
4.4
|
0.4
|
1.0
|
C
|
B:CYS57
|
4.4
|
0.8
|
1.0
|
C
|
B:CYS62
|
4.4
|
0.6
|
1.0
|
N
|
B:SER64
|
4.4
|
0.2
|
1.0
|
CB
|
B:SER56
|
4.5
|
0.1
|
1.0
|
N
|
B:ILE61
|
4.7
|
0.6
|
1.0
|
N
|
B:ALA60
|
4.7
|
0.6
|
1.0
|
CA
|
B:GLY63
|
4.8
|
0.8
|
1.0
|
C
|
B:TRP55
|
4.8
|
0.5
|
1.0
|
N
|
B:CYS65
|
5.0
|
0.1
|
1.0
|
CB
|
B:CYS65
|
5.0
|
0.1
|
1.0
|
|
Iron binding site 3 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 3 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe302
b:0.0
occ:1.00
|
FE1
|
B:F3S302
|
0.0
|
0.0
|
1.0
|
S1
|
B:F3S302
|
2.2
|
0.0
|
1.0
|
S2
|
B:F3S302
|
2.3
|
0.7
|
1.0
|
S3
|
B:F3S302
|
2.3
|
0.7
|
1.0
|
FE3
|
B:F3S302
|
2.7
|
0.3
|
1.0
|
FE4
|
B:F3S302
|
2.7
|
0.5
|
1.0
|
SG
|
B:CYS204
|
2.9
|
0.6
|
1.0
|
CB
|
B:CYS204
|
3.0
|
0.8
|
1.0
|
CD1
|
B:ILE224
|
3.5
|
0.0
|
1.0
|
CA
|
B:CYS204
|
3.8
|
1.0
|
1.0
|
S4
|
B:F3S302
|
4.0
|
0.9
|
1.0
|
N
|
B:PHE206
|
4.3
|
0.7
|
1.0
|
CA
|
B:PHE206
|
4.5
|
0.4
|
1.0
|
N
|
B:VAL207
|
4.5
|
0.5
|
1.0
|
C
|
B:CYS204
|
4.6
|
0.3
|
1.0
|
N
|
B:THR205
|
4.6
|
0.6
|
1.0
|
CG2
|
B:ILE224
|
4.8
|
0.7
|
1.0
|
CG1
|
B:ILE224
|
4.9
|
0.2
|
1.0
|
SG
|
B:CYS158
|
5.0
|
0.3
|
1.0
|
CZ
|
B:PHE167
|
5.0
|
0.1
|
1.0
|
|
Iron binding site 4 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 4 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe302
b:0.3
occ:1.00
|
FE3
|
B:F3S302
|
0.0
|
0.3
|
1.0
|
S1
|
B:F3S302
|
2.2
|
0.0
|
1.0
|
S4
|
B:F3S302
|
2.3
|
0.9
|
1.0
|
S3
|
B:F3S302
|
2.3
|
0.7
|
1.0
|
FE1
|
B:F3S302
|
2.7
|
0.0
|
1.0
|
FE4
|
B:F3S302
|
2.7
|
0.5
|
1.0
|
SG
|
B:CYS210
|
2.9
|
0.8
|
1.0
|
CB
|
B:CYS210
|
3.6
|
0.4
|
1.0
|
S2
|
B:F3S302
|
3.9
|
0.7
|
1.0
|
N
|
B:GLY208
|
4.0
|
0.3
|
1.0
|
N
|
B:CYS210
|
4.2
|
0.8
|
1.0
|
CD1
|
B:ILE224
|
4.2
|
0.0
|
1.0
|
CA
|
B:GLY208
|
4.4
|
0.8
|
1.0
|
CB
|
B:ALA221
|
4.4
|
1.0
|
1.0
|
CA
|
B:CYS210
|
4.5
|
0.5
|
1.0
|
N
|
B:TYR209
|
4.6
|
0.9
|
1.0
|
C
|
B:GLY208
|
4.8
|
0.7
|
1.0
|
N
|
B:VAL207
|
4.8
|
0.5
|
1.0
|
CA
|
B:ALA221
|
4.8
|
0.5
|
1.0
|
CB
|
B:CYS158
|
4.9
|
0.0
|
1.0
|
CB
|
B:PRO170
|
4.9
|
0.5
|
1.0
|
|
Iron binding site 5 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 5 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe302
b:0.5
occ:1.00
|
FE4
|
B:F3S302
|
0.0
|
0.5
|
1.0
|
S2
|
B:F3S302
|
2.3
|
0.7
|
1.0
|
S4
|
B:F3S302
|
2.3
|
0.9
|
1.0
|
S3
|
B:F3S302
|
2.3
|
0.7
|
1.0
|
FE3
|
B:F3S302
|
2.7
|
0.3
|
1.0
|
FE1
|
B:F3S302
|
2.7
|
0.0
|
1.0
|
SG
|
B:CYS158
|
3.0
|
0.3
|
1.0
|
CB
|
B:CYS158
|
3.1
|
0.0
|
1.0
|
CA
|
B:CYS158
|
3.6
|
0.9
|
1.0
|
S1
|
B:F3S302
|
3.9
|
0.0
|
1.0
|
CD
|
B:PRO159
|
4.0
|
0.8
|
1.0
|
CB
|
B:VAL207
|
4.3
|
0.6
|
1.0
|
C
|
B:CYS158
|
4.5
|
0.1
|
1.0
|
N
|
B:PRO159
|
4.5
|
0.2
|
1.0
|
CG2
|
B:VAL207
|
4.6
|
1.0
|
1.0
|
N
|
B:VAL207
|
4.6
|
0.5
|
1.0
|
N
|
B:CYS158
|
4.7
|
0.7
|
1.0
|
N
|
B:GLY208
|
4.9
|
0.3
|
1.0
|
CB
|
B:GLN160
|
4.9
|
0.5
|
1.0
|
N
|
B:GLN160
|
5.0
|
0.8
|
1.0
|
CA
|
B:VAL207
|
5.0
|
0.9
|
1.0
|
|
Iron binding site 6 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 6 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe303
b:0.8
occ:1.00
|
FE1
|
B:SF4303
|
0.0
|
0.8
|
1.0
|
S4
|
B:SF4303
|
2.2
|
0.4
|
1.0
|
S3
|
B:SF4303
|
2.2
|
0.4
|
1.0
|
S2
|
B:SF4303
|
2.2
|
0.9
|
1.0
|
SG
|
B:CYS151
|
2.9
|
0.7
|
1.0
|
N
|
B:GLY152
|
3.1
|
0.8
|
1.0
|
FE2
|
B:SF4303
|
3.1
|
0.7
|
1.0
|
FE4
|
B:SF4303
|
3.1
|
0.5
|
1.0
|
FE3
|
B:SF4303
|
3.1
|
0.8
|
1.0
|
N
|
B:CYS151
|
3.5
|
0.5
|
1.0
|
N
|
B:LEU153
|
3.6
|
0.8
|
1.0
|
CB
|
B:CYS151
|
3.7
|
0.2
|
1.0
|
C
|
B:CYS151
|
3.8
|
0.2
|
1.0
|
S1
|
B:SF4303
|
3.8
|
0.6
|
1.0
|
CA
|
B:CYS151
|
3.8
|
0.2
|
1.0
|
CA
|
B:GLY152
|
3.9
|
0.6
|
1.0
|
C
|
B:GLY152
|
4.2
|
0.7
|
1.0
|
N
|
B:CYS154
|
4.4
|
0.5
|
1.0
|
CB
|
B:LEU153
|
4.5
|
0.2
|
1.0
|
C
|
B:ASN150
|
4.5
|
0.6
|
1.0
|
CA
|
B:LEU153
|
4.5
|
0.7
|
1.0
|
SG
|
B:CYS154
|
4.6
|
0.3
|
1.0
|
N
|
B:ASN150
|
4.7
|
1.0
|
1.0
|
CD
|
B:PRO215
|
4.8
|
0.4
|
1.0
|
CG1
|
B:ILE149
|
4.9
|
0.8
|
1.0
|
CA
|
B:ASN150
|
4.9
|
1.0
|
1.0
|
O
|
B:CYS151
|
4.9
|
0.6
|
1.0
|
|
Iron binding site 7 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 7 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe303
b:0.7
occ:1.00
|
FE2
|
B:SF4303
|
0.0
|
0.7
|
1.0
|
S4
|
B:SF4303
|
2.2
|
0.4
|
1.0
|
S1
|
B:SF4303
|
2.2
|
0.6
|
1.0
|
S3
|
B:SF4303
|
2.2
|
0.4
|
1.0
|
SG
|
B:CYS154
|
2.8
|
0.3
|
1.0
|
FE4
|
B:SF4303
|
3.0
|
0.5
|
1.0
|
FE3
|
B:SF4303
|
3.1
|
0.8
|
1.0
|
FE1
|
B:SF4303
|
3.1
|
0.8
|
1.0
|
CB
|
B:CYS154
|
3.5
|
0.6
|
1.0
|
S2
|
B:SF4303
|
3.8
|
0.9
|
1.0
|
CB
|
B:ALA171
|
4.0
|
0.2
|
1.0
|
N
|
B:CYS154
|
4.4
|
0.5
|
1.0
|
CA
|
B:ALA171
|
4.5
|
1.0
|
1.0
|
CG
|
B:PRO220
|
4.5
|
0.7
|
1.0
|
CA
|
B:CYS154
|
4.5
|
0.2
|
1.0
|
N
|
B:ALA171
|
4.8
|
0.9
|
1.0
|
CB
|
B:PRO220
|
5.0
|
0.6
|
1.0
|
|
Iron binding site 8 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 8 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe303
b:0.8
occ:1.00
|
FE3
|
B:SF4303
|
0.0
|
0.8
|
1.0
|
S4
|
B:SF4303
|
2.2
|
0.4
|
1.0
|
S2
|
B:SF4303
|
2.2
|
0.9
|
1.0
|
S1
|
B:SF4303
|
2.2
|
0.6
|
1.0
|
SG
|
B:CYS148
|
2.9
|
0.2
|
1.0
|
CB
|
B:CYS148
|
3.1
|
0.9
|
1.0
|
FE2
|
B:SF4303
|
3.1
|
0.7
|
1.0
|
FE4
|
B:SF4303
|
3.1
|
0.5
|
1.0
|
FE1
|
B:SF4303
|
3.1
|
0.8
|
1.0
|
CA
|
B:CYS148
|
3.2
|
0.1
|
1.0
|
N
|
B:ILE149
|
3.4
|
0.6
|
1.0
|
C
|
B:CYS148
|
3.6
|
0.8
|
1.0
|
S3
|
B:SF4303
|
3.8
|
0.4
|
1.0
|
N
|
B:ASN150
|
3.8
|
1.0
|
1.0
|
CG2
|
B:VAL218
|
4.3
|
0.7
|
1.0
|
CA
|
B:ILE149
|
4.5
|
0.7
|
1.0
|
CA
|
B:ASN150
|
4.6
|
1.0
|
1.0
|
N
|
B:CYS148
|
4.6
|
0.8
|
1.0
|
C
|
B:ILE149
|
4.6
|
0.3
|
1.0
|
O
|
B:CYS148
|
4.6
|
0.9
|
1.0
|
N
|
B:CYS151
|
4.7
|
0.5
|
1.0
|
CG1
|
B:ILE149
|
4.8
|
0.8
|
1.0
|
CB
|
B:ALA171
|
4.9
|
0.2
|
1.0
|
|
Iron binding site 9 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 9 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 9 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe303
b:0.5
occ:1.00
|
FE4
|
B:SF4303
|
0.0
|
0.5
|
1.0
|
S3
|
B:SF4303
|
2.2
|
0.4
|
1.0
|
S2
|
B:SF4303
|
2.2
|
0.9
|
1.0
|
S1
|
B:SF4303
|
2.2
|
0.6
|
1.0
|
SG
|
B:CYS214
|
2.9
|
0.7
|
1.0
|
FE2
|
B:SF4303
|
3.0
|
0.7
|
1.0
|
FE3
|
B:SF4303
|
3.1
|
0.8
|
1.0
|
FE1
|
B:SF4303
|
3.1
|
0.8
|
1.0
|
CB
|
B:CYS214
|
3.6
|
0.8
|
1.0
|
CD
|
B:PRO215
|
3.6
|
0.4
|
1.0
|
CA
|
B:CYS214
|
3.7
|
0.8
|
1.0
|
S4
|
B:SF4303
|
3.8
|
0.4
|
1.0
|
N
|
B:PRO215
|
4.2
|
0.2
|
1.0
|
CG2
|
B:VAL218
|
4.3
|
0.7
|
1.0
|
C
|
B:CYS214
|
4.3
|
0.0
|
1.0
|
CG
|
B:PRO215
|
4.6
|
0.8
|
1.0
|
N
|
B:LYS216
|
4.6
|
0.4
|
1.0
|
CB
|
B:VAL218
|
4.7
|
0.7
|
1.0
|
N
|
B:CYS214
|
4.9
|
0.6
|
1.0
|
|
Iron binding site 10 out
of 18 in 5vpn
Go back to
Iron Binding Sites List in 5vpn
Iron binding site 10 out
of 18 in the E. Coli Quinol Fumarate Reductase Frda E245Q Mutation
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 10 of E. Coli Quinol Fumarate Reductase Frda E245Q Mutation within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Fe301
b:0.6
occ:1.00
|
FE1
|
F:FES301
|
0.0
|
0.6
|
1.0
|
S2
|
F:FES301
|
2.2
|
0.6
|
1.0
|
S1
|
F:FES301
|
2.2
|
0.6
|
1.0
|
SG
|
F:CYS77
|
2.9
|
0.2
|
1.0
|
CB
|
F:CYS65
|
3.0
|
0.1
|
1.0
|
SG
|
F:CYS65
|
3.1
|
0.2
|
1.0
|
FE2
|
F:FES301
|
3.1
|
0.4
|
1.0
|
CB
|
F:CYS77
|
3.3
|
0.9
|
1.0
|
N
|
F:ALA60
|
3.8
|
0.8
|
1.0
|
CA
|
F:ALA60
|
3.9
|
0.2
|
1.0
|
CA
|
F:CYS65
|
4.3
|
0.9
|
1.0
|
N
|
F:CYS77
|
4.4
|
0.3
|
1.0
|
CB
|
F:LEU75
|
4.4
|
0.1
|
1.0
|
CA
|
F:CYS77
|
4.5
|
0.2
|
1.0
|
N
|
F:CYS65
|
4.5
|
0.2
|
1.0
|
SG
|
F:CYS57
|
4.6
|
0.9
|
1.0
|
CB
|
F:ALA60
|
4.6
|
0.4
|
1.0
|
N
|
F:MET59
|
4.7
|
0.5
|
1.0
|
CD1
|
F:LEU75
|
4.7
|
0.9
|
1.0
|
|
Reference:
C.A.Starbird,
E.Maklashina,
P.Sharma,
S.Qualls-Histed,
G.Cecchini,
T.M.Iverson.
Structural and Biochemical Analyses Reveal Insights Into Covalent Flavinylation of the Escherichia Coli Complex II Homolog Quinol:Fumarate Reductase. J. Biol. Chem. V. 292 12921 2017.
ISSN: ESSN 1083-351X
PubMed: 28615448
DOI: 10.1074/JBC.M117.795120
Page generated: Tue Aug 6 10:29:34 2024
|