Iron in PDB 5zzn: Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Enzymatic activity of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
All present enzymatic activity of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus:
1.10.3.9;
Protein crystallography data
The structure of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus, PDB code: 5zzn
was solved by
Y.Nakajima,
Y.Umena,
R.Nagao,
K.Endo,
K.Kobayashi,
F.Akita,
M.Suga,
H.Wada,
T.Noguchi,
J.R.Shen,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
19.99 /
2.10
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
122.250,
228.430,
287.120,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
15.4 /
19.7
|
Other elements in 5zzn:
The structure of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
(pdb code 5zzn). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 6 binding sites of Iron where determined in the
Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus, PDB code: 5zzn:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
Iron binding site 1 out
of 6 in 5zzn
Go back to
Iron Binding Sites List in 5zzn
Iron binding site 1 out
of 6 in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Fe401
b:41.5
occ:1.00
|
NE2
|
A:HIS215
|
2.3
|
41.2
|
1.0
|
O1
|
D:BCT402
|
2.3
|
24.5
|
0.4
|
NE2
|
D:HIS214
|
2.3
|
36.3
|
1.0
|
NE2
|
D:HIS268
|
2.3
|
34.8
|
1.0
|
O1
|
D:BCT402
|
2.4
|
50.8
|
0.6
|
NE2
|
A:HIS272
|
2.4
|
44.7
|
1.0
|
O2
|
D:BCT402
|
2.5
|
36.0
|
0.6
|
O2
|
D:BCT402
|
2.6
|
36.5
|
0.4
|
C
|
D:BCT402
|
2.7
|
46.6
|
0.6
|
C
|
D:BCT402
|
2.7
|
36.3
|
0.4
|
CE1
|
A:HIS215
|
3.1
|
37.8
|
1.0
|
CE1
|
D:HIS268
|
3.2
|
37.2
|
1.0
|
CE1
|
A:HIS272
|
3.2
|
35.6
|
1.0
|
CD2
|
D:HIS214
|
3.2
|
34.8
|
1.0
|
CE1
|
D:HIS214
|
3.3
|
35.7
|
1.0
|
CD2
|
A:HIS215
|
3.4
|
38.5
|
1.0
|
CD2
|
D:HIS268
|
3.4
|
36.1
|
1.0
|
CD2
|
A:HIS272
|
3.5
|
45.0
|
1.0
|
O3
|
D:BCT402
|
4.0
|
44.2
|
0.6
|
O3
|
D:BCT402
|
4.0
|
40.4
|
0.4
|
ND1
|
A:HIS215
|
4.3
|
38.9
|
1.0
|
ND1
|
D:HIS268
|
4.4
|
35.0
|
1.0
|
CG
|
D:HIS214
|
4.4
|
33.8
|
1.0
|
ND1
|
A:HIS272
|
4.4
|
39.9
|
1.0
|
ND1
|
D:HIS214
|
4.4
|
38.5
|
1.0
|
CG
|
A:HIS215
|
4.4
|
38.6
|
1.0
|
CG
|
D:HIS268
|
4.5
|
34.8
|
1.0
|
CG
|
A:HIS272
|
4.6
|
37.8
|
1.0
|
CG2
|
A:VAL219
|
4.7
|
33.6
|
1.0
|
CG2
|
D:VAL218
|
4.9
|
30.1
|
1.0
|
|
Iron binding site 2 out
of 6 in 5zzn
Go back to
Iron Binding Sites List in 5zzn
Iron binding site 2 out
of 6 in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
F:Fe101
b:49.0
occ:1.00
|
FE
|
F:HEM101
|
0.0
|
49.0
|
1.0
|
NB
|
F:HEM101
|
1.9
|
48.7
|
1.0
|
ND
|
F:HEM101
|
2.1
|
49.0
|
1.0
|
NA
|
F:HEM101
|
2.1
|
56.1
|
1.0
|
NC
|
F:HEM101
|
2.1
|
48.8
|
1.0
|
NE2
|
E:HIS23
|
2.2
|
56.0
|
1.0
|
NE2
|
F:HIS24
|
2.2
|
51.0
|
1.0
|
C4B
|
F:HEM101
|
3.0
|
55.7
|
1.0
|
C1B
|
F:HEM101
|
3.0
|
56.8
|
1.0
|
C4D
|
F:HEM101
|
3.0
|
57.8
|
1.0
|
CD2
|
F:HIS24
|
3.0
|
53.6
|
1.0
|
C1A
|
F:HEM101
|
3.1
|
53.5
|
1.0
|
CE1
|
E:HIS23
|
3.1
|
58.4
|
1.0
|
C1D
|
F:HEM101
|
3.1
|
52.8
|
1.0
|
C1C
|
F:HEM101
|
3.1
|
43.3
|
1.0
|
C4A
|
F:HEM101
|
3.1
|
56.5
|
1.0
|
C4C
|
F:HEM101
|
3.1
|
46.8
|
1.0
|
CD2
|
E:HIS23
|
3.2
|
58.5
|
1.0
|
CE1
|
F:HIS24
|
3.2
|
52.3
|
1.0
|
CHA
|
F:HEM101
|
3.4
|
53.3
|
1.0
|
CHC
|
F:HEM101
|
3.4
|
51.9
|
1.0
|
CHB
|
F:HEM101
|
3.4
|
56.4
|
1.0
|
CHD
|
F:HEM101
|
3.5
|
47.2
|
1.0
|
C3B
|
F:HEM101
|
4.2
|
52.9
|
1.0
|
C2B
|
F:HEM101
|
4.2
|
59.5
|
1.0
|
ND1
|
E:HIS23
|
4.2
|
55.7
|
1.0
|
CG
|
F:HIS24
|
4.2
|
57.5
|
1.0
|
C3D
|
F:HEM101
|
4.3
|
50.4
|
1.0
|
ND1
|
F:HIS24
|
4.3
|
53.8
|
1.0
|
C2D
|
F:HEM101
|
4.3
|
52.5
|
1.0
|
CG
|
E:HIS23
|
4.3
|
61.6
|
1.0
|
C2A
|
F:HEM101
|
4.3
|
54.0
|
1.0
|
C2C
|
F:HEM101
|
4.3
|
49.0
|
1.0
|
C3A
|
F:HEM101
|
4.3
|
63.3
|
1.0
|
C3C
|
F:HEM101
|
4.3
|
52.1
|
1.0
|
CE1
|
E:TYR19
|
4.9
|
64.2
|
1.0
|
NE1
|
F:TRP20
|
4.9
|
49.3
|
1.0
|
|
Iron binding site 3 out
of 6 in 5zzn
Go back to
Iron Binding Sites List in 5zzn
Iron binding site 3 out
of 6 in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
V:Fe201
b:33.2
occ:1.00
|
FE
|
V:HEC201
|
0.0
|
33.2
|
1.0
|
NA
|
V:HEC201
|
1.9
|
33.5
|
1.0
|
NC
|
V:HEC201
|
2.0
|
28.3
|
1.0
|
NE2
|
V:HIS41
|
2.0
|
28.1
|
1.0
|
NB
|
V:HEC201
|
2.1
|
32.4
|
1.0
|
ND
|
V:HEC201
|
2.1
|
32.9
|
1.0
|
NE2
|
V:HIS92
|
2.1
|
39.8
|
1.0
|
C4A
|
V:HEC201
|
3.0
|
35.1
|
1.0
|
CE1
|
V:HIS41
|
3.0
|
31.3
|
1.0
|
C4C
|
V:HEC201
|
3.0
|
32.8
|
1.0
|
C1C
|
V:HEC201
|
3.0
|
32.6
|
1.0
|
C1A
|
V:HEC201
|
3.0
|
31.3
|
1.0
|
C1D
|
V:HEC201
|
3.0
|
31.5
|
1.0
|
CD2
|
V:HIS41
|
3.0
|
30.1
|
1.0
|
CD2
|
V:HIS92
|
3.1
|
38.8
|
1.0
|
C1B
|
V:HEC201
|
3.1
|
32.2
|
1.0
|
C4D
|
V:HEC201
|
3.1
|
32.8
|
1.0
|
C4B
|
V:HEC201
|
3.1
|
32.3
|
1.0
|
CE1
|
V:HIS92
|
3.1
|
34.5
|
1.0
|
CHB
|
V:HEC201
|
3.4
|
32.8
|
1.0
|
CHD
|
V:HEC201
|
3.4
|
31.6
|
1.0
|
CHC
|
V:HEC201
|
3.4
|
30.4
|
1.0
|
CHA
|
V:HEC201
|
3.4
|
31.1
|
1.0
|
ND1
|
V:HIS41
|
4.1
|
31.5
|
1.0
|
CG
|
V:HIS41
|
4.2
|
30.8
|
1.0
|
C3A
|
V:HEC201
|
4.2
|
35.4
|
1.0
|
C2A
|
V:HEC201
|
4.2
|
34.6
|
1.0
|
ND1
|
V:HIS92
|
4.2
|
38.0
|
1.0
|
CG
|
V:HIS92
|
4.2
|
38.6
|
1.0
|
C2C
|
V:HEC201
|
4.2
|
33.5
|
1.0
|
C3C
|
V:HEC201
|
4.2
|
31.6
|
1.0
|
C2D
|
V:HEC201
|
4.3
|
35.1
|
1.0
|
C3D
|
V:HEC201
|
4.3
|
31.3
|
1.0
|
C2B
|
V:HEC201
|
4.3
|
34.3
|
1.0
|
C3B
|
V:HEC201
|
4.3
|
36.7
|
1.0
|
CE2
|
V:TYR75
|
4.8
|
35.6
|
1.0
|
|
Iron binding site 4 out
of 6 in 5zzn
Go back to
Iron Binding Sites List in 5zzn
Iron binding site 4 out
of 6 in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
a:Fe403
b:40.9
occ:1.00
|
NE2
|
a:HIS215
|
2.2
|
38.9
|
1.0
|
O1
|
d:BCT401
|
2.3
|
31.6
|
0.4
|
NE2
|
d:HIS214
|
2.3
|
33.1
|
1.0
|
O2
|
d:BCT401
|
2.3
|
22.0
|
0.4
|
NE2
|
a:HIS272
|
2.3
|
41.1
|
1.0
|
NE2
|
d:HIS268
|
2.4
|
30.8
|
1.0
|
O1
|
d:BCT401
|
2.6
|
50.8
|
0.6
|
C
|
d:BCT401
|
2.6
|
31.7
|
0.4
|
O2
|
d:BCT401
|
2.7
|
68.0
|
0.6
|
C
|
d:BCT401
|
3.0
|
55.5
|
0.6
|
CE1
|
a:HIS215
|
3.0
|
39.8
|
1.0
|
CE1
|
a:HIS272
|
3.1
|
38.0
|
1.0
|
CD2
|
d:HIS214
|
3.2
|
33.3
|
1.0
|
CE1
|
d:HIS268
|
3.2
|
29.7
|
1.0
|
CD2
|
a:HIS215
|
3.3
|
35.5
|
1.0
|
CE1
|
d:HIS214
|
3.3
|
34.4
|
1.0
|
CD2
|
a:HIS272
|
3.4
|
40.1
|
1.0
|
CD2
|
d:HIS268
|
3.5
|
32.6
|
1.0
|
O3
|
d:BCT401
|
3.9
|
38.5
|
0.4
|
ND1
|
a:HIS215
|
4.2
|
38.7
|
1.0
|
O3
|
d:BCT401
|
4.3
|
52.1
|
0.6
|
ND1
|
a:HIS272
|
4.3
|
41.6
|
1.0
|
CG
|
a:HIS215
|
4.4
|
36.7
|
1.0
|
CG
|
d:HIS214
|
4.4
|
36.8
|
1.0
|
ND1
|
d:HIS268
|
4.4
|
32.4
|
1.0
|
ND1
|
d:HIS214
|
4.4
|
38.4
|
1.0
|
CG
|
a:HIS272
|
4.5
|
43.2
|
1.0
|
CG
|
d:HIS268
|
4.6
|
28.2
|
1.0
|
CG2
|
a:VAL219
|
4.7
|
34.9
|
1.0
|
CG2
|
d:VAL218
|
4.9
|
30.9
|
1.0
|
|
Iron binding site 5 out
of 6 in 5zzn
Go back to
Iron Binding Sites List in 5zzn
Iron binding site 5 out
of 6 in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
f:Fe101
b:48.6
occ:1.00
|
FE
|
f:HEM101
|
0.0
|
48.6
|
1.0
|
NA
|
f:HEM101
|
2.0
|
48.5
|
1.0
|
ND
|
f:HEM101
|
2.0
|
48.1
|
1.0
|
NE2
|
e:HIS23
|
2.0
|
55.5
|
1.0
|
NC
|
f:HEM101
|
2.1
|
44.1
|
1.0
|
NB
|
f:HEM101
|
2.1
|
42.0
|
1.0
|
NE2
|
f:HIS24
|
2.2
|
54.5
|
1.0
|
CE1
|
e:HIS23
|
2.9
|
51.6
|
1.0
|
C1A
|
f:HEM101
|
3.0
|
49.3
|
1.0
|
C4D
|
f:HEM101
|
3.0
|
50.7
|
1.0
|
C1D
|
f:HEM101
|
3.0
|
50.7
|
1.0
|
C4A
|
f:HEM101
|
3.0
|
52.1
|
1.0
|
C4C
|
f:HEM101
|
3.1
|
46.9
|
1.0
|
CD2
|
f:HIS24
|
3.1
|
45.4
|
1.0
|
C1B
|
f:HEM101
|
3.1
|
53.2
|
1.0
|
CD2
|
e:HIS23
|
3.1
|
57.0
|
1.0
|
C1C
|
f:HEM101
|
3.1
|
46.1
|
1.0
|
C4B
|
f:HEM101
|
3.1
|
46.1
|
1.0
|
CE1
|
f:HIS24
|
3.2
|
48.3
|
1.0
|
CHA
|
f:HEM101
|
3.3
|
53.3
|
1.0
|
CHD
|
f:HEM101
|
3.4
|
48.5
|
1.0
|
CHB
|
f:HEM101
|
3.4
|
54.6
|
1.0
|
CHC
|
f:HEM101
|
3.5
|
47.1
|
1.0
|
ND1
|
e:HIS23
|
4.1
|
47.3
|
1.0
|
CG
|
e:HIS23
|
4.2
|
54.2
|
1.0
|
C2A
|
f:HEM101
|
4.2
|
51.3
|
1.0
|
C2D
|
f:HEM101
|
4.2
|
49.5
|
1.0
|
C3D
|
f:HEM101
|
4.2
|
48.2
|
1.0
|
C3A
|
f:HEM101
|
4.3
|
52.4
|
1.0
|
CG
|
f:HIS24
|
4.3
|
53.0
|
1.0
|
ND1
|
f:HIS24
|
4.3
|
52.5
|
1.0
|
C3C
|
f:HEM101
|
4.3
|
44.5
|
1.0
|
C2C
|
f:HEM101
|
4.3
|
48.6
|
1.0
|
C2B
|
f:HEM101
|
4.4
|
52.6
|
1.0
|
C3B
|
f:HEM101
|
4.4
|
49.2
|
1.0
|
CE1
|
e:TYR19
|
4.7
|
55.6
|
1.0
|
NE1
|
f:TRP20
|
5.0
|
50.2
|
1.0
|
|
Iron binding site 6 out
of 6 in 5zzn
Go back to
Iron Binding Sites List in 5zzn
Iron binding site 6 out
of 6 in the Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of Crystal Structure of Photosystem II From An Sqdg-Deficient Mutant of Thermosynechococcus Elongatus within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
v:Fe202
b:41.7
occ:1.00
|
FE
|
v:HEC202
|
0.0
|
41.7
|
1.0
|
NC
|
v:HEC202
|
2.0
|
48.2
|
1.0
|
NB
|
v:HEC202
|
2.0
|
40.1
|
1.0
|
NE2
|
v:HIS41
|
2.1
|
50.1
|
1.0
|
NA
|
v:HEC202
|
2.1
|
43.4
|
1.0
|
ND
|
v:HEC202
|
2.1
|
45.1
|
1.0
|
NE2
|
v:HIS92
|
2.1
|
39.3
|
1.0
|
C1C
|
v:HEC202
|
3.0
|
44.7
|
1.0
|
C4B
|
v:HEC202
|
3.0
|
44.0
|
1.0
|
CE1
|
v:HIS41
|
3.0
|
45.1
|
1.0
|
C4D
|
v:HEC202
|
3.1
|
42.7
|
1.0
|
CD2
|
v:HIS41
|
3.1
|
43.4
|
1.0
|
C1A
|
v:HEC202
|
3.1
|
43.0
|
1.0
|
CD2
|
v:HIS92
|
3.1
|
41.1
|
1.0
|
C4C
|
v:HEC202
|
3.1
|
53.2
|
1.0
|
C1B
|
v:HEC202
|
3.1
|
45.3
|
1.0
|
CE1
|
v:HIS92
|
3.1
|
41.5
|
1.0
|
C1D
|
v:HEC202
|
3.2
|
47.4
|
1.0
|
C4A
|
v:HEC202
|
3.2
|
44.2
|
1.0
|
CHC
|
v:HEC202
|
3.3
|
47.0
|
1.0
|
CHA
|
v:HEC202
|
3.4
|
42.6
|
1.0
|
CHB
|
v:HEC202
|
3.5
|
44.0
|
1.0
|
CHD
|
v:HEC202
|
3.5
|
44.3
|
1.0
|
ND1
|
v:HIS41
|
4.1
|
42.2
|
1.0
|
CG
|
v:HIS41
|
4.2
|
44.1
|
1.0
|
ND1
|
v:HIS92
|
4.2
|
43.9
|
1.0
|
CG
|
v:HIS92
|
4.2
|
39.3
|
1.0
|
C2C
|
v:HEC202
|
4.2
|
43.7
|
1.0
|
C3B
|
v:HEC202
|
4.3
|
44.4
|
1.0
|
C3D
|
v:HEC202
|
4.3
|
41.7
|
1.0
|
C2B
|
v:HEC202
|
4.3
|
46.6
|
1.0
|
C3C
|
v:HEC202
|
4.3
|
44.3
|
1.0
|
C2A
|
v:HEC202
|
4.3
|
47.5
|
1.0
|
C2D
|
v:HEC202
|
4.3
|
40.6
|
1.0
|
C3A
|
v:HEC202
|
4.4
|
45.6
|
1.0
|
CE2
|
v:TYR75
|
4.9
|
48.2
|
1.0
|
|
Reference:
Y.Nakajima,
Y.Umena,
R.Nagao,
K.Endo,
K.Kobayashi,
F.Akita,
M.Suga,
H.Wada,
T.Noguchi,
J.R.Shen.
Thylakoid Membrane Lipid Sulfoquinovosyl-Diacylglycerol (Sqdg) Is Required For Full Functioning of Photosystem II Inthermosynechococcus Elongatus. J. Biol. Chem. V. 293 14786 2018.
ISSN: ESSN 1083-351X
PubMed: 30076221
DOI: 10.1074/JBC.RA118.004304
Page generated: Tue Aug 6 13:23:58 2024
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