Iron in PDB 6aln: Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG

All present enzymatic activity of Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG:
1.14.11.41;

The structure of Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG, PDB code: 6aln was solved by A.J.Mitchell, N.P.Dunham, A.K.Boal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.80
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	80.760, 66.653, 62.889, 90.00, 109.21, 90.00
R / Rfree (%)	17.9 / 21.2

The binding sites of Iron atom in the Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG (pdb code 6aln). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG, PDB code: 6aln:

Iron binding site 1 out of 1 in the Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and 2OG within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe402 b:16.9 occ:1.00 O1 A:AKG401 2.0 20.7 1.0 OE1 A:GLU170 2.1 16.9 1.0 NE2 A:HIS316 2.2 14.6 1.0 O5 A:AKG401 2.3 18.9 1.0 NE2 A:HIS168 2.3 15.4 1.0 OB A:ZZU403 2.6 26.4 1.0 C1 A:AKG401 2.8 20.8 1.0 C2 A:AKG401 2.9 19.8 1.0 CE1 A:HIS316 3.0 14.5 1.0 CD A:GLU170 3.1 17.1 1.0 CE1 A:HIS168 3.1 15.2 1.0 CD2 A:HIS316 3.2 14.5 1.0 CD2 A:HIS168 3.3 15.4 1.0 OE2 A:GLU170 3.4 17.7 1.0 CB A:ZZU403 3.6 25.8 1.0 O2 A:AKG401 4.0 21.6 1.0 ND1 A:HIS316 4.1 14.3 1.0 CG A:ZZU403 4.2 26.3 1.0 CG A:HIS316 4.2 14.2 1.0 ND1 A:HIS168 4.2 15.0 1.0 C3 A:AKG401 4.4 19.4 1.0 CG A:HIS168 4.4 14.9 1.0 CG A:GLU170 4.4 16.4 1.0 CB A:GLU170 4.7 15.8 1.0 NH1 A:ARG334 4.8 23.0 1.0 CD2 A:LEU165 4.9 17.3 1.0 CA A:ZZU403 4.9 25.1 1.0 O A:HIS316 4.9 13.1 1.0 CA A:GLU170 5.0 15.4 1.0

A.J.Mitchell, N.P.Dunham, R.J.Martinie, J.A.Bergman, C.J.Pollock, K.Hu, B.D.Allen, W.C.Chang, A.Silakov, J.M.Bollinger, C.Krebs, A.K.Boal. Visualizing the Reaction Cycle in An Iron(II)- and 2-(Oxo)-Glutarate-Dependent Hydroxylase. J. Am. Chem. Soc. V. 139 13830 2017.
ISSN: ESSN 1520-5126
PubMed: 28823155
DOI: 10.1021/JACS.7B07374