Iron in PDB 6alp: Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate

All present enzymatic activity of Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate:
1.14.11.41;

The structure of Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate, PDB code: 6alp was solved by A.J.Mitchell, N.P.Dunham, A.K.Boal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	59.42 / 1.99
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	80.770, 66.836, 62.925, 90.00, 109.21, 90.00
R / Rfree (%)	21.7 / 25.8

The binding sites of Iron atom in the Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate (pdb code 6alp). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate, PDB code: 6alp:

Iron binding site 1 out of 1 in the Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Vioc L-Arginine Hydroxylase Bound to Fe(II), 3S-Hydroxy-L-Arginine, and Succinate within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe401 b:21.7 occ:1.00 OE1 A:GLU170 2.0 20.5 1.0 OB A:ZZU403 2.3 23.6 1.0 O4 A:SIN402 2.3 21.1 1.0 O3 A:SIN402 2.4 21.0 1.0 NE2 A:HIS316 2.6 21.2 1.0 NE2 A:HIS168 2.6 22.1 1.0 C4 A:SIN402 2.7 21.1 1.0 CD A:GLU170 3.0 20.5 1.0 OE2 A:GLU170 3.3 20.4 1.0 CE1 A:HIS316 3.3 21.2 1.0 CB A:ZZU403 3.4 23.6 1.0 CD2 A:HIS316 3.5 21.2 1.0 CD2 A:HIS168 3.6 22.1 1.0 CE1 A:HIS168 3.6 22.1 1.0 NH1 A:ARG334 4.1 21.2 1.0 CG A:ZZU403 4.1 24.0 1.0 C3 A:SIN402 4.2 21.0 1.0 CG A:GLU170 4.3 20.5 1.0 ND1 A:HIS316 4.4 21.2 1.0 CG A:HIS316 4.5 21.1 1.0 CA A:ZZU403 4.6 23.4 1.0 CB A:GLU170 4.7 20.4 1.0 ND1 A:HIS168 4.7 22.0 1.0 CG A:HIS168 4.7 22.0 1.0 N A:ZZU403 4.7 23.4 1.0 CA A:GLU170 4.9 20.4 1.0 C2 A:SIN402 4.9 21.0 1.0 CD2 A:LEU165 5.0 22.5 1.0

A.J.Mitchell, N.P.Dunham, R.J.Martinie, J.A.Bergman, C.J.Pollock, K.Hu, B.D.Allen, W.C.Chang, A.Silakov, J.M.Bollinger, C.Krebs, A.K.Boal. Visualizing the Reaction Cycle in An Iron(II)- and 2-(Oxo)-Glutarate-Dependent Hydroxylase. J. Am. Chem. Soc. V. 139 13830 2017.
ISSN: ESSN 1520-5126
PubMed: 28823155
DOI: 10.1021/JACS.7B07374