Iron in PDB 6c7o: Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form

All present enzymatic activity of Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form:
1.13.11.75;

The structure of Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form, PDB code: 6c7o was solved by P.D.Kiser, W.Shi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.58 / 3.15
Space group	P 3 1 2
Cell size a, b, c (Å), α, β, γ (°)	126.520, 126.520, 159.670, 90.00, 90.00, 120.00
R / Rfree (%)	23.2 / 26.6

The binding sites of Iron atom in the Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form (pdb code 6c7o). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form, PDB code: 6c7o:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:0.2 occ:1.00 NE2 A:HIS238 2.2 0.9 1.0 NE2 A:HIS484 2.2 0.9 1.0 NE2 A:HIS304 2.2 0.0 1.0 NE2 A:HIS183 2.2 0.5 1.0 CD2 A:HIS238 2.7 0.0 1.0 CE1 A:HIS484 2.9 0.3 1.0 CE1 A:HIS183 3.0 0.4 1.0 CD2 A:HIS304 3.0 0.3 1.0 CD2 A:HIS484 3.2 0.9 1.0 CE1 A:HIS304 3.2 0.5 1.0 CD2 A:HIS183 3.3 0.3 1.0 CE1 A:HIS238 3.4 0.2 1.0 CG A:HIS238 4.0 0.7 1.0 ND1 A:HIS484 4.0 1.0 1.0 CG A:HIS484 4.2 0.7 1.0 ND1 A:HIS183 4.2 0.8 1.0 CG A:HIS304 4.2 0.4 1.0 ND1 A:HIS238 4.3 0.7 1.0 ND1 A:HIS304 4.3 0.2 1.0 CG A:HIS183 4.3 0.7 1.0 CG2 A:THR136 4.9 0.3 1.0

Iron binding site 2 out of 2 in the Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of D477G Aco/RPE65 Chimera, Trigonal Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe501 b:1.0 occ:1.00 NE2 B:HIS238 2.2 0.4 1.0 NE2 B:HIS304 2.2 0.8 1.0 NE2 B:HIS484 2.2 0.4 1.0 NE2 B:HIS183 2.2 0.5 1.0 CD2 B:HIS238 2.7 0.0 1.0 CE1 B:HIS484 2.9 0.7 1.0 CE1 B:HIS183 3.0 0.5 1.0 CD2 B:HIS304 3.0 0.9 1.0 CD2 B:HIS484 3.2 0.2 1.0 CE1 B:HIS304 3.2 0.3 1.0 CD2 B:HIS183 3.3 0.1 1.0 CE1 B:HIS238 3.4 0.1 1.0 CG B:HIS238 4.0 0.2 1.0 ND1 B:HIS484 4.0 0.2 1.0 CG B:HIS484 4.2 0.8 1.0 ND1 B:HIS183 4.2 0.2 1.0 CG B:HIS304 4.2 1.0 1.0 ND1 B:HIS238 4.3 0.2 1.0 ND1 B:HIS304 4.3 0.9 1.0 CG B:HIS183 4.3 0.6 1.0 CG2 B:THR136 4.9 0.4 1.0

E.H.Choi, S.Suh, C.L.Sander, C.J.O.Hernandez, E.R.Bulman, N.Khadka, Z.Dong, W.Shi, K.Palczewski, P.D.Kiser. Insights Into the Pathogenesis of Dominant Retinitis Pigmentosa Associated with A D477G Mutation in RPE65. Hum.Mol.Genet. V. 27 2225 2018.
ISSN: ESSN 1460-2083
PubMed: 29659842
DOI: 10.1093/HMG/DDY128