Iron in PDB 6c7p: Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form

All present enzymatic activity of Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form:
1.13.11.75;

The structure of Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form, PDB code: 6c7p was solved by P.D.Kiser, W.Shi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.76 / 2.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	191.431, 56.170, 136.912, 90.00, 133.82, 90.00
R / Rfree (%)	22 / 26

The binding sites of Iron atom in the Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form (pdb code 6c7p). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form, PDB code: 6c7p:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe501 b:77.6 occ:1.00 NE2 B:HIS304 2.3 67.9 1.0 NE2 B:HIS238 2.4 82.6 1.0 O B:HOH626 2.5 49.2 1.0 NE2 B:HIS183 2.5 58.1 1.0 NE2 B:HIS484 2.5 60.2 1.0 CE1 B:HIS183 2.9 59.8 1.0 CE1 B:HIS238 3.1 83.1 1.0 CE1 B:HIS304 3.1 66.8 1.0 CD2 B:HIS484 3.3 59.1 1.0 CD2 B:HIS304 3.5 66.8 1.0 CD2 B:HIS238 3.6 81.5 1.0 CE1 B:HIS484 3.6 59.9 1.0 CD2 B:HIS183 3.8 58.3 1.0 ND1 B:HIS183 4.2 60.2 1.0 ND1 B:HIS304 4.3 65.0 1.0 ND1 B:HIS238 4.3 81.5 1.0 CE2 B:PHE303 4.3 76.2 1.0 CZ B:PHE303 4.5 74.8 1.0 CG B:HIS304 4.5 66.3 1.0 CG B:HIS484 4.5 57.7 1.0 CG2 B:THR136 4.6 63.7 1.0 CG B:HIS238 4.6 79.9 1.0 ND1 B:HIS484 4.6 57.1 1.0 CG B:HIS183 4.7 57.9 1.0

Iron binding site 2 out of 2 in the Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of D477G Aco/RPE65 Chimera, Monoclinic Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:81.5 occ:1.00 NE2 A:HIS484 2.4 64.9 1.0 NE2 A:HIS183 2.5 65.1 1.0 NE2 A:HIS304 2.5 71.8 1.0 NE2 A:HIS238 2.5 0.2 1.0 CE1 A:HIS183 2.9 63.5 1.0 CE1 A:HIS238 3.1 0.7 1.0 CD2 A:HIS484 3.1 62.5 1.0 CE1 A:HIS304 3.2 74.8 1.0 CE1 A:HIS484 3.5 66.4 1.0 CD2 A:HIS304 3.6 72.1 1.0 CD2 A:HIS238 3.7 0.1 1.0 CD2 A:HIS183 3.8 63.6 1.0 ND1 A:HIS183 4.2 63.7 1.0 ND1 A:HIS238 4.4 0.3 1.0 CG A:HIS484 4.4 61.9 1.0 CG2 A:THR136 4.4 63.1 1.0 ND1 A:HIS304 4.5 72.5 1.0 CE2 A:PHE303 4.5 82.9 1.0 ND1 A:HIS484 4.5 64.6 1.0 CZ A:PHE303 4.6 83.2 1.0 CG A:HIS183 4.6 63.7 1.0 CG A:HIS304 4.7 71.6 1.0 CG A:HIS238 4.7 0.6 1.0

E.H.Choi, S.Suh, C.L.Sander, C.J.O.Hernandez, E.R.Bulman, N.Khadka, Z.Dong, W.Shi, K.Palczewski, P.D.Kiser. Insights Into the Pathogenesis of Dominant Retinitis Pigmentosa Associated with A D477G Mutation in RPE65. Hum.Mol.Genet. V. 27 2225 2018.
ISSN: ESSN 1460-2083
PubMed: 29659842
DOI: 10.1093/HMG/DDY128