Iron in PDB 6cp4: P450CAM D251N Mutant

All present enzymatic activity of P450CAM D251N Mutant:
1.14.15.1;

The structure of P450CAM D251N Mutant, PDB code: 6cp4 was solved by H.Li, T.L.Poulos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	106.600, 103.200, 36.500, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 27

The structure of P450CAM D251N Mutant also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Iron atom in the P450CAM D251N Mutant (pdb code 6cp4). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total only one binding site of Iron was determined in the P450CAM D251N Mutant, PDB code: 6cp4:

Iron binding site 1 out of 1 in the P450CAM D251N Mutant


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of P450CAM D251N Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe417 b:13.0 occ:1.00 FE A:HEM417 0.0 13.0 1.0 ND A:HEM417 2.0 13.0 1.0 NB A:HEM417 2.0 11.8 1.0 NC A:HEM417 2.0 13.3 1.0 NA A:HEM417 2.0 12.4 1.0 SG A:CYS357 2.2 15.0 1.0 C1D A:HEM417 3.0 16.6 1.0 C4C A:HEM417 3.0 13.3 1.0 C4B A:HEM417 3.0 12.2 1.0 C1B A:HEM417 3.0 9.7 1.0 C4D A:HEM417 3.1 13.2 1.0 C1C A:HEM417 3.1 13.7 1.0 C4A A:HEM417 3.1 8.7 1.0 C1A A:HEM417 3.1 10.1 1.0 CB A:CYS357 3.3 14.3 1.0 CHD A:HEM417 3.4 13.2 1.0 CHB A:HEM417 3.4 10.3 1.0 CHC A:HEM417 3.4 13.2 1.0 CHA A:HEM417 3.5 12.0 1.0 H A:GLY359 3.7 0.0 1.0 C5 A:CAM422 3.9 15.0 1.0 CA A:CYS357 4.0 16.1 1.0 HHC A:HEM417 4.2 0.0 1.0 HHD A:HEM417 4.2 0.0 1.0 HHB A:HEM417 4.2 0.0 1.0 C2B A:HEM417 4.3 11.5 1.0 C2D A:HEM417 4.3 16.0 1.0 C3C A:HEM417 4.3 16.2 1.0 C3D A:HEM417 4.3 15.4 1.0 C3B A:HEM417 4.3 9.2 1.0 C2C A:HEM417 4.3 15.6 1.0 C3A A:HEM417 4.3 10.3 1.0 C2A A:HEM417 4.4 9.9 1.0 H A:LEU358 4.4 0.0 1.0 C4 A:CAM422 4.4 12.8 1.0 HHA A:HEM417 4.5 0.0 1.0 N A:GLY359 4.6 16.9 1.0 N A:LEU358 4.7 18.8 1.0 C A:CYS357 4.7 17.9 1.0 CA A:GLY359 4.9 15.2 1.0 C9 A:CAM422 5.0 12.9 1.0

M.Vidakovic, S.G.Sligar, H.Li, T.L.Poulos. Understanding the Role of the Essential ASP251 in Cytochrome P450CAM Using Site-Directed Mutagenesis, Crystallography, and Kinetic Solvent Isotope Effect. Biochemistry V. 37 9211 1998.
ISSN: ISSN 0006-2960
PubMed: 9649301
DOI: 10.1021/BI980189F