Iron in PDB 6e6r: 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa

The structure of 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa, PDB code: 6e6r was solved by S.Lovell, H.Wijerathne, K.P.Battaile, H.Yao, Y.Wang, M.Rivera, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.93 / 1.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	22.444, 43.173, 44.321, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 17.8

The binding sites of Iron atom in the 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa (pdb code 6e6r). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa, PDB code: 6e6r:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe101 b:20.6 occ:1.00 FE1 A:FES101 0.0 20.6 1.0 S2 A:FES101 2.2 20.7 1.0 S1 A:FES101 2.2 18.5 1.0 SG A:CYS38 2.4 21.5 1.0 SG A:CYS6 2.6 26.9 1.0 FE2 A:FES101 2.8 18.1 1.0 CB A:CYS6 3.4 23.4 1.0 CB A:CYS38 3.4 24.1 1.0 N A:CYS38 3.5 24.2 1.0 N A:GLY39 3.8 18.4 1.0 CA A:CYS38 3.9 23.2 1.0 C A:CYS38 4.2 21.1 1.0 N A:LYS40 4.4 18.4 1.0 CB A:GLN37 4.4 25.0 1.0 N A:CYS6 4.4 20.8 1.0 SG A:CYS4 4.5 19.6 1.0 CA A:CYS6 4.5 20.5 1.0 C A:GLN37 4.6 24.7 1.0 SG A:CYS41 4.7 18.6 1.0 CA A:GLY39 4.8 18.2 1.0 CA A:GLN37 5.0 23.4 1.0 C A:GLY39 5.0 19.6 1.0 N A:CYS41 5.0 17.1 1.0

Iron binding site 2 out of 2 in the 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of 1.50 A Resolution Structure of the C-Terminally Truncated [2FE-2S] Ferredoxin (Bfd) R26E Mutant From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe101 b:18.1 occ:1.00 FE2 A:FES101 0.0 18.1 1.0 S2 A:FES101 2.2 20.7 1.0 S1 A:FES101 2.2 18.5 1.0 SG A:CYS41 2.3 18.6 1.0 SG A:CYS4 2.4 19.6 1.0 FE1 A:FES101 2.8 20.6 1.0 CB A:CYS4 3.2 18.1 1.0 CB A:CYS41 3.4 16.5 1.0 CA A:CYS4 3.6 18.9 1.0 N A:GLY35 3.9 18.9 1.0 CA A:GLY35 4.1 18.5 1.0 N A:CYS41 4.2 17.1 1.0 C A:CYS4 4.3 19.4 1.0 CB A:CYS6 4.3 23.4 1.0 CA A:CYS41 4.4 17.6 1.0 N A:LEU5 4.4 18.3 1.0 N A:CYS6 4.5 20.8 1.0 SG A:CYS6 4.6 26.9 1.0 SG A:CYS38 4.7 21.5 1.0 N A:GLY39 4.8 18.4 1.0 N A:CYS4 4.9 19.3 1.0 O A:VAL3 4.9 20.6 1.0 N A:LYS40 5.0 18.4 1.0

H.Wijerathne, H.Yao, Y.Wang, S.Lovell, K.P.Battaile, M.Rivera. Bfd, A New Class of [2FE-2S] Protein That Functions in Bacterial Iron Homeostasis, Requires A Structural Anion Binding Site. Biochemistry V. 57 5533 2018.
ISSN: ISSN 1520-4995
PubMed: 30183257
DOI: 10.1021/ACS.BIOCHEM.8B00823