Iron in PDB 6fsk: F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus

The structure of F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus, PDB code: 6fsk was solved by A.Romero, I.Davo-Siguero, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	70.22 / 1.56
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	62.717, 117.575, 140.434, 90.00, 90.00, 90.00
R / Rfree (%)	16.2 / 19.9

The binding sites of Iron atom in the F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus (pdb code 6fsk). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus, PDB code: 6fsk:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe501 b:9.0 occ:1.00 FE A:HEM501 0.0 9.0 1.0 NA A:HEM501 2.0 9.4 1.0 ND A:HEM501 2.0 8.4 1.0 NC A:HEM501 2.1 8.6 1.0 NB A:HEM501 2.1 8.6 1.0 NE2 A:HIS334 2.2 11.0 1.0 O A:HOH974 2.9 29.5 1.0 C4D A:HEM501 3.0 9.3 1.0 C1D A:HEM501 3.0 8.4 1.0 C4B A:HEM501 3.1 8.5 1.0 C1A A:HEM501 3.1 9.3 1.0 C4A A:HEM501 3.1 9.1 1.0 C1B A:HEM501 3.1 10.3 1.0 C4C A:HEM501 3.1 7.9 1.0 C1C A:HEM501 3.1 8.6 1.0 CE1 A:HIS334 3.1 10.3 1.0 CD2 A:HIS334 3.2 9.6 1.0 CHA A:HEM501 3.4 9.9 1.0 CHC A:HEM501 3.4 8.8 1.0 CHB A:HEM501 3.4 8.8 1.0 CHD A:HEM501 3.5 8.6 1.0 C2C A:HEM501 4.3 9.2 1.0 C2B A:HEM501 4.3 10.5 1.0 C3D A:HEM501 4.3 8.9 1.0 ND1 A:HIS334 4.3 9.4 1.0 C2A A:HEM501 4.3 9.8 1.0 C3A A:HEM501 4.3 10.0 1.0 C3C A:HEM501 4.3 9.2 1.0 C2D A:HEM501 4.3 8.4 1.0 C3B A:HEM501 4.3 8.5 1.0 NH1 A:ARG360 4.3 10.2 1.0 CG A:HIS334 4.4 8.9 1.0 CD A:ARG360 4.8 11.5 1.0

Iron binding site 2 out of 2 in the F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of F194Y Mutant of the Dye-Decolorizing Peroxidase (Dyp) From Pleurotus Ostreatus within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe501 b:11.1 occ:1.00 FE B:HEM501 0.0 11.1 1.0 ND B:HEM501 2.0 10.7 1.0 NA B:HEM501 2.0 12.6 1.0 NB B:HEM501 2.1 11.9 1.0 NC B:HEM501 2.1 12.3 1.0 NE2 B:HIS334 2.2 11.5 1.0 O B:HOH956 3.0 31.5 1.0 C1D B:HEM501 3.0 11.0 1.0 C4D B:HEM501 3.0 12.3 1.0 C4A B:HEM501 3.0 12.0 1.0 C1B B:HEM501 3.0 11.8 1.0 C4C B:HEM501 3.1 10.9 1.0 C4B B:HEM501 3.1 11.2 1.0 C1A B:HEM501 3.1 11.0 1.0 C1C B:HEM501 3.1 11.5 1.0 CE1 B:HIS334 3.1 10.3 1.0 CD2 B:HIS334 3.2 9.6 1.0 CHB B:HEM501 3.4 12.4 1.0 CHD B:HEM501 3.4 11.3 1.0 CHA B:HEM501 3.4 10.8 1.0 CHC B:HEM501 3.5 10.5 1.0 C3D B:HEM501 4.3 11.4 1.0 C2A B:HEM501 4.3 11.2 1.0 C2B B:HEM501 4.3 11.1 1.0 ND1 B:HIS334 4.3 10.0 1.0 C2D B:HEM501 4.3 11.9 1.0 C3A B:HEM501 4.3 11.7 1.0 C3B B:HEM501 4.3 10.5 1.0 C2C B:HEM501 4.3 10.3 1.0 NH1 B:ARG360 4.3 15.2 1.0 CG B:HIS334 4.3 9.9 1.0 C3C B:HEM501 4.3 11.3 1.0 CD B:ARG360 4.9 14.1 1.0

E.Fernandez-Fueyo, I.Davo-Siguero, D.Almendral, D.Linde, M.C.Baratto, R.Pogni, A.Romero, V.Guallar, A.T.Martinez. Description of A Non-Canonical Mn(II)-Oxidation Site in Peroxidases Acs Catalysis 2018.
ISSN: ESSN 2155-5435
DOI: 10.1021/ACSCATAL.8B02306