Atomistry » Iron » PDB 6i95-6j55 » 6j27
Atomistry »
  Iron »
    PDB 6i95-6j55 »
      6j27 »

Iron in PDB 6j27: Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine

Enzymatic activity of Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine

All present enzymatic activity of Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine:
2.5.1.128;

Protein crystallography data

The structure of Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine, PDB code: 6j27 was solved by E.Mizohata, M.Toyoda, J.Fujita, T.Inoue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 1.66
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 69.570, 158.606, 71.936, 90.00, 118.95, 90.00
R / Rfree (%) 16.7 / 20.6

Iron Binding Sites:

The binding sites of Iron atom in the Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine (pdb code 6j27). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine, PDB code: 6j27:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in 6j27

Go back to Iron Binding Sites List in 6j27
Iron binding site 1 out of 2 in the Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe401

b:26.3
occ:0.90
 SG B:CYS100 2.3 31.6 1.0
SG A:CYS100 2.3 32.1 1.0
SG B:CYS97 2.3 34.0 1.0
SG A:CYS97 2.4 34.2 1.0
CB B:CYS97 3.2 33.7 1.0
CB A:CYS97 3.2 29.7 1.0
CB B:CYS100 3.3 29.9 1.0
CB A:CYS100 3.4 37.7 1.0
N B:CYS100 3.8 34.7 1.0
N A:CYS100 3.8 39.1 1.0
CA B:CYS100 4.2 31.9 1.0
CA A:CYS100 4.2 37.8 1.0
CB B:ALA99 4.3 43.4 1.0
CB A:ALA99 4.4 41.5 1.0
CA B:GLY104 4.6 28.5 1.0
C B:ALA99 4.6 40.3 1.0
CA A:CYS97 4.6 33.3 1.0
CA B:CYS97 4.6 34.4 1.0
C A:ALA99 4.6 46.6 1.0
O A:CYS97 4.6 35.6 1.0
N B:GLY104 4.7 28.9 1.0
CA A:GLY104 4.7 27.2 1.0
C A:CYS97 4.8 35.7 1.0
CA B:ALA99 4.8 41.0 1.0
N A:GLY104 4.8 26.3 1.0
C B:CYS97 4.8 37.4 1.0
CA A:ALA99 4.8 41.6 1.0
O B:CYS97 4.9 31.3 1.0
N A:ALA99 4.9 42.2 1.0
N B:ALA99 4.9 39.7 1.0

Iron binding site 2 out of 2 in 6j27

Go back to Iron Binding Sites List in 6j27
Iron binding site 2 out of 2 in the Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of the Branched-Chain Polyamine Synthase From Thermus Thermophilus (Tth-Bpsa) in Complex with N4- Aminopropylspermidine and 5'-Methylthioadenosine within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe401

b:22.1
occ:0.90
 SG C:CYS97 2.3 28.8 1.0
SG D:CYS100 2.3 25.6 1.0
SG D:CYS97 2.3 29.1 1.0
SG C:CYS100 2.3 25.9 1.0
CB D:CYS97 3.1 25.8 1.0
CB C:CYS97 3.1 28.7 1.0
CB C:CYS100 3.4 27.7 1.0
CB D:CYS100 3.4 27.5 1.0
N C:CYS100 3.8 31.0 1.0
N D:CYS100 3.9 30.0 1.0
CA C:CYS100 4.2 28.0 1.0
CA D:CYS100 4.3 27.6 1.0
CB C:ALA99 4.4 35.5 1.0
CB D:ALA99 4.5 38.6 1.0
CA D:CYS97 4.5 25.6 1.0
CA C:CYS97 4.6 27.0 1.0
CA C:GLY104 4.6 24.6 1.0
C C:ALA99 4.7 35.0 1.0
C D:ALA99 4.7 34.7 1.0
O D:CYS97 4.7 28.4 1.0
CA D:GLY104 4.7 23.6 1.0
N C:GLY104 4.8 23.5 1.0
N D:GLY104 4.8 22.8 1.0
C D:CYS97 4.8 30.3 1.0
O C:CYS97 4.8 34.1 1.0
C C:CYS97 4.8 32.6 1.0
N D:ALA99 4.9 32.8 1.0
CA C:ALA99 4.9 36.2 1.0
CA D:ALA99 4.9 34.1 1.0
N C:ALA99 5.0 35.6 1.0

Reference:

R.Hidese, M.Toyoda, K.I.Yoshino, W.Fukuda, G.A.Wihardja, S.Kimura, J.Fujita, M.Niitsu, T.Oshima, T.Imanaka, E.Mizohata, S.Fujiwara. The C-Terminal Flexible Region of Branched-Chain Polyamine Synthase Facilitates Substrate Specificity and Catalysis. Febs J. V. 286 3926 2019.
ISSN: ISSN 1742-464X
PubMed: 31162806
DOI: 10.1111/FEBS.14949
Page generated: Tue Aug 6 23:12:16 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy