Iron in PDB 6j7a: Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA)

All present enzymatic activity of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA):
1.14.14.18; 1.6.2.4;

The structure of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA), PDB code: 6j7a was solved by M.Sugishima, H.Sato, K.Wada, K.Yamamoto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.48 / 3.27
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	82.686, 160.191, 188.832, 90.00, 90.00, 90.00
R / Rfree (%)	22.8 / 24.6

The binding sites of Iron atom in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA) (pdb code 6j7a). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA), PDB code: 6j7a:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA) within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe901 b:56.2 occ:1.00 FE A:HEM901 0.0 56.2 1.0 ND A:HEM901 1.9 47.4 1.0 NA A:HEM901 2.0 54.2 1.0 NC A:HEM901 2.1 49.6 1.0 NB A:HEM901 2.1 50.6 1.0 NE2 A:HIS25 2.2 62.6 1.0 C1D A:HEM901 2.9 45.2 1.0 C4D A:HEM901 2.9 44.0 1.0 C1A A:HEM901 3.0 52.6 1.0 C4C A:HEM901 3.0 45.3 1.0 CE1 A:HIS25 3.0 72.2 1.0 C4B A:HEM901 3.1 49.0 1.0 C4A A:HEM901 3.1 53.3 1.0 C1C A:HEM901 3.1 47.0 1.0 C1B A:HEM901 3.1 52.0 1.0 CD2 A:HIS25 3.2 67.1 1.0 CHD A:HEM901 3.3 41.9 1.0 CHA A:HEM901 3.4 46.3 1.0 CHC A:HEM901 3.5 47.1 1.0 CHB A:HEM901 3.5 50.6 1.0 C2D A:HEM901 4.1 41.2 1.0 C3D A:HEM901 4.1 41.2 1.0 ND1 A:HIS25 4.2 70.1 1.0 C2A A:HEM901 4.2 56.5 1.0 C3C A:HEM901 4.3 43.6 1.0 C3A A:HEM901 4.3 52.7 1.0 C2C A:HEM901 4.3 44.6 1.0 CG A:HIS25 4.3 64.9 1.0 C2B A:HEM901 4.3 49.5 1.0 C3B A:HEM901 4.3 48.5 1.0 CA A:GLY139 4.4 30.1 1.0 CB A:SER142 4.6 40.1 1.0 O A:GLY139 4.7 38.8 1.0

Iron binding site 2 out of 2 in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (17AA) within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe901 b:51.1 occ:1.00 FE B:HEM901 0.0 51.1 1.0 ND B:HEM901 1.9 47.5 1.0 NA B:HEM901 2.0 55.6 1.0 NC B:HEM901 2.1 47.5 1.0 NB B:HEM901 2.1 57.9 1.0 NE2 B:HIS25 2.2 25.8 1.0 C1D B:HEM901 2.9 46.4 1.0 C4D B:HEM901 2.9 47.6 1.0 C1A B:HEM901 3.0 58.4 1.0 C4C B:HEM901 3.0 47.5 1.0 CE1 B:HIS25 3.0 27.1 1.0 C4B B:HEM901 3.1 56.6 1.0 C4A B:HEM901 3.1 59.1 1.0 C1C B:HEM901 3.1 49.4 1.0 C1B B:HEM901 3.1 59.9 1.0 CD2 B:HIS25 3.3 25.6 1.0 CHD B:HEM901 3.3 46.5 1.0 CHA B:HEM901 3.4 52.5 1.0 CHC B:HEM901 3.5 53.7 1.0 CHB B:HEM901 3.5 64.7 1.0 C2D B:HEM901 4.1 45.2 1.0 C3D B:HEM901 4.1 44.7 1.0 C2A B:HEM901 4.2 60.6 1.0 ND1 B:HIS25 4.2 27.8 1.0 C3C B:HEM901 4.2 46.4 1.0 C3A B:HEM901 4.2 60.0 1.0 C2C B:HEM901 4.3 46.4 1.0 C2B B:HEM901 4.3 56.7 1.0 C3B B:HEM901 4.3 54.1 1.0 CG B:HIS25 4.4 26.9 1.0 CA B:GLY139 4.5 34.1 1.0 CB B:SER142 4.6 49.0 1.0 O B:GLY139 4.7 31.5 1.0

M.Sugishima, H.Sato, K.Wada, K.Yamamoto. Crystal Structure of A Nadph-Cytochrome P450 Oxidoreductase (Cypor) and Heme Oxygenase 1 Fusion Protein Implies A Conformational Change in Cypor Upon Nadph/Nadp+Binding. Febs Lett. V. 593 868 2019.
ISSN: ISSN 0014-5793
PubMed: 30883732
DOI: 10.1002/1873-3468.13360