Iron in PDB 6j7i: Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA)

All present enzymatic activity of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA):
1.14.14.18; 1.6.2.4;

The structure of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA), PDB code: 6j7i was solved by M.Sugishima, H.Sato, K.Wada, K.Yamamoto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.04 / 3.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	82.771, 158.101, 188.515, 90.00, 90.00, 90.00
R / Rfree (%)	19.2 / 24.1

The binding sites of Iron atom in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA) (pdb code 6j7i). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA), PDB code: 6j7i:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA) within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe901 b:18.6 occ:1.00 FE A:HEM901 0.0 18.6 1.0 ND A:HEM901 1.9 20.1 1.0 NA A:HEM901 2.0 30.7 1.0 NE2 A:HIS25 2.1 26.1 1.0 NC A:HEM901 2.1 22.6 1.0 NB A:HEM901 2.1 23.4 1.0 C1D A:HEM901 2.9 7.1 1.0 C4D A:HEM901 2.9 18.8 1.0 CD2 A:HIS25 2.9 23.1 1.0 C4C A:HEM901 3.0 19.9 1.0 C1A A:HEM901 3.0 32.2 1.0 C4B A:HEM901 3.0 23.2 1.0 C4A A:HEM901 3.1 30.0 1.0 C1C A:HEM901 3.1 18.7 1.0 C1B A:HEM901 3.1 29.9 1.0 CE1 A:HIS25 3.1 28.7 1.0 CHD A:HEM901 3.3 11.1 1.0 CHA A:HEM901 3.4 22.1 1.0 CHC A:HEM901 3.5 18.6 1.0 CHB A:HEM901 3.5 34.2 1.0 C2D A:HEM901 4.1 14.4 1.0 CG A:HIS25 4.1 24.7 1.0 C3D A:HEM901 4.1 17.0 1.0 ND1 A:HIS25 4.2 26.1 1.0 C2A A:HEM901 4.2 29.2 1.0 C3C A:HEM901 4.2 22.5 1.0 C3A A:HEM901 4.3 22.8 1.0 C2C A:HEM901 4.3 14.5 1.0 C2B A:HEM901 4.3 19.8 1.0 C3B A:HEM901 4.3 27.1 1.0 CA A:GLY139 4.8 32.1 1.0 CB A:SER142 4.9 31.8 1.0

Iron binding site 2 out of 2 in the Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA)


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Fusion Protein of Heme Oxygenase-1 and Nadph Cytochrome P450 Reductase (15AA) within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe901 b:39.6 occ:1.00 FE B:HEM901 0.0 39.6 1.0 ND B:HEM901 1.9 40.0 1.0 NE2 B:HIS25 2.0 28.2 1.0 NA B:HEM901 2.0 41.0 1.0 NC B:HEM901 2.1 35.8 1.0 NB B:HEM901 2.1 47.8 1.0 C1D B:HEM901 2.9 37.0 1.0 C4D B:HEM901 2.9 38.3 1.0 CD2 B:HIS25 3.0 23.5 1.0 CE1 B:HIS25 3.0 31.3 1.0 C1A B:HEM901 3.0 41.5 1.0 C4C B:HEM901 3.0 36.0 1.0 C4B B:HEM901 3.1 45.4 1.0 C4A B:HEM901 3.1 47.0 1.0 C1B B:HEM901 3.1 48.7 1.0 C1C B:HEM901 3.1 37.1 1.0 CHD B:HEM901 3.3 38.4 1.0 CHA B:HEM901 3.4 37.2 1.0 CHC B:HEM901 3.5 39.3 1.0 CHB B:HEM901 3.5 52.0 1.0 ND1 B:HIS25 4.1 30.9 1.0 CG B:HIS25 4.1 29.7 1.0 C2D B:HEM901 4.1 28.8 1.0 C3D B:HEM901 4.1 34.4 1.0 C2A B:HEM901 4.2 43.5 1.0 C3A B:HEM901 4.2 38.6 1.0 C3C B:HEM901 4.2 39.7 1.0 C2C B:HEM901 4.3 41.0 1.0 C2B B:HEM901 4.3 46.8 1.0 C3B B:HEM901 4.3 44.4 1.0 CA B:GLY139 4.9 32.0 1.0

M.Sugishima, H.Sato, K.Wada, K.Yamamoto. Crystal Structure of A Nadph-Cytochrome P450 Oxidoreductase (Cypor) and Heme Oxygenase 1 Fusion Protein Implies A Conformational Change in Cypor Upon Nadph/Nadp+Binding. Febs Lett. V. 593 868 2019.
ISSN: ISSN 0014-5793
PubMed: 30883732
DOI: 10.1002/1873-3468.13360