Iron in PDB 6lsv: Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja

The structure of Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja, PDB code: 6lsv was solved by X.Zhang, D.Wang, J.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.33 / 2.65
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	153.476, 74.416, 75.488, 90, 110.04, 90
R / Rfree (%)	19.5 / 23.4

The binding sites of Iron atom in the Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja (pdb code 6lsv). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja, PDB code: 6lsv:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe403 b:56.8 occ:1.00 O1 A:AKG401 2.0 27.1 1.0 NE2 A:HIS244 2.4 28.4 1.0 OD1 A:ASP246 2.5 30.1 1.0 NE2 A:HIS301 2.6 41.4 1.0 C1 A:AKG401 2.6 34.6 1.0 CE1 A:HIS244 3.0 27.1 1.0 O5 A:AKG401 3.2 41.5 1.0 CD2 A:HIS301 3.2 36.3 1.0 O2 A:AKG401 3.3 45.5 1.0 C2 A:AKG401 3.4 40.3 1.0 CG A:ASP246 3.4 29.6 1.0 CD2 A:HIS244 3.6 29.4 1.0 OD2 A:ASP246 3.6 34.1 1.0 CE1 A:HIS301 3.8 37.2 1.0 C14 A:JAA402 4.1 28.8 1.0 ND1 A:HIS244 4.2 26.0 1.0 C15 A:JAA402 4.3 26.1 1.0 CG A:HIS301 4.5 34.8 1.0 CG A:HIS244 4.5 24.1 1.0 C13 A:JAA402 4.6 24.0 1.0 ND1 A:HIS301 4.7 34.0 1.0 CB A:ASP246 4.8 25.1 1.0 CD2 A:LEU241 4.8 30.1 1.0 C3 A:AKG401 4.9 29.8 1.0 CE1 A:PHE317 4.9 23.4 1.0

Iron binding site 2 out of 2 in the Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of JOX2 in Complex with 2OG, Fe, and Ja within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe403 b:53.3 occ:1.00 OD1 B:ASP246 2.4 30.9 1.0 NE2 B:HIS301 2.5 39.4 1.0 NE2 B:HIS244 2.5 35.1 1.0 O2 B:AKG401 2.5 29.3 1.0 C1 B:AKG401 2.9 34.9 1.0 CE1 B:HIS244 3.1 26.4 1.0 CD2 B:HIS301 3.1 32.2 1.0 O5 B:AKG401 3.2 39.1 1.0 CG B:ASP246 3.3 29.9 1.0 OD2 B:ASP246 3.4 32.4 1.0 O1 B:AKG401 3.4 48.1 1.0 C2 B:AKG401 3.5 34.3 1.0 CD2 B:HIS244 3.6 30.5 1.0 CE1 B:HIS301 3.6 38.6 1.0 C14 B:JAA402 4.2 30.1 1.0 ND1 B:HIS244 4.3 24.1 1.0 CG B:HIS301 4.4 30.5 1.0 C15 B:JAA402 4.5 37.4 1.0 CG B:HIS244 4.5 25.4 1.0 ND1 B:HIS301 4.6 34.6 1.0 C13 B:JAA402 4.6 30.2 1.0 CB B:ASP246 4.7 24.3 1.0 CD2 B:LEU241 4.9 23.5 1.0 CE1 B:PHE317 4.9 22.7 1.0

X.Zhang, D.Wang, J.Elberse, L.Qi, W.Shi, Y.Peng, R.C.Schuurink, G.Van De Ackerveken, J.Liu. Structure-Guided Analysis of the Arabidopsis Jasmonate-Induced Oxygenase (Jox) 2 Reveals Key Residues of Plant Jox Recognizing Jasmonic Acid Substrate. Mol Plant 2021.
ISSN: ESSN 1752-9867
DOI: 10.1016/J.MOLP.2021.01.017