Iron in PDB 6o7l: Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Enzymatic activity of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
All present enzymatic activity of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling:
1.18.6.1;
Protein crystallography data
The structure of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling, PDB code: 6o7l
was solved by
H.L.Rutledge,
F.A.Tezcan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
49.42 /
2.26
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
80.562,
131.029,
107.616,
90.00,
110.85,
90.00
|
R / Rfree (%)
|
20.8 /
25.4
|
Other elements in 6o7l:
The structure of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling also contains other interesting chemical elements:
Iron Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
20;
Page 3, Binding sites: 21 -
30;
Page 4, Binding sites: 31 -
32;
Binding sites:
The binding sites of Iron atom in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
(pdb code 6o7l). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 32 binding sites of Iron where determined in the
Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling, PDB code: 6o7l:
Jump to Iron binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Iron binding site 1 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 1 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:57.7
occ:1.00
|
FE1
|
A:ICS502
|
0.0
|
57.7
|
1.0
|
S2A
|
A:ICS502
|
2.3
|
41.9
|
1.0
|
S4A
|
A:ICS502
|
2.3
|
45.3
|
1.0
|
S1A
|
A:ICS502
|
2.3
|
45.1
|
1.0
|
SG
|
A:CYS275
|
2.4
|
46.9
|
1.0
|
FE4
|
A:ICS502
|
2.7
|
54.3
|
1.0
|
FE3
|
A:ICS502
|
2.7
|
50.5
|
1.0
|
FE2
|
A:ICS502
|
2.7
|
51.8
|
1.0
|
HB2
|
A:CYS275
|
3.1
|
52.3
|
1.0
|
CB
|
A:CYS275
|
3.4
|
43.6
|
1.0
|
CX
|
A:ICS502
|
3.5
|
44.8
|
1.0
|
HG
|
A:SER278
|
3.5
|
56.8
|
1.0
|
HB3
|
A:LEU358
|
3.6
|
60.0
|
1.0
|
HB3
|
A:SER278
|
3.7
|
60.0
|
1.0
|
HB3
|
A:CYS275
|
3.8
|
52.3
|
1.0
|
HE2
|
A:TYR229
|
3.8
|
52.6
|
1.0
|
OG
|
A:SER278
|
3.9
|
47.3
|
1.0
|
HB2
|
A:LEU358
|
4.0
|
60.0
|
1.0
|
HD22
|
A:LEU358
|
4.1
|
47.9
|
1.0
|
CB
|
A:LEU358
|
4.3
|
49.9
|
1.0
|
CB
|
A:SER278
|
4.3
|
50.0
|
1.0
|
HD23
|
A:LEU358
|
4.4
|
47.9
|
1.0
|
CE2
|
A:TYR229
|
4.5
|
43.8
|
1.0
|
H
|
A:SER278
|
4.5
|
52.0
|
1.0
|
HA
|
A:CYS275
|
4.6
|
59.8
|
1.0
|
CA
|
A:CYS275
|
4.6
|
49.8
|
1.0
|
CD2
|
A:LEU358
|
4.7
|
39.9
|
1.0
|
HB3
|
A:ARG277
|
4.7
|
58.3
|
1.0
|
HD2
|
A:TYR229
|
4.7
|
63.5
|
1.0
|
HH
|
A:TYR229
|
4.7
|
59.3
|
1.0
|
S2B
|
A:ICS502
|
4.8
|
44.9
|
1.0
|
S3A
|
A:ICS502
|
4.8
|
38.0
|
1.0
|
S5A
|
A:ICS502
|
4.8
|
37.4
|
1.0
|
HB2
|
A:SER278
|
4.9
|
60.0
|
1.0
|
H
|
A:LEU358
|
4.9
|
54.2
|
1.0
|
CD2
|
A:TYR229
|
5.0
|
52.9
|
1.0
|
FE6
|
A:ICS502
|
5.0
|
53.1
|
1.0
|
FE7
|
A:ICS502
|
5.0
|
50.4
|
1.0
|
|
Iron binding site 2 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 2 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:51.8
occ:1.00
|
FE2
|
A:ICS502
|
0.0
|
51.8
|
1.0
|
CX
|
A:ICS502
|
2.0
|
44.8
|
1.0
|
S2B
|
A:ICS502
|
2.2
|
44.9
|
1.0
|
S2A
|
A:ICS502
|
2.2
|
41.9
|
1.0
|
S1A
|
A:ICS502
|
2.3
|
45.1
|
1.0
|
FE6
|
A:ICS502
|
2.6
|
53.1
|
1.0
|
FE4
|
A:ICS502
|
2.7
|
54.3
|
1.0
|
FE1
|
A:ICS502
|
2.7
|
57.7
|
1.0
|
FE3
|
A:ICS502
|
2.7
|
50.5
|
1.0
|
HZ
|
A:PHE381
|
3.3
|
52.6
|
1.0
|
FE5
|
A:ICS502
|
3.7
|
55.0
|
1.0
|
FE7
|
A:ICS502
|
3.7
|
50.4
|
1.0
|
S4A
|
A:ICS502
|
3.9
|
45.3
|
1.0
|
HG11
|
A:VAL70
|
3.9
|
57.5
|
1.0
|
HE2
|
A:HIS195
|
4.0
|
54.5
|
1.0
|
HE1
|
A:PHE381
|
4.0
|
57.2
|
1.0
|
CZ
|
A:PHE381
|
4.1
|
43.8
|
1.0
|
HB3
|
A:SER278
|
4.1
|
60.0
|
1.0
|
HE1
|
A:HIS195
|
4.1
|
56.2
|
1.0
|
S1B
|
A:ICS502
|
4.2
|
37.0
|
1.0
|
S3B
|
A:ICS502
|
4.2
|
34.8
|
1.0
|
HG13
|
A:VAL70
|
4.3
|
57.5
|
1.0
|
NE2
|
A:HIS195
|
4.3
|
45.4
|
1.0
|
CE1
|
A:HIS195
|
4.4
|
46.9
|
1.0
|
CE1
|
A:PHE381
|
4.5
|
47.6
|
1.0
|
S3A
|
A:ICS502
|
4.5
|
38.0
|
1.0
|
HG21
|
A:VAL70
|
4.5
|
56.1
|
1.0
|
S5A
|
A:ICS502
|
4.5
|
37.4
|
1.0
|
HH12
|
A:ARG96
|
4.6
|
55.1
|
1.0
|
CG1
|
A:VAL70
|
4.6
|
47.9
|
1.0
|
H
|
A:GLY357
|
4.7
|
57.8
|
1.0
|
HH11
|
A:ARG96
|
4.7
|
55.1
|
1.0
|
HG22
|
A:VAL70
|
4.7
|
56.1
|
1.0
|
SG
|
A:CYS275
|
4.7
|
46.9
|
1.0
|
HA3
|
A:GLY356
|
4.9
|
53.7
|
1.0
|
CB
|
A:SER278
|
4.9
|
50.0
|
1.0
|
NH1
|
A:ARG96
|
5.0
|
45.9
|
1.0
|
|
Iron binding site 3 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 3 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:50.5
occ:1.00
|
FE3
|
A:ICS502
|
0.0
|
50.5
|
1.0
|
CX
|
A:ICS502
|
2.0
|
44.8
|
1.0
|
S5A
|
A:ICS502
|
2.2
|
37.4
|
1.0
|
S4A
|
A:ICS502
|
2.3
|
45.3
|
1.0
|
S2A
|
A:ICS502
|
2.3
|
41.9
|
1.0
|
FE7
|
A:ICS502
|
2.6
|
50.4
|
1.0
|
FE4
|
A:ICS502
|
2.6
|
54.3
|
1.0
|
FE1
|
A:ICS502
|
2.7
|
57.7
|
1.0
|
FE2
|
A:ICS502
|
2.7
|
51.8
|
1.0
|
HH12
|
A:ARG96
|
3.6
|
55.1
|
1.0
|
FE6
|
A:ICS502
|
3.7
|
53.1
|
1.0
|
FE5
|
A:ICS502
|
3.7
|
55.0
|
1.0
|
HH11
|
A:ARG96
|
3.7
|
55.1
|
1.0
|
S1A
|
A:ICS502
|
3.9
|
45.1
|
1.0
|
HD2
|
A:TYR229
|
3.9
|
63.5
|
1.0
|
NH1
|
A:ARG96
|
4.0
|
45.9
|
1.0
|
S3B
|
A:ICS502
|
4.2
|
34.8
|
1.0
|
S4B
|
A:ICS502
|
4.2
|
36.5
|
1.0
|
CD2
|
A:TYR229
|
4.3
|
52.9
|
1.0
|
HE2
|
A:TYR229
|
4.3
|
52.6
|
1.0
|
S2B
|
A:ICS502
|
4.5
|
44.9
|
1.0
|
CE2
|
A:TYR229
|
4.5
|
43.8
|
1.0
|
S3A
|
A:ICS502
|
4.5
|
38.0
|
1.0
|
HG13
|
A:VAL70
|
4.7
|
57.5
|
1.0
|
HB2
|
A:CYS275
|
4.7
|
52.3
|
1.0
|
HB2
|
A:TYR229
|
4.8
|
56.7
|
1.0
|
SG
|
A:CYS275
|
4.9
|
46.9
|
1.0
|
|
Iron binding site 4 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 4 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:54.3
occ:1.00
|
FE4
|
A:ICS502
|
0.0
|
54.3
|
1.0
|
CX
|
A:ICS502
|
2.0
|
44.8
|
1.0
|
S3A
|
A:ICS502
|
2.2
|
38.0
|
1.0
|
S1A
|
A:ICS502
|
2.3
|
45.1
|
1.0
|
S4A
|
A:ICS502
|
2.3
|
45.3
|
1.0
|
FE5
|
A:ICS502
|
2.6
|
55.0
|
1.0
|
FE3
|
A:ICS502
|
2.6
|
50.5
|
1.0
|
FE1
|
A:ICS502
|
2.7
|
57.7
|
1.0
|
FE2
|
A:ICS502
|
2.7
|
51.8
|
1.0
|
H
|
A:GLY357
|
3.3
|
57.8
|
1.0
|
HB3
|
A:LEU358
|
3.3
|
60.0
|
1.0
|
H
|
A:LEU358
|
3.5
|
54.2
|
1.0
|
FE7
|
A:ICS502
|
3.7
|
50.4
|
1.0
|
FE6
|
A:ICS502
|
3.7
|
53.1
|
1.0
|
H
|
A:ARG359
|
3.8
|
54.3
|
1.0
|
S2A
|
A:ICS502
|
3.8
|
41.9
|
1.0
|
N
|
A:LEU358
|
4.1
|
45.1
|
1.0
|
N
|
A:GLY357
|
4.1
|
48.1
|
1.0
|
HD2
|
A:ARG359
|
4.1
|
47.9
|
1.0
|
HZ
|
A:PHE381
|
4.1
|
52.6
|
1.0
|
HA3
|
A:GLY356
|
4.2
|
53.7
|
1.0
|
CB
|
A:LEU358
|
4.2
|
49.9
|
1.0
|
S4B
|
A:ICS502
|
4.2
|
36.5
|
1.0
|
S1B
|
A:ICS502
|
4.3
|
37.0
|
1.0
|
HB2
|
A:LEU358
|
4.4
|
60.0
|
1.0
|
S5A
|
A:ICS502
|
4.5
|
37.4
|
1.0
|
S2B
|
A:ICS502
|
4.5
|
44.9
|
1.0
|
H
|
A:GLY356
|
4.6
|
51.0
|
1.0
|
HB2
|
A:ARG359
|
4.6
|
52.9
|
1.0
|
N
|
A:ARG359
|
4.6
|
45.2
|
1.0
|
HD23
|
A:LEU358
|
4.6
|
47.9
|
1.0
|
HA3
|
A:GLY357
|
4.7
|
58.1
|
1.0
|
CA
|
A:LEU358
|
4.7
|
51.4
|
1.0
|
CA
|
A:GLY357
|
4.7
|
48.4
|
1.0
|
HG3
|
A:ARG359
|
4.7
|
53.7
|
1.0
|
C
|
A:GLY357
|
4.7
|
53.0
|
1.0
|
HE1
|
A:PHE381
|
4.8
|
57.2
|
1.0
|
SG
|
A:CYS275
|
4.8
|
46.9
|
1.0
|
CA
|
A:GLY356
|
4.9
|
44.7
|
1.0
|
CD
|
A:ARG359
|
4.9
|
39.9
|
1.0
|
CZ
|
A:PHE381
|
4.9
|
43.8
|
1.0
|
C
|
A:GLY356
|
5.0
|
47.5
|
1.0
|
|
Iron binding site 5 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 5 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 5 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:55.0
occ:1.00
|
FE5
|
A:ICS502
|
0.0
|
55.0
|
1.0
|
CX
|
A:ICS502
|
2.0
|
44.8
|
1.0
|
S4B
|
A:ICS502
|
2.2
|
36.5
|
1.0
|
S3A
|
A:ICS502
|
2.3
|
38.0
|
1.0
|
S1B
|
A:ICS502
|
2.3
|
37.0
|
1.0
|
FE4
|
A:ICS502
|
2.6
|
54.3
|
1.0
|
FE6
|
A:ICS502
|
2.6
|
53.1
|
1.0
|
FE7
|
A:ICS502
|
2.6
|
50.4
|
1.0
|
MO1
|
A:ICS502
|
2.7
|
37.9
|
1.0
|
HG22
|
A:ILE355
|
3.3
|
55.2
|
1.0
|
HA3
|
A:GLY356
|
3.3
|
53.7
|
1.0
|
H
|
A:GLY356
|
3.6
|
51.0
|
1.0
|
FE2
|
A:ICS502
|
3.7
|
51.8
|
1.0
|
FE3
|
A:ICS502
|
3.7
|
50.5
|
1.0
|
HD2
|
A:ARG359
|
3.8
|
47.9
|
1.0
|
HE1
|
A:HIS442
|
3.8
|
37.3
|
1.0
|
ND1
|
A:HIS442
|
3.8
|
38.0
|
1.0
|
S3B
|
A:ICS502
|
3.9
|
34.8
|
1.0
|
HZ
|
A:PHE381
|
4.0
|
52.6
|
1.0
|
HG21
|
A:ILE355
|
4.0
|
55.2
|
1.0
|
CE1
|
A:HIS442
|
4.0
|
31.1
|
1.0
|
CG2
|
A:ILE355
|
4.1
|
46.0
|
1.0
|
H
|
A:GLY357
|
4.1
|
57.8
|
1.0
|
N
|
A:GLY356
|
4.2
|
42.5
|
1.0
|
CA
|
A:GLY356
|
4.2
|
44.7
|
1.0
|
S1A
|
A:ICS502
|
4.3
|
45.1
|
1.0
|
S4A
|
A:ICS502
|
4.3
|
45.3
|
1.0
|
S2B
|
A:ICS502
|
4.5
|
44.9
|
1.0
|
S5A
|
A:ICS502
|
4.5
|
37.4
|
1.0
|
HG23
|
A:ILE355
|
4.6
|
55.2
|
1.0
|
HB2
|
A:ARG359
|
4.7
|
52.9
|
1.0
|
HA
|
A:HIS442
|
4.7
|
42.8
|
1.0
|
HH21
|
A:ARG359
|
4.7
|
51.8
|
1.0
|
CG
|
A:HIS442
|
4.7
|
38.5
|
1.0
|
N
|
A:GLY357
|
4.8
|
48.1
|
1.0
|
HA2
|
A:GLY356
|
4.8
|
53.7
|
1.0
|
CD
|
A:ARG359
|
4.8
|
39.9
|
1.0
|
O7
|
A:HCA501
|
4.8
|
38.4
|
1.0
|
CZ
|
A:PHE381
|
4.8
|
43.8
|
1.0
|
NE2
|
A:HIS442
|
4.9
|
41.4
|
1.0
|
HB
|
A:ILE355
|
5.0
|
49.9
|
1.0
|
H
|
A:ARG359
|
5.0
|
54.3
|
1.0
|
H
|
A:LEU358
|
5.0
|
54.2
|
1.0
|
|
Iron binding site 6 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 6 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 6 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:53.1
occ:1.00
|
FE6
|
A:ICS502
|
0.0
|
53.1
|
1.0
|
CX
|
A:ICS502
|
2.0
|
44.8
|
1.0
|
S2B
|
A:ICS502
|
2.2
|
44.9
|
1.0
|
S3B
|
A:ICS502
|
2.2
|
34.8
|
1.0
|
S1B
|
A:ICS502
|
2.2
|
37.0
|
1.0
|
FE2
|
A:ICS502
|
2.6
|
51.8
|
1.0
|
FE7
|
A:ICS502
|
2.6
|
50.4
|
1.0
|
FE5
|
A:ICS502
|
2.6
|
55.0
|
1.0
|
MO1
|
A:ICS502
|
2.7
|
37.9
|
1.0
|
HZ
|
A:PHE381
|
3.2
|
52.6
|
1.0
|
FE3
|
A:ICS502
|
3.7
|
50.5
|
1.0
|
FE4
|
A:ICS502
|
3.7
|
54.3
|
1.0
|
S4B
|
A:ICS502
|
3.8
|
36.5
|
1.0
|
O7
|
A:HCA501
|
3.8
|
38.4
|
1.0
|
HH11
|
A:ARG96
|
4.0
|
55.1
|
1.0
|
HG22
|
A:VAL70
|
4.0
|
56.1
|
1.0
|
CZ
|
A:PHE381
|
4.1
|
43.8
|
1.0
|
HG21
|
A:VAL70
|
4.1
|
56.1
|
1.0
|
S2A
|
A:ICS502
|
4.2
|
41.9
|
1.0
|
HA3
|
A:GLY356
|
4.3
|
53.7
|
1.0
|
HE2
|
A:PHE381
|
4.3
|
47.6
|
1.0
|
S1A
|
A:ICS502
|
4.3
|
45.1
|
1.0
|
O1
|
A:HCA501
|
4.4
|
46.6
|
1.0
|
S5A
|
A:ICS502
|
4.5
|
37.4
|
1.0
|
HE1
|
A:HIS442
|
4.5
|
37.3
|
1.0
|
S3A
|
A:ICS502
|
4.5
|
38.0
|
1.0
|
CG2
|
A:VAL70
|
4.5
|
46.8
|
1.0
|
O5
|
A:HCA501
|
4.6
|
39.3
|
1.0
|
CE2
|
A:PHE381
|
4.6
|
39.6
|
1.0
|
NH1
|
A:ARG96
|
4.6
|
45.9
|
1.0
|
HH12
|
A:ARG96
|
4.7
|
55.1
|
1.0
|
ND1
|
A:HIS442
|
4.7
|
38.0
|
1.0
|
HE2
|
A:HIS195
|
4.8
|
54.5
|
1.0
|
HG11
|
A:VAL70
|
4.9
|
57.5
|
1.0
|
HD2
|
A:ARG96
|
4.9
|
49.4
|
1.0
|
CE1
|
A:HIS442
|
4.9
|
31.1
|
1.0
|
C3
|
A:HCA501
|
5.0
|
43.5
|
1.0
|
H22
|
A:HCA501
|
5.0
|
43.8
|
1.0
|
FE1
|
A:ICS502
|
5.0
|
57.7
|
1.0
|
|
Iron binding site 7 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 7 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 7 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe502
b:50.4
occ:1.00
|
FE7
|
A:ICS502
|
0.0
|
50.4
|
1.0
|
CX
|
A:ICS502
|
2.0
|
44.8
|
1.0
|
S5A
|
A:ICS502
|
2.2
|
37.4
|
1.0
|
S4B
|
A:ICS502
|
2.2
|
36.5
|
1.0
|
S3B
|
A:ICS502
|
2.2
|
34.8
|
1.0
|
FE3
|
A:ICS502
|
2.6
|
50.5
|
1.0
|
FE6
|
A:ICS502
|
2.6
|
53.1
|
1.0
|
FE5
|
A:ICS502
|
2.6
|
55.0
|
1.0
|
MO1
|
A:ICS502
|
2.7
|
37.9
|
1.0
|
HH11
|
A:ARG96
|
2.9
|
55.1
|
1.0
|
HD2
|
A:ARG96
|
3.5
|
49.4
|
1.0
|
NH1
|
A:ARG96
|
3.7
|
45.9
|
1.0
|
O
|
A:HOH637
|
3.7
|
33.4
|
1.0
|
FE4
|
A:ICS502
|
3.7
|
54.3
|
1.0
|
FE2
|
A:ICS502
|
3.7
|
51.8
|
1.0
|
S1B
|
A:ICS502
|
3.8
|
37.0
|
1.0
|
HH12
|
A:ARG96
|
3.9
|
55.1
|
1.0
|
O5
|
A:HCA501
|
3.9
|
39.3
|
1.0
|
S2A
|
A:ICS502
|
4.3
|
41.9
|
1.0
|
S4A
|
A:ICS502
|
4.3
|
45.3
|
1.0
|
CD
|
A:ARG96
|
4.4
|
41.1
|
1.0
|
S2B
|
A:ICS502
|
4.4
|
44.9
|
1.0
|
S3A
|
A:ICS502
|
4.5
|
38.0
|
1.0
|
HD3
|
A:ARG96
|
4.5
|
49.4
|
1.0
|
HG13
|
A:ILE231
|
4.5
|
49.2
|
1.0
|
ND1
|
A:HIS442
|
4.6
|
38.0
|
1.0
|
NH2
|
A:ARG359
|
4.6
|
43.2
|
1.0
|
HH22
|
A:ARG359
|
4.6
|
51.8
|
1.0
|
HD11
|
A:ILE231
|
4.7
|
49.8
|
1.0
|
HA
|
A:HIS442
|
4.7
|
42.8
|
1.0
|
HH21
|
A:ARG359
|
4.7
|
51.8
|
1.0
|
O7
|
A:HCA501
|
4.7
|
38.4
|
1.0
|
HD2
|
A:ARG359
|
4.8
|
47.9
|
1.0
|
HB3
|
A:HIS442
|
4.8
|
45.1
|
1.0
|
CZ
|
A:ARG96
|
4.8
|
44.4
|
1.0
|
CZ
|
A:ARG359
|
4.8
|
49.4
|
1.0
|
HG22
|
A:VAL70
|
4.9
|
56.1
|
1.0
|
C7
|
A:HCA501
|
4.9
|
38.1
|
1.0
|
FE1
|
A:ICS502
|
5.0
|
57.7
|
1.0
|
|
Iron binding site 8 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 8 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 8 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:55.2
occ:1.00
|
FE1
|
B:CLF601
|
0.0
|
55.2
|
1.0
|
S3A
|
B:CLF601
|
2.2
|
43.3
|
1.0
|
S2A
|
B:CLF601
|
2.2
|
44.5
|
1.0
|
S1
|
B:CLF601
|
2.5
|
48.6
|
1.0
|
FE2
|
B:CLF601
|
2.5
|
53.8
|
1.0
|
SG
|
B:CYS95
|
2.5
|
44.0
|
1.0
|
FE4
|
B:CLF601
|
2.6
|
52.5
|
1.0
|
FE3
|
B:CLF601
|
2.7
|
48.8
|
1.0
|
FE8
|
B:CLF601
|
2.9
|
59.8
|
1.0
|
H
|
B:CYS95
|
2.9
|
48.5
|
1.0
|
N
|
B:CYS95
|
3.2
|
40.4
|
1.0
|
HG
|
B:SER92
|
3.3
|
70.2
|
1.0
|
HA
|
B:CYS95
|
3.4
|
51.8
|
1.0
|
CB
|
B:CYS95
|
3.6
|
43.8
|
1.0
|
CA
|
B:CYS95
|
3.6
|
43.2
|
1.0
|
S4A
|
B:CLF601
|
3.7
|
41.0
|
1.0
|
FE5
|
B:CLF601
|
3.8
|
59.2
|
1.0
|
S4B
|
B:CLF601
|
3.8
|
41.2
|
1.0
|
HA3
|
B:GLY94
|
3.9
|
44.4
|
1.0
|
C
|
B:GLY94
|
3.9
|
43.1
|
1.0
|
HB3
|
B:CYS95
|
4.0
|
52.6
|
1.0
|
OG
|
B:SER92
|
4.2
|
58.5
|
1.0
|
O
|
B:HOH756
|
4.3
|
45.5
|
1.0
|
CA
|
B:GLY94
|
4.4
|
37.0
|
1.0
|
HB2
|
B:CYS95
|
4.4
|
52.6
|
1.0
|
HB2
|
B:SER92
|
4.5
|
60.3
|
1.0
|
HB3
|
A:CYS62
|
4.6
|
50.5
|
1.0
|
H
|
B:GLY94
|
4.6
|
47.6
|
1.0
|
SG
|
A:CYS154
|
4.6
|
40.1
|
1.0
|
HA3
|
A:GLY185
|
4.6
|
49.8
|
1.0
|
HD2
|
B:TYR98
|
4.7
|
53.0
|
1.0
|
O
|
B:GLY94
|
4.7
|
38.4
|
1.0
|
N
|
B:GLY94
|
4.7
|
39.7
|
1.0
|
SG
|
A:CYS88
|
4.7
|
43.7
|
1.0
|
SG
|
A:CYS62
|
4.8
|
39.2
|
1.0
|
HB
|
B:THR152
|
4.8
|
51.6
|
1.0
|
O
|
B:SER92
|
4.8
|
43.5
|
1.0
|
CB
|
B:SER92
|
4.9
|
50.2
|
1.0
|
S3B
|
B:CLF601
|
5.0
|
42.1
|
1.0
|
FE6
|
B:CLF601
|
5.0
|
58.1
|
1.0
|
|
Iron binding site 9 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 9 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 9 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:53.8
occ:1.00
|
FE2
|
B:CLF601
|
0.0
|
53.8
|
1.0
|
S4A
|
B:CLF601
|
2.2
|
41.0
|
1.0
|
S2A
|
B:CLF601
|
2.2
|
44.5
|
1.0
|
S1
|
B:CLF601
|
2.4
|
48.6
|
1.0
|
SG
|
A:CYS154
|
2.4
|
40.1
|
1.0
|
FE1
|
B:CLF601
|
2.5
|
55.2
|
1.0
|
FE4
|
B:CLF601
|
2.6
|
52.5
|
1.0
|
FE3
|
B:CLF601
|
2.8
|
48.8
|
1.0
|
HA3
|
A:GLY185
|
3.0
|
49.8
|
1.0
|
HG
|
B:SER92
|
3.1
|
70.2
|
1.0
|
HB2
|
A:CYS154
|
3.1
|
51.6
|
1.0
|
H
|
A:CYS154
|
3.2
|
52.9
|
1.0
|
CB
|
A:CYS154
|
3.4
|
43.0
|
1.0
|
H
|
A:GLY185
|
3.5
|
51.0
|
1.0
|
O
|
B:HOH756
|
3.7
|
45.5
|
1.0
|
S3A
|
B:CLF601
|
3.7
|
43.3
|
1.0
|
OG
|
B:SER92
|
3.8
|
58.5
|
1.0
|
CA
|
A:GLY185
|
3.9
|
41.5
|
1.0
|
N
|
A:CYS154
|
3.9
|
44.0
|
1.0
|
N
|
A:GLY185
|
4.1
|
42.5
|
1.0
|
CA
|
A:CYS154
|
4.1
|
41.9
|
1.0
|
HB3
|
A:CYS154
|
4.2
|
51.6
|
1.0
|
HA
|
A:CYS154
|
4.2
|
50.3
|
1.0
|
FE8
|
B:CLF601
|
4.3
|
59.8
|
1.0
|
SG
|
B:CYS95
|
4.3
|
44.0
|
1.0
|
HB2
|
B:SER92
|
4.4
|
60.3
|
1.0
|
HA2
|
A:GLY185
|
4.5
|
49.8
|
1.0
|
FE5
|
B:CLF601
|
4.5
|
59.2
|
1.0
|
HB3
|
A:GLU153
|
4.5
|
49.4
|
1.0
|
CB
|
B:SER92
|
4.7
|
50.2
|
1.0
|
SG
|
A:CYS88
|
4.7
|
43.7
|
1.0
|
C
|
A:GLY185
|
4.7
|
49.5
|
1.0
|
HA
|
A:GLU153
|
4.8
|
51.8
|
1.0
|
HB2
|
A:CYS62
|
4.8
|
50.5
|
1.0
|
H
|
A:PHE186
|
4.9
|
60.6
|
1.0
|
SG
|
A:CYS62
|
5.0
|
39.2
|
1.0
|
HB3
|
A:CYS62
|
5.0
|
50.5
|
1.0
|
HB3
|
A:TYR64
|
5.0
|
47.5
|
1.0
|
|
Iron binding site 10 out
of 32 in 6o7l
Go back to
Iron Binding Sites List in 6o7l
Iron binding site 10 out
of 32 in the Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 10 of Nitrogenase Mofep Mutant S188A From Azotobacter Vinelandii in the Dithionite Reduced State After Redox Cycling within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Fe601
b:48.8
occ:1.00
|
FE3
|
B:CLF601
|
0.0
|
48.8
|
1.0
|
S3A
|
B:CLF601
|
2.2
|
43.3
|
1.0
|
S2A
|
B:CLF601
|
2.2
|
44.5
|
1.0
|
S4A
|
B:CLF601
|
2.3
|
41.0
|
1.0
|
SG
|
A:CYS62
|
2.3
|
39.2
|
1.0
|
FE4
|
B:CLF601
|
2.7
|
52.5
|
1.0
|
FE1
|
B:CLF601
|
2.7
|
55.2
|
1.0
|
FE2
|
B:CLF601
|
2.8
|
53.8
|
1.0
|
HB3
|
A:CYS62
|
3.0
|
50.5
|
1.0
|
HA3
|
A:GLY185
|
3.0
|
49.8
|
1.0
|
CB
|
A:CYS62
|
3.1
|
42.1
|
1.0
|
HB3
|
A:TYR64
|
3.1
|
47.5
|
1.0
|
HB2
|
A:CYS62
|
3.1
|
50.5
|
1.0
|
HA3
|
B:GLY94
|
3.4
|
44.4
|
1.0
|
HA2
|
A:GLY185
|
3.8
|
49.8
|
1.0
|
CA
|
A:GLY185
|
3.8
|
41.5
|
1.0
|
CB
|
A:TYR64
|
4.0
|
39.6
|
1.0
|
H
|
A:TYR64
|
4.1
|
51.3
|
1.0
|
HD1
|
A:TYR64
|
4.1
|
55.5
|
1.0
|
S1
|
B:CLF601
|
4.2
|
48.6
|
1.0
|
HB2
|
A:TYR64
|
4.2
|
47.5
|
1.0
|
H
|
A:GLY185
|
4.3
|
51.0
|
1.0
|
CA
|
B:GLY94
|
4.3
|
37.0
|
1.0
|
HE2
|
B:TYR98
|
4.4
|
50.4
|
1.0
|
N
|
A:GLY185
|
4.5
|
42.5
|
1.0
|
H
|
B:CYS95
|
4.5
|
48.5
|
1.0
|
CA
|
A:CYS62
|
4.5
|
44.2
|
1.0
|
H
|
A:ALA65
|
4.6
|
43.2
|
1.0
|
CD1
|
A:TYR64
|
4.6
|
46.2
|
1.0
|
C
|
B:GLY94
|
4.6
|
43.1
|
1.0
|
HG
|
B:SER92
|
4.6
|
70.2
|
1.0
|
CG
|
A:TYR64
|
4.7
|
43.1
|
1.0
|
H
|
B:GLY94
|
4.7
|
47.6
|
1.0
|
N
|
B:CYS95
|
4.7
|
40.4
|
1.0
|
H
|
A:CYS62
|
4.7
|
42.5
|
1.0
|
N
|
A:TYR64
|
4.8
|
42.7
|
1.0
|
HB3
|
A:CYS88
|
4.9
|
52.9
|
1.0
|
SG
|
A:CYS154
|
4.9
|
40.1
|
1.0
|
C
|
A:GLY185
|
4.9
|
49.5
|
1.0
|
HA2
|
B:GLY94
|
4.9
|
44.4
|
1.0
|
SG
|
A:CYS88
|
4.9
|
43.7
|
1.0
|
H
|
A:ALA63
|
5.0
|
49.6
|
1.0
|
HD2
|
B:TYR98
|
5.0
|
53.0
|
1.0
|
N
|
B:GLY94
|
5.0
|
39.7
|
1.0
|
HB2
|
B:SER92
|
5.0
|
60.3
|
1.0
|
|
Reference:
H.L.Rutledge,
J.Rittle,
L.M.Williamson,
W.A.Xu,
D.M.Gagnon,
F.A.Tezcan.
Redox-Dependent Metastability of the Nitrogenase P-Cluster. J.Am.Chem.Soc. V. 141 10091 2019.
ISSN: ESSN 1520-5126
PubMed: 31146522
DOI: 10.1021/JACS.9B04555
Page generated: Wed Aug 7 04:08:44 2024
|