Iron in PDB 6s2e: Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon

All present enzymatic activity of Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon:
2.7.7.7;

The binding sites of Iron atom in the Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon (pdb code 6s2e). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon, PDB code: 6s2e:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1401 b:0.8 occ:1.00 FE1 A:SF41401 0.0 0.8 1.0 S4 A:SF41401 2.1 0.8 1.0 S2 A:SF41401 2.1 0.8 1.0 S3 A:SF41401 2.1 0.8 1.0 SG A:CYS677 2.2 0.7 1.0 FE2 A:SF41401 2.5 0.8 1.0 CB A:CYS677 2.5 0.7 1.0 FE3 A:SF41401 2.5 0.8 1.0 FE4 A:SF41401 2.6 0.8 1.0 S1 A:SF41401 3.6 0.8 1.0 CA A:CYS677 3.8 0.7 1.0 CD A:ARG765 4.0 0.9 1.0 C A:CYS677 4.1 0.7 1.0 NH1 A:ARG765 4.1 0.9 1.0 SG A:CYS763 4.4 0.1 1.0 N A:ALA678 4.4 0.8 1.0 SG A:CYS665 4.4 0.8 1.0 O A:CYS677 4.5 0.7 1.0 O A:THR676 4.6 0.7 1.0 CB A:CYS763 4.6 0.1 1.0 CG A:ARG765 4.7 0.9 1.0 SG A:CYS668 4.7 0.3 1.0 O A:ASP669 4.7 0.3 1.0 CB A:ARG765 4.8 0.9 1.0 N A:CYS677 4.8 0.7 1.0 NE A:ARG765 4.9 0.9 1.0 CA A:CYS763 4.9 0.1 1.0 CZ A:ARG765 4.9 0.9 1.0 N A:GLN764 4.9 0.1 1.0

Iron binding site 2 out of 4 in the Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1401 b:0.8 occ:1.00 FE2 A:SF41401 0.0 0.8 1.0 S3 A:SF41401 2.1 0.8 1.0 S1 A:SF41401 2.1 0.8 1.0 S4 A:SF41401 2.2 0.8 1.0 SG A:CYS668 2.3 0.3 1.0 FE4 A:SF41401 2.4 0.8 1.0 FE1 A:SF41401 2.5 0.8 1.0 FE3 A:SF41401 2.6 0.8 1.0 CB A:CYS668 3.5 0.3 1.0 S2 A:SF41401 3.5 0.8 1.0 NH1 A:ARG765 3.6 0.9 1.0 CA A:CYS665 4.3 0.8 1.0 N A:CYS665 4.3 0.8 1.0 CZ A:ARG765 4.3 0.9 1.0 CD2 A:LEU181 4.4 0.9 1.0 SG A:CYS665 4.4 0.8 1.0 SG A:CYS677 4.5 0.7 1.0 CB A:CYS677 4.6 0.7 1.0 O A:CYS668 4.6 0.3 1.0 CA A:CYS668 4.6 0.3 1.0 O A:ASP669 4.6 0.3 1.0 CD A:ARG765 4.7 0.9 1.0 C A:CYS668 4.7 0.3 1.0 SG A:CYS763 4.8 0.1 1.0 NE A:ARG765 4.8 0.9 1.0 CB A:CYS665 4.9 0.8 1.0

Iron binding site 3 out of 4 in the Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1401 b:0.8 occ:1.00 FE3 A:SF41401 0.0 0.8 1.0 S2 A:SF41401 2.1 0.8 1.0 S4 A:SF41401 2.1 0.8 1.0 S1 A:SF41401 2.1 0.8 1.0 SG A:CYS763 2.3 0.1 1.0 FE1 A:SF41401 2.5 0.8 1.0 FE4 A:SF41401 2.5 0.8 1.0 FE2 A:SF41401 2.6 0.8 1.0 CB A:CYS763 3.2 0.1 1.0 S3 A:SF41401 3.7 0.8 1.0 CA A:CYS763 4.0 0.1 1.0 CB A:ASP664 4.4 0.1 1.0 SG A:CYS677 4.5 0.7 1.0 OD2 A:ASP664 4.5 0.1 1.0 SG A:CYS668 4.5 0.3 1.0 SG A:CYS665 4.6 0.8 1.0 CB A:CYS677 4.7 0.7 1.0 N A:CYS665 4.8 0.8 1.0 CG A:ASP664 4.9 0.1 1.0

Iron binding site 4 out of 4 in the Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Cryo-Em Structure of CTF18-1-8 in Complex with the Catalytic Domain of Dna Polymerase Epsilon within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe1401 b:0.8 occ:1.00 FE4 A:SF41401 0.0 0.8 1.0 S2 A:SF41401 2.1 0.8 1.0 S1 A:SF41401 2.1 0.8 1.0 S3 A:SF41401 2.1 0.8 1.0 SG A:CYS665 2.2 0.8 1.0 FE2 A:SF41401 2.4 0.8 1.0 FE3 A:SF41401 2.5 0.8 1.0 FE1 A:SF41401 2.6 0.8 1.0 N A:CYS665 3.2 0.8 1.0 CB A:CYS665 3.3 0.8 1.0 CA A:CYS665 3.4 0.8 1.0 S4 A:SF41401 3.6 0.8 1.0 CB A:CYS677 3.8 0.7 1.0 CB A:ASP664 4.0 0.1 1.0 SG A:CYS668 4.0 0.3 1.0 OD2 A:ASP664 4.1 0.1 1.0 O A:THR676 4.1 0.7 1.0 CG A:ASP664 4.1 0.1 1.0 C A:ASP664 4.2 0.1 1.0 SG A:CYS763 4.4 0.1 1.0 SG A:CYS677 4.5 0.7 1.0 OG1 A:THR676 4.6 0.7 1.0 CA A:ASP664 4.7 0.1 1.0 OD1 A:ASP664 4.7 0.1 1.0 C A:CYS665 4.9 0.8 1.0 C A:THR676 4.9 0.7 1.0 N A:ALA678 5.0 0.8 1.0 CA A:CYS677 5.0 0.7 1.0

K.Stokes, A.Winczura, B.Song, G.De Piccoli, D.B.Grabarczyk. The Leading Strand Clamp Loader CTF18-Rfc Assumes Distinct Roles in Cohesion Establishment and Dna Replication To Be Published.