Iron in PDB 7ae1: Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1

All present enzymatic activity of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1:
2.7.7.6;

The structure of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1 also contains other interesting chemical elements:

Zinc	(Zn)	7 atoms
Magnesium	(Mg)	1 atom

The binding sites of Iron atom in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1 (pdb code 7ae1). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1, PDB code: 7ae1:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:93.5 occ:1.00 FE1 P:SF4401 0.0 93.5 1.0 S4 P:SF4401 2.3 93.5 1.0 S3 P:SF4401 2.3 93.5 1.0 S2 P:SF4401 2.3 93.5 1.0 FE4 P:SF4401 2.7 93.5 1.0 FE3 P:SF4401 2.7 93.5 1.0 FE2 P:SF4401 2.7 93.5 1.0 SG P:CYS290 3.1 95.5 1.0 CB P:CYS290 3.5 95.5 1.0 S1 P:SF4401 3.9 93.5 1.0 CD2 P:TYR309 4.2 96.8 1.0 SG P:CYS307 4.2 97.3 1.0 CA P:CYS287 4.6 96.9 1.0 O P:PRO286 4.7 97.3 1.0 CA P:CYS290 4.8 95.5 1.0 CB P:TYR309 4.8 96.8 1.0 CG P:TYR309 4.9 96.8 1.0 CE2 P:TYR309 4.9 96.8 1.0 N P:CYS287 5.0 96.9 1.0

Iron binding site 2 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:93.5 occ:1.00 FE2 P:SF4401 0.0 93.5 1.0 S1 P:SF4401 2.3 93.5 1.0 S3 P:SF4401 2.3 93.5 1.0 S4 P:SF4401 2.3 93.5 1.0 FE4 P:SF4401 2.7 93.5 1.0 FE3 P:SF4401 2.7 93.5 1.0 FE1 P:SF4401 2.7 93.5 1.0 SG P:CYS307 3.0 97.3 1.0 CB P:CYS307 3.2 97.3 1.0 S2 P:SF4401 3.9 93.5 1.0 CA P:PRO304 4.3 92.1 1.0 SG P:CYS296 4.4 89.3 1.0 CE P:MET310 4.4 98.0 1.0 O P:SER303 4.5 91.8 1.0 CG P:MET310 4.6 98.0 1.0 CA P:CYS307 4.6 97.3 1.0 N P:PRO304 4.7 92.1 1.0 SD P:MET310 4.7 98.0 1.0 C P:SER303 4.9 91.8 1.0 CB P:PRO304 4.9 92.1 1.0

Iron binding site 3 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:93.5 occ:1.00 FE3 P:SF4401 0.0 93.5 1.0 S2 P:SF4401 2.3 93.5 1.0 S4 P:SF4401 2.3 93.5 1.0 S1 P:SF4401 2.3 93.5 1.0 FE2 P:SF4401 2.7 93.5 1.0 FE1 P:SF4401 2.7 93.5 1.0 FE4 P:SF4401 2.7 93.5 1.0 SG P:CYS296 3.1 89.3 1.0 CB P:CYS296 3.3 89.3 1.0 S3 P:SF4401 3.9 93.5 1.0 ND2 O:ASN445 4.1 91.2 1.0 SG P:CYS290 4.6 95.5 1.0 CG O:ASN445 4.8 91.2 1.0 CA P:CYS296 4.8 89.3 1.0

Iron binding site 4 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 1 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:93.5 occ:1.00 FE4 P:SF4401 0.0 93.5 1.0 S1 P:SF4401 2.3 93.5 1.0 S3 P:SF4401 2.3 93.5 1.0 S2 P:SF4401 2.3 93.5 1.0 FE2 P:SF4401 2.7 93.5 1.0 FE1 P:SF4401 2.7 93.5 1.0 FE3 P:SF4401 2.7 93.5 1.0 SG P:CYS287 3.0 96.9 1.0 CE P:MET310 3.7 98.0 1.0 CB P:CYS287 3.8 96.9 1.0 CA P:CYS287 3.8 96.9 1.0 S4 P:SF4401 3.9 93.5 1.0 ND2 O:ASN445 3.9 91.2 1.0 N P:CYS287 4.2 96.9 1.0 CB O:ASN445 4.3 91.2 1.0 CG O:ASN445 4.5 91.2 1.0 C P:PRO286 4.9 97.3 1.0 SD P:MET310 4.9 98.0 1.0

M.Girbig, A.D.Misiaszek, M.K.Vorlaender, A.Lafita, H.Groetsch, F.Baudin, A.Bateman, C.W.Mueller. Cryo-Em Structures of Human Rna Polymerase III in Its Unbound and Transcribing States To Be Published.
DOI: 10.1038/S41594-020-00555-5