Iron in PDB 7ae3: Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3

All present enzymatic activity of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3:
2.7.7.6;

The structure of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3 also contains other interesting chemical elements:

Zinc	(Zn)	7 atoms
Magnesium	(Mg)	1 atom

The binding sites of Iron atom in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3 (pdb code 7ae3). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3, PDB code: 7ae3:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:127.2 occ:1.00 FE1 P:SF4401 0.0 127.2 1.0 S3 P:SF4401 2.3 127.2 1.0 S4 P:SF4401 2.3 127.2 1.0 S2 P:SF4401 2.3 127.2 1.0 FE3 P:SF4401 2.7 127.2 1.0 FE2 P:SF4401 2.7 127.2 1.0 FE4 P:SF4401 2.7 127.2 1.0 SG P:CYS290 3.1 132.1 1.0 CB P:CYS290 3.4 132.1 1.0 S1 P:SF4401 3.9 127.2 1.0 CD2 P:TYR309 4.1 135.8 1.0 SG P:CYS307 4.3 133.7 1.0 O P:PRO286 4.4 134.8 1.0 CB P:TYR309 4.6 135.8 1.0 CA P:CYS290 4.8 132.1 1.0 CG P:TYR309 4.8 135.8 1.0 CA P:CYS287 4.8 134.9 1.0 CE2 P:TYR309 4.8 135.8 1.0 C P:PRO286 5.0 134.8 1.0

Iron binding site 2 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:127.2 occ:1.00 FE2 P:SF4401 0.0 127.2 1.0 S1 P:SF4401 2.3 127.2 1.0 S3 P:SF4401 2.3 127.2 1.0 S4 P:SF4401 2.3 127.2 1.0 FE4 P:SF4401 2.7 127.2 1.0 FE3 P:SF4401 2.7 127.2 1.0 FE1 P:SF4401 2.7 127.2 1.0 SG P:CYS307 3.0 133.7 1.0 CB P:CYS307 3.2 133.7 1.0 S2 P:SF4401 3.9 127.2 1.0 CA P:PRO304 4.3 126.8 1.0 CE P:MET310 4.5 140.2 1.0 O P:SER303 4.5 128.9 1.0 SG P:CYS296 4.6 128.1 1.0 CA P:CYS307 4.6 133.7 1.0 CG P:MET310 4.6 140.2 1.0 SD P:MET310 4.7 140.2 1.0 N P:PRO304 4.8 126.8 1.0 CB P:PRO304 4.9 126.8 1.0 C P:SER303 4.9 128.9 1.0

Iron binding site 3 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:127.2 occ:1.00 FE3 P:SF4401 0.0 127.2 1.0 S2 P:SF4401 2.3 127.2 1.0 S4 P:SF4401 2.3 127.2 1.0 S1 P:SF4401 2.3 127.2 1.0 FE2 P:SF4401 2.7 127.2 1.0 FE1 P:SF4401 2.7 127.2 1.0 FE4 P:SF4401 2.7 127.2 1.0 SG P:CYS296 3.1 128.1 1.0 CB P:CYS296 3.4 128.1 1.0 S3 P:SF4401 3.9 127.2 1.0 ND2 O:ASN445 4.5 131.4 1.0 SG P:CYS290 4.6 132.1 1.0 CA P:CYS296 4.9 128.1 1.0 CG O:ASN445 4.9 131.4 1.0 CD1 P:PHE293 4.9 130.8 1.0

Iron binding site 4 out of 4 in the Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Cryo-Em Structure of Human Rna Polymerase III Elongation Complex 3 within 5.0Å range: probe atom residue distance (Å) B Occ P:Fe401 b:127.2 occ:1.00 FE4 P:SF4401 0.0 127.2 1.0 S1 P:SF4401 2.3 127.2 1.0 S3 P:SF4401 2.3 127.2 1.0 S2 P:SF4401 2.3 127.2 1.0 FE2 P:SF4401 2.7 127.2 1.0 FE1 P:SF4401 2.7 127.2 1.0 FE3 P:SF4401 2.7 127.2 1.0 SG P:CYS287 3.1 134.9 1.0 CE P:MET310 3.5 140.2 1.0 S4 P:SF4401 3.9 127.2 1.0 CB P:CYS287 4.0 134.9 1.0 CA P:CYS287 4.0 134.9 1.0 N P:CYS287 4.4 134.9 1.0 ND2 O:ASN445 4.4 131.4 1.0 CB O:ASN445 4.5 131.4 1.0 CG O:ASN445 4.6 131.4 1.0 SD P:MET310 4.7 140.2 1.0 C P:PRO286 4.8 134.8 1.0 CG P:MET310 4.8 140.2 1.0 O P:PRO286 4.9 134.8 1.0

M.Girbig, A.D.Misiaszek, M.K.Vorlaender, A.Lafita, H.Groetsch, F.Baudin, A.Bateman, C.W.Mueller. Cryo-Em Structures of Human Rna Polymerase III in Its Unbound and Transcribing States To Be Published.
DOI: 10.1038/S41594-020-00555-5