Iron in PDB 7c8n: Crystal Structure of Iscu H106A Variant

The structure of Crystal Structure of Iscu H106A Variant, PDB code: 7c8n was solved by K.Kunichika, Y.Takahashi, T.Fujishiro, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.54 / 1.50
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	107.021, 107.021, 65.753, 90, 90, 120
R / Rfree (%)	17.7 / 19.7

The binding sites of Iron atom in the Crystal Structure of Iscu H106A Variant (pdb code 7c8n). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Iscu H106A Variant, PDB code: 7c8n:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of Iscu H106A Variant


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Iscu H106A Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:17.4 occ:1.00 FE1 A:FES201 0.0 17.4 1.0 S2 A:FES201 2.2 18.6 1.0 S1 A:FES201 2.3 17.4 1.0 SG A:CYS107 2.3 19.1 1.0 SG A:CYS63 2.3 17.5 1.0 FE2 A:FES201 2.8 18.0 1.0 CB A:CYS107 3.2 18.5 1.0 CB A:CYS63 3.3 16.6 1.0 NZ A:LYS104 3.9 21.2 1.0 N A:CYS63 3.9 16.9 1.0 ND2 A:ASN35 4.1 29.1 1.0 CA A:CYS63 4.2 16.4 1.0 CA A:CYS107 4.3 19.4 1.0 O A:HOH386 4.3 37.4 1.0 SG A:CYS38 4.4 19.3 1.0 O A:HOH335 4.5 23.9 1.0 CB A:ALA66 4.5 18.2 1.0 CD A:LYS104 4.5 19.4 1.0 OD2 A:ASP40 4.6 18.2 1.0 CE A:LYS104 4.9 19.1 1.0 N A:ALA66 5.0 17.3 1.0

Iron binding site 2 out of 2 in the Crystal Structure of Iscu H106A Variant


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Iscu H106A Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe201 b:18.0 occ:1.00 FE2 A:FES201 0.0 18.0 1.0 OD2 A:ASP40 2.1 18.2 1.0 S1 A:FES201 2.2 17.4 1.0 SG A:CYS38 2.3 19.3 1.0 S2 A:FES201 2.3 18.6 1.0 FE1 A:FES201 2.8 17.4 1.0 CG A:ASP40 3.0 18.7 1.0 CB A:CYS38 3.2 18.4 1.0 CB A:ASP40 3.4 16.9 1.0 OD1 A:ASP40 4.1 18.4 1.0 ND2 A:ASN35 4.1 29.1 1.0 N A:CYS63 4.2 16.9 1.0 CA A:GLY62 4.2 17.4 1.0 O A:HOH386 4.5 37.4 1.0 N A:ASP40 4.5 17.2 1.0 SG A:CYS107 4.6 19.1 1.0 SG A:CYS63 4.6 17.5 1.0 CA A:ASP40 4.6 16.5 1.0 CB A:ASN35 4.6 22.3 1.0 CB A:CYS63 4.6 16.6 1.0 CG A:ASN35 4.7 27.3 1.0 CA A:CYS38 4.7 20.4 1.0 C A:GLY62 4.8 16.3 1.0

K.Kunichika, R.Nakamura, T.Fujishiro, Y.Takahashi. The Structure of the Dimeric State of Iscu Harboring Two Adjacent [2FE-2S] Clusters Provides Mechanistic Insights Into Cluster Conversion to [4FE-4S]. Biochemistry 2021.
ISSN: ISSN 0006-2960
PubMed: 33938220
DOI: 10.1021/ACS.BIOCHEM.1C00112