Iron in PDB 7eqh: Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme

All present enzymatic activity of Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme:
1.14.14.18;

The structure of Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme, PDB code: 7eqh was solved by X.Li, J.Wang, L.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.75 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	66.622, 84.759, 92.87, 90, 90, 90
R / Rfree (%)	20.8 / 25

The binding sites of Iron atom in the Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme (pdb code 7eqh). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 2 binding sites of Iron where determined in the Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme, PDB code: 7eqh:
Jump to Iron binding site number: 1; 2;

Iron binding site 1 out of 2 in the Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe300 b:23.8 occ:1.00 FE A:HEM300 0.0 23.8 1.0 NB A:HEM300 2.0 30.7 1.0 NA A:HEM300 2.0 28.8 1.0 NC A:HEM300 2.1 27.0 1.0 ND A:HEM300 2.1 26.0 1.0 NE2 A:HIS86 2.3 25.0 1.0 O A:HOH433 2.5 31.3 1.0 C1B A:HEM300 3.0 29.2 1.0 C4A A:HEM300 3.0 29.5 1.0 CD2 A:HIS86 3.0 28.1 1.0 C4B A:HEM300 3.0 31.7 1.0 C4C A:HEM300 3.1 25.9 1.0 C1D A:HEM300 3.1 25.0 1.0 C1A A:HEM300 3.1 29.7 1.0 C1C A:HEM300 3.1 28.2 1.0 C4D A:HEM300 3.1 26.0 1.0 CHB A:HEM300 3.4 28.4 1.0 CE1 A:HIS86 3.4 26.5 1.0 CHD A:HEM300 3.4 21.7 1.0 CHC A:HEM300 3.4 29.4 1.0 CHA A:HEM300 3.5 26.8 1.0 C2B A:HEM300 4.2 33.5 1.0 O A:HOH473 4.2 31.3 1.0 C3B A:HEM300 4.2 30.4 1.0 C3A A:HEM300 4.3 31.4 1.0 CG A:HIS86 4.3 31.4 1.0 C2A A:HEM300 4.3 33.7 1.0 C2D A:HEM300 4.3 23.1 1.0 C3C A:HEM300 4.3 29.5 1.0 C2C A:HEM300 4.3 25.8 1.0 C3D A:HEM300 4.3 25.2 1.0 N A:GLY209 4.3 28.5 1.0 ND1 A:HIS86 4.4 28.7 1.0 CA A:GLY209 4.6 29.2 1.0 CA A:ALA205 4.8 25.2 1.0 CB A:ALA205 4.8 22.8 1.0 CB A:ALA208 4.9 28.6 1.0

Iron binding site 2 out of 2 in the Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Crystal Structure of Arabidopsis GUN2/HO1 in Complex with Heme within 5.0Å range: probe atom residue distance (Å) B Occ B:Fe300 b:24.6 occ:1.00 FE B:HEM300 0.0 24.6 1.0 NB B:HEM300 2.0 29.6 1.0 NA B:HEM300 2.0 31.4 1.0 NC B:HEM300 2.1 27.6 1.0 ND B:HEM300 2.1 25.1 1.0 NE2 B:HIS86 2.3 28.1 1.0 O B:HOH421 2.5 32.6 1.0 C1B B:HEM300 3.0 29.4 1.0 C4A B:HEM300 3.0 29.8 1.0 C4B B:HEM300 3.1 33.3 1.0 C4C B:HEM300 3.1 28.4 1.0 C1D B:HEM300 3.1 26.8 1.0 C1C B:HEM300 3.1 28.6 1.0 C1A B:HEM300 3.1 30.8 1.0 C4D B:HEM300 3.1 25.5 1.0 CD2 B:HIS86 3.1 29.4 1.0 CHB B:HEM300 3.4 27.5 1.0 CE1 B:HIS86 3.4 28.0 1.0 CHD B:HEM300 3.4 24.6 1.0 CHC B:HEM300 3.4 30.5 1.0 CHA B:HEM300 3.5 27.6 1.0 C2B B:HEM300 4.3 33.9 1.0 C3B B:HEM300 4.3 32.4 1.0 C3A B:HEM300 4.3 34.3 1.0 C2A B:HEM300 4.3 31.6 1.0 C3C B:HEM300 4.3 27.4 1.0 C2D B:HEM300 4.3 25.5 1.0 C2C B:HEM300 4.3 29.7 1.0 C3D B:HEM300 4.3 27.5 1.0 CG B:HIS86 4.4 30.6 1.0 N B:GLY209 4.4 28.8 1.0 O B:HOH472 4.4 33.2 1.0 ND1 B:HIS86 4.5 28.9 1.0 CA B:GLY209 4.7 32.3 1.0 CA B:ALA205 4.8 25.9 1.0 CB B:ALA205 4.8 22.8 1.0 CB B:ALA208 4.9 26.7 1.0

J.Wang, X.Li, J.W.Chang, T.Ye, Y.Mao, X.Wang, L.Liu. Enzymological and Structural Characterization of Arabidopsis Thaliana Heme Oxygenase-1. Febs Open Bio V. 12 1677 2022.
ISSN: ESSN 2211-5463
PubMed: 35689519
DOI: 10.1002/2211-5463.13453