Iron in PDB 7ev7: Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
Enzymatic activity of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
All present enzymatic activity of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K:
7.1.1.9;
Protein crystallography data
The structure of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K, PDB code: 7ev7
was solved by
A.Shimada,
S.Yoshikawa,
T.Tsukihara,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
39.74 /
1.70
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
181.539,
203.655,
177.569,
90,
90,
90
|
R / Rfree (%)
|
15.9 /
17.8
|
Other elements in 7ev7:
The structure of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K also contains other interesting chemical elements:
Iron Binding Sites:
The binding sites of Iron atom in the Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
(pdb code 7ev7). This binding sites where shown within
5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the
Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K, PDB code: 7ev7:
Jump to Iron binding site number:
1;
2;
3;
4;
Iron binding site 1 out
of 4 in 7ev7
Go back to
Iron Binding Sites List in 7ev7
Iron binding site 1 out
of 4 in the Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 1 of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe602
b:21.4
occ:1.00
|
FE
|
A:HEA602
|
0.0
|
21.4
|
1.0
|
NE2
|
A:HIS378
|
2.0
|
19.9
|
1.0
|
NB
|
A:HEA602
|
2.0
|
21.4
|
1.0
|
ND
|
A:HEA602
|
2.0
|
20.6
|
1.0
|
NA
|
A:HEA602
|
2.0
|
20.1
|
1.0
|
NE2
|
A:HIS61
|
2.0
|
21.5
|
1.0
|
NC
|
A:HEA602
|
2.0
|
20.3
|
1.0
|
CE1
|
A:HIS378
|
2.9
|
21.7
|
1.0
|
C4A
|
A:HEA602
|
3.0
|
19.3
|
1.0
|
CE1
|
A:HIS61
|
3.0
|
23.4
|
1.0
|
CD2
|
A:HIS61
|
3.0
|
21.3
|
1.0
|
C4C
|
A:HEA602
|
3.0
|
20.8
|
1.0
|
C4D
|
A:HEA602
|
3.0
|
20.4
|
1.0
|
C1D
|
A:HEA602
|
3.0
|
19.4
|
1.0
|
C1B
|
A:HEA602
|
3.0
|
19.2
|
1.0
|
C1A
|
A:HEA602
|
3.0
|
22.0
|
1.0
|
C4B
|
A:HEA602
|
3.1
|
20.2
|
1.0
|
C1C
|
A:HEA602
|
3.1
|
23.4
|
1.0
|
CD2
|
A:HIS378
|
3.1
|
23.1
|
1.0
|
CHB
|
A:HEA602
|
3.4
|
21.1
|
1.0
|
CHC
|
A:HEA602
|
3.5
|
20.3
|
1.0
|
CHD
|
A:HEA602
|
3.5
|
20.6
|
1.0
|
CHA
|
A:HEA602
|
3.5
|
22.1
|
1.0
|
ND1
|
A:HIS378
|
4.1
|
21.0
|
1.0
|
ND1
|
A:HIS61
|
4.1
|
22.1
|
1.0
|
CG
|
A:HIS61
|
4.2
|
21.3
|
1.0
|
CG
|
A:HIS378
|
4.2
|
20.4
|
1.0
|
C3A
|
A:HEA602
|
4.2
|
19.4
|
1.0
|
C2A
|
A:HEA602
|
4.2
|
19.8
|
1.0
|
C2C
|
A:HEA602
|
4.2
|
20.0
|
1.0
|
C2D
|
A:HEA602
|
4.2
|
19.0
|
1.0
|
C3D
|
A:HEA602
|
4.2
|
18.8
|
1.0
|
C3C
|
A:HEA602
|
4.3
|
19.3
|
1.0
|
C3B
|
A:HEA602
|
4.3
|
19.4
|
1.0
|
C2B
|
A:HEA602
|
4.3
|
20.9
|
1.0
|
OG
|
A:SER382
|
4.7
|
25.3
|
0.3
|
CE1
|
A:PHE377
|
4.9
|
21.3
|
1.0
|
|
Iron binding site 2 out
of 4 in 7ev7
Go back to
Iron Binding Sites List in 7ev7
Iron binding site 2 out
of 4 in the Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 2 of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Fe603
b:20.9
occ:1.00
|
FE
|
A:HEA603
|
0.0
|
20.9
|
1.0
|
C
|
A:CMO607
|
1.8
|
16.2
|
0.1
|
ND
|
A:HEA603
|
2.0
|
20.3
|
1.0
|
NB
|
A:HEA603
|
2.0
|
21.1
|
1.0
|
NA
|
A:HEA603
|
2.1
|
20.1
|
1.0
|
NC
|
A:HEA603
|
2.1
|
19.9
|
1.0
|
NE2
|
A:HIS376
|
2.1
|
23.1
|
1.0
|
O
|
A:CMO607
|
3.0
|
16.2
|
0.1
|
C4B
|
A:HEA603
|
3.0
|
23.0
|
1.0
|
C4A
|
A:HEA603
|
3.0
|
19.0
|
1.0
|
C4D
|
A:HEA603
|
3.0
|
21.0
|
1.0
|
C1A
|
A:HEA603
|
3.1
|
20.4
|
1.0
|
CE1
|
A:HIS376
|
3.1
|
23.4
|
1.0
|
C1D
|
A:HEA603
|
3.1
|
20.1
|
1.0
|
C4C
|
A:HEA603
|
3.1
|
20.0
|
1.0
|
C1C
|
A:HEA603
|
3.1
|
22.5
|
1.0
|
CD2
|
A:HIS376
|
3.1
|
20.0
|
1.0
|
C1B
|
A:HEA603
|
3.1
|
19.6
|
1.0
|
C
|
A:CMO607
|
3.5
|
16.2
|
0.6
|
CHA
|
A:HEA603
|
3.5
|
19.2
|
1.0
|
CHB
|
A:HEA603
|
3.5
|
20.4
|
1.0
|
CHC
|
A:HEA603
|
3.5
|
22.9
|
1.0
|
CHD
|
A:HEA603
|
3.5
|
18.7
|
1.0
|
O
|
A:CMO607
|
3.6
|
17.0
|
0.6
|
ND1
|
A:HIS376
|
4.2
|
22.4
|
1.0
|
C3A
|
A:HEA603
|
4.2
|
20.8
|
1.0
|
C3D
|
A:HEA603
|
4.3
|
20.0
|
1.0
|
C3B
|
A:HEA603
|
4.3
|
20.5
|
1.0
|
CG
|
A:HIS376
|
4.3
|
20.7
|
1.0
|
C2A
|
A:HEA603
|
4.3
|
19.8
|
1.0
|
C2D
|
A:HEA603
|
4.3
|
20.8
|
1.0
|
C2C
|
A:HEA603
|
4.3
|
19.3
|
1.0
|
C3C
|
A:HEA603
|
4.3
|
19.1
|
1.0
|
C2B
|
A:HEA603
|
4.3
|
21.8
|
1.0
|
CG1
|
A:VAL380
|
4.6
|
19.5
|
0.3
|
CU
|
A:CU604
|
4.9
|
20.9
|
1.0
|
CA
|
A:GLY355
|
5.0
|
22.3
|
1.0
|
|
Iron binding site 3 out
of 4 in 7ev7
Go back to
Iron Binding Sites List in 7ev7
Iron binding site 3 out
of 4 in the Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 3 of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Fe602
b:24.8
occ:1.00
|
FE
|
N:HEA602
|
0.0
|
24.8
|
1.0
|
NB
|
N:HEA602
|
2.0
|
26.3
|
1.0
|
NE2
|
N:HIS378
|
2.0
|
25.3
|
1.0
|
ND
|
N:HEA602
|
2.0
|
25.6
|
1.0
|
NC
|
N:HEA602
|
2.0
|
23.3
|
1.0
|
NE2
|
N:HIS61
|
2.0
|
23.2
|
1.0
|
NA
|
N:HEA602
|
2.0
|
22.9
|
1.0
|
CE1
|
N:HIS378
|
2.9
|
27.9
|
1.0
|
C4A
|
N:HEA602
|
3.0
|
25.4
|
1.0
|
C1C
|
N:HEA602
|
3.0
|
25.6
|
1.0
|
CD2
|
N:HIS61
|
3.0
|
23.8
|
1.0
|
C4B
|
N:HEA602
|
3.0
|
24.6
|
1.0
|
CE1
|
N:HIS61
|
3.0
|
25.5
|
1.0
|
C4C
|
N:HEA602
|
3.0
|
23.1
|
1.0
|
C1A
|
N:HEA602
|
3.0
|
26.3
|
1.0
|
C1D
|
N:HEA602
|
3.0
|
21.7
|
1.0
|
CD2
|
N:HIS378
|
3.1
|
23.2
|
1.0
|
C1B
|
N:HEA602
|
3.1
|
24.1
|
1.0
|
C4D
|
N:HEA602
|
3.1
|
25.1
|
1.0
|
CHC
|
N:HEA602
|
3.4
|
25.6
|
1.0
|
CHD
|
N:HEA602
|
3.4
|
23.1
|
1.0
|
CHB
|
N:HEA602
|
3.4
|
25.6
|
1.0
|
CHA
|
N:HEA602
|
3.5
|
26.5
|
1.0
|
ND1
|
N:HIS378
|
4.1
|
25.4
|
1.0
|
ND1
|
N:HIS61
|
4.1
|
24.9
|
1.0
|
CG
|
N:HIS378
|
4.2
|
24.8
|
1.0
|
CG
|
N:HIS61
|
4.2
|
25.1
|
1.0
|
C3A
|
N:HEA602
|
4.2
|
22.2
|
1.0
|
C2A
|
N:HEA602
|
4.2
|
22.9
|
1.0
|
C2C
|
N:HEA602
|
4.2
|
21.9
|
1.0
|
C3B
|
N:HEA602
|
4.2
|
23.9
|
1.0
|
C3C
|
N:HEA602
|
4.3
|
22.0
|
1.0
|
C2D
|
N:HEA602
|
4.3
|
22.8
|
1.0
|
C2B
|
N:HEA602
|
4.3
|
25.6
|
1.0
|
C3D
|
N:HEA602
|
4.3
|
24.8
|
1.0
|
OG
|
N:SER382
|
4.7
|
28.2
|
0.3
|
CE1
|
N:PHE377
|
4.9
|
24.4
|
1.0
|
|
Iron binding site 4 out
of 4 in 7ev7
Go back to
Iron Binding Sites List in 7ev7
Iron binding site 4 out
of 4 in the Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K
Mono view
Stereo pair view
|
A full contact list of Iron with other atoms in the Fe binding
site number 4 of Bovine Heart Cytochrome C Oxidase in the Carbon Monoxide-Bound Fully Reduced State at A 50 K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Fe603
b:23.4
occ:1.00
|
FE
|
N:HEA603
|
0.0
|
23.4
|
1.0
|
C
|
N:CMO607
|
1.8
|
19.4
|
0.2
|
ND
|
N:HEA603
|
2.0
|
21.6
|
1.0
|
NB
|
N:HEA603
|
2.0
|
23.2
|
1.0
|
NC
|
N:HEA603
|
2.0
|
21.9
|
1.0
|
NA
|
N:HEA603
|
2.1
|
23.2
|
1.0
|
NE2
|
N:HIS376
|
2.1
|
25.2
|
1.0
|
O
|
N:CMO607
|
2.9
|
18.0
|
0.2
|
C4D
|
N:HEA603
|
3.0
|
24.0
|
1.0
|
C1A
|
N:HEA603
|
3.0
|
24.3
|
1.0
|
C4C
|
N:HEA603
|
3.1
|
22.5
|
1.0
|
C4A
|
N:HEA603
|
3.1
|
20.8
|
1.0
|
C4B
|
N:HEA603
|
3.1
|
21.7
|
1.0
|
C1C
|
N:HEA603
|
3.1
|
24.5
|
1.0
|
C1B
|
N:HEA603
|
3.1
|
22.4
|
1.0
|
C1D
|
N:HEA603
|
3.1
|
20.8
|
1.0
|
CE1
|
N:HIS376
|
3.1
|
23.6
|
1.0
|
CD2
|
N:HIS376
|
3.1
|
24.4
|
1.0
|
CHA
|
N:HEA603
|
3.4
|
21.7
|
1.0
|
C
|
N:CMO607
|
3.4
|
19.6
|
0.6
|
CHB
|
N:HEA603
|
3.5
|
23.1
|
1.0
|
CHC
|
N:HEA603
|
3.5
|
24.9
|
1.0
|
CHD
|
N:HEA603
|
3.5
|
22.1
|
1.0
|
O
|
N:CMO607
|
3.7
|
19.7
|
0.6
|
ND1
|
N:HIS376
|
4.2
|
24.8
|
1.0
|
C3D
|
N:HEA603
|
4.3
|
24.2
|
1.0
|
C2A
|
N:HEA603
|
4.3
|
22.8
|
1.0
|
C3A
|
N:HEA603
|
4.3
|
23.4
|
1.0
|
C2C
|
N:HEA603
|
4.3
|
23.0
|
1.0
|
CG
|
N:HIS376
|
4.3
|
23.5
|
1.0
|
C3B
|
N:HEA603
|
4.3
|
21.3
|
1.0
|
C2D
|
N:HEA603
|
4.3
|
22.7
|
1.0
|
C3C
|
N:HEA603
|
4.3
|
22.4
|
1.0
|
C2B
|
N:HEA603
|
4.3
|
23.6
|
1.0
|
CG1
|
N:VAL380
|
4.6
|
21.4
|
0.3
|
CU
|
N:CU604
|
4.9
|
23.6
|
1.0
|
CA
|
N:GLY355
|
5.0
|
26.0
|
1.0
|
|
Reference:
A.Shimada,
K.Shinzawa-Itoh,
K.Muramoto,
T.Tsukihara,
S.Yoshikawa.
Structure of Co-Bound Fully Reduced State of Bovine Heart Cytochrome C Oxidase To Be Published.
Page generated: Thu Aug 8 05:27:19 2024
|