Iron in PDB 7lw7: Human Exonuclease 5 Crystal Structure

The structure of Human Exonuclease 5 Crystal Structure, PDB code: 7lw7 was solved by C.L.Tsai, J.A.Tainer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.98 / 2.50
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.818, 83.836, 95.965, 90, 90, 90
R / Rfree (%)	18.2 / 22.5

The binding sites of Iron atom in the Human Exonuclease 5 Crystal Structure (pdb code 7lw7). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 4 binding sites of Iron where determined in the Human Exonuclease 5 Crystal Structure, PDB code: 7lw7:
Jump to Iron binding site number: 1; 2; 3; 4;

Iron binding site 1 out of 4 in the Human Exonuclease 5 Crystal Structure


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Human Exonuclease 5 Crystal Structure within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe502 b:26.5 occ:1.00 FE1 A:SF4502 0.0 26.5 1.0 SG A:CYS346 2.3 27.4 1.0 S2 A:SF4502 2.3 26.6 1.0 S3 A:SF4502 2.3 27.7 1.0 S4 A:SF4502 2.3 23.6 1.0 FE3 A:SF4502 2.7 27.4 1.0 FE4 A:SF4502 2.7 30.2 1.0 FE2 A:SF4502 2.7 26.8 1.0 CB A:CYS346 3.1 29.1 1.0 S1 A:SF4502 3.9 24.7 1.0 NE2 A:GLN95 4.0 25.0 1.0 CB A:GLN95 4.3 30.4 1.0 CA A:CYS346 4.6 33.0 1.0 CA A:CYS343 4.7 30.5 1.0 C A:TYR348 4.7 33.6 1.0 CG A:GLN95 4.7 28.4 1.0 SG A:CYS92 4.8 24.8 1.0 SG A:CYS343 4.8 33.0 1.0 N A:ALA349 4.8 33.3 1.0 SG A:CYS352 4.8 36.4 1.0 CB A:CYS343 4.8 23.6 1.0 O A:TYR348 4.8 41.4 1.0 CD A:GLN95 4.8 35.2 1.0 CA A:GLN95 4.9 28.2 1.0 CA A:ALA349 4.9 31.0 1.0 CB A:CYS352 4.9 33.1 1.0

Iron binding site 2 out of 4 in the Human Exonuclease 5 Crystal Structure


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Human Exonuclease 5 Crystal Structure within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe502 b:26.8 occ:1.00 FE2 A:SF4502 0.0 26.8 1.0 SG A:CYS343 2.3 33.0 1.0 S1 A:SF4502 2.3 24.7 1.0 S3 A:SF4502 2.3 27.7 1.0 S4 A:SF4502 2.3 23.6 1.0 FE4 A:SF4502 2.7 30.2 1.0 FE3 A:SF4502 2.7 27.4 1.0 FE1 A:SF4502 2.7 26.5 1.0 CB A:CYS343 3.2 23.6 1.0 CA A:CYS343 3.7 30.5 1.0 S2 A:SF4502 3.9 26.6 1.0 N A:CYS343 4.1 21.9 1.0 CG2 A:VAL335 4.4 17.3 1.0 CG1 A:VAL335 4.4 21.2 1.0 CB A:CYS346 4.7 29.1 1.0 SG A:CYS346 4.7 27.4 1.0 CG A:ARG355 4.7 69.0 1.0 SG A:CYS92 4.8 24.8 1.0 SG A:CYS352 4.8 36.4 1.0 C A:LYS342 4.8 28.4 1.0

Iron binding site 3 out of 4 in the Human Exonuclease 5 Crystal Structure


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Human Exonuclease 5 Crystal Structure within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe502 b:27.4 occ:1.00 FE3 A:SF4502 0.0 27.4 1.0 S4 A:SF4502 2.3 23.6 1.0 S2 A:SF4502 2.3 26.6 1.0 S1 A:SF4502 2.3 24.7 1.0 SG A:CYS92 2.3 24.8 1.0 FE1 A:SF4502 2.7 26.5 1.0 FE2 A:SF4502 2.7 26.8 1.0 FE4 A:SF4502 2.7 30.2 1.0 CB A:CYS92 3.5 25.7 1.0 S3 A:SF4502 3.9 27.7 1.0 CG1 A:VAL335 4.0 21.2 1.0 N A:GLN95 4.0 30.5 1.0 CB A:GLN95 4.1 30.4 1.0 CB A:LEU94 4.2 24.3 1.0 CA A:GLN95 4.5 28.2 1.0 N A:VAL335 4.5 29.5 1.0 CA A:GLY334 4.7 22.0 1.0 C A:LEU94 4.7 31.3 1.0 SG A:CYS352 4.7 36.4 1.0 SG A:CYS346 4.7 27.4 1.0 SG A:CYS343 4.8 33.0 1.0 CA A:LEU94 4.9 20.0 1.0 NE2 A:GLN95 4.9 25.0 1.0 CA A:CYS92 4.9 27.0 1.0

Iron binding site 4 out of 4 in the Human Exonuclease 5 Crystal Structure


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Human Exonuclease 5 Crystal Structure within 5.0Å range: probe atom residue distance (Å) B Occ A:Fe502 b:30.2 occ:1.00 FE4 A:SF4502 0.0 30.2 1.0 SG A:CYS352 2.3 36.4 1.0 S1 A:SF4502 2.3 24.7 1.0 S3 A:SF4502 2.3 27.7 1.0 S2 A:SF4502 2.3 26.6 1.0 FE2 A:SF4502 2.7 26.8 1.0 FE1 A:SF4502 2.7 26.5 1.0 FE3 A:SF4502 2.7 27.4 1.0 CB A:CYS352 3.1 33.1 1.0 S4 A:SF4502 3.9 23.6 1.0 CA A:CYS352 4.6 39.9 1.0 CB A:ARG355 4.6 67.4 1.0 N A:ARG355 4.6 67.1 1.0 CD2 A:LEU94 4.6 27.2 1.0 SG A:CYS343 4.7 33.0 1.0 CB A:LEU94 4.7 24.3 1.0 CB A:TRP354 4.8 22.7 1.0 SG A:CYS346 4.8 27.4 1.0 SG A:CYS92 4.8 24.8 1.0 CG A:LEU94 4.9 37.5 1.0 O A:TYR348 4.9 41.4 1.0 CE2 A:TYR98 4.9 33.1 1.0

S.Hambarde, C.L.Tsai, R.K.Pandita, A.Bacolla, A.Maitra, V.Charaka, C.R.Hunt, R.Kumar, O.Limbo, R.Le Meur, W.J.Chazin, S.E.Tsutakawa, P.Russell, K.Schlacher, T.K.Pandita, J.A.Tainer. EXO5-Dna Structure and Blm Interactions Direct Dna Resection Critical For Atr-Dependent Replication Restart. Mol.Cell 2021.
ISSN: ISSN 1097-2765
PubMed: 34197737
DOI: 10.1016/J.MOLCEL.2021.05.027