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Iron in PDB 7mi4: Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex

Enzymatic activity of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex

All present enzymatic activity of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex:
3.1.12.1;

Other elements in 7mi4:

The structure of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex also contains other interesting chemical elements:

Manganese (Mn) 8 atoms

Iron Binding Sites:

The binding sites of Iron atom in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex (pdb code 7mi4). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 8 binding sites of Iron where determined in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex, PDB code: 7mi4:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Iron binding site 1 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 1 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe601

b:56.5
occ:1.00
 FE1 A:SF4601 0.0 56.5 1.0
S3 A:SF4601 2.3 56.5 1.0
S2 A:SF4601 2.3 56.5 1.0
S4 A:SF4601 2.3 56.5 1.0
SG A:CYS22 2.3 50.2 1.0
FE4 A:SF4601 2.7 56.5 1.0
FE3 A:SF4601 2.7 56.5 1.0
FE2 A:SF4601 2.7 56.5 1.0
CB A:CYS22 3.3 50.2 1.0
CB A:LEU25 3.5 45.5 1.0
N A:LEU25 3.6 45.5 1.0
S1 A:SF4601 3.9 56.5 1.0
CA A:LEU25 3.9 45.5 1.0
SG A:CYS196 4.2 54.7 1.0
SG A:CYS190 4.4 55.0 1.0
CG A:LEU25 4.4 45.5 1.0
CB A:ARG24 4.5 49.2 1.0
C A:ARG24 4.5 49.2 1.0
CD1 A:LEU25 4.5 45.5 1.0
CA A:CYS22 4.7 50.2 1.0
CD2 A:LEU25 4.7 45.5 1.0
O A:CYS22 4.7 50.2 1.0
SG A:CYS187 4.7 56.9 1.0
CB A:CYS190 4.8 55.0 1.0
CA A:ARG24 4.9 49.2 1.0
C A:CYS22 4.9 50.2 1.0
CB A:CYS196 5.0 54.7 1.0

Iron binding site 2 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 2 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe601

b:56.5
occ:1.00
 FE2 A:SF4601 0.0 56.5 1.0
S4 A:SF4601 2.3 56.5 1.0
SG A:CYS196 2.3 54.7 1.0
S1 A:SF4601 2.3 56.5 1.0
S3 A:SF4601 2.3 56.5 1.0
FE3 A:SF4601 2.7 56.5 1.0
FE1 A:SF4601 2.7 56.5 1.0
FE4 A:SF4601 2.7 56.5 1.0
CB A:CYS196 3.3 54.7 1.0
SG A:CYS187 3.6 56.9 1.0
S2 A:SF4601 3.9 56.5 1.0
SG A:CYS22 4.0 50.2 1.0
CB A:PRO198 4.2 60.7 1.0
CB A:PRO180 4.3 53.1 1.0
CD A:ARG24 4.4 49.2 1.0
NE A:ARG24 4.4 49.2 1.0
CA A:PRO180 4.5 53.1 1.0
N A:LEU181 4.7 57.8 1.0
CB A:ARG24 4.8 49.2 1.0
CA A:CYS196 4.8 54.7 1.0
CG A:PRO198 4.8 60.7 1.0
CG A:ARG24 4.8 49.2 1.0
CB A:CYS187 4.9 56.9 1.0
SG A:CYS190 4.9 55.0 1.0
CA A:PRO198 4.9 60.7 1.0

Iron binding site 3 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 3 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe601

b:56.5
occ:1.00
 FE3 A:SF4601 0.0 56.5 1.0
SG A:CYS190 2.2 55.0 1.0
S4 A:SF4601 2.3 56.5 1.0
S1 A:SF4601 2.3 56.5 1.0
S2 A:SF4601 2.3 56.5 1.0
FE2 A:SF4601 2.7 56.5 1.0
FE4 A:SF4601 2.7 56.5 1.0
FE1 A:SF4601 2.7 56.5 1.0
CB A:CYS190 3.2 55.0 1.0
SG A:CYS187 3.8 56.9 1.0
S3 A:SF4601 3.9 56.5 1.0
CB A:CYS196 4.0 54.7 1.0
SG A:CYS196 4.1 54.7 1.0
CA A:CYS187 4.1 56.9 1.0
CB A:CYS187 4.2 56.9 1.0
O A:CYS187 4.4 56.9 1.0
N A:VAL193 4.5 52.7 1.0
CA A:VAL193 4.6 52.7 1.0
CA A:CYS190 4.6 55.0 1.0
SG A:CYS22 4.8 50.2 1.0
C A:CYS187 4.8 56.9 1.0
C A:LEU192 4.8 48.8 1.0
CB A:VAL193 4.9 52.7 1.0

Iron binding site 4 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 4 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe601

b:56.5
occ:1.00
 FE4 A:SF4601 0.0 56.5 1.0
SG A:CYS187 2.1 56.9 1.0
S2 A:SF4601 2.3 56.5 1.0
S1 A:SF4601 2.3 56.5 1.0
S3 A:SF4601 2.3 56.5 1.0
FE3 A:SF4601 2.7 56.5 1.0
FE1 A:SF4601 2.7 56.5 1.0
FE2 A:SF4601 2.7 56.5 1.0
SG A:CYS22 3.4 50.2 1.0
CB A:CYS187 3.5 56.9 1.0
CA A:CYS187 3.7 56.9 1.0
S4 A:SF4601 3.9 56.5 1.0
CG A:LEU181 3.9 57.8 1.0
N A:CYS187 4.1 56.9 1.0
CB A:LEU181 4.2 57.8 1.0
CB A:CYS22 4.3 50.2 1.0
CD1 A:LEU181 4.3 57.8 1.0
SG A:CYS190 4.6 55.0 1.0
N A:LEU181 4.7 57.8 1.0
CB A:CYS190 4.8 55.0 1.0
C A:LYS186 4.9 56.9 1.0
CB A:LYS186 4.9 56.9 1.0
SG A:CYS196 5.0 54.7 1.0

Iron binding site 5 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 5 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe601

b:57.2
occ:1.00
 FE1 C:SF4601 0.0 57.2 1.0
S3 C:SF4601 2.3 57.2 1.0
S2 C:SF4601 2.3 57.2 1.0
S4 C:SF4601 2.3 57.2 1.0
SG C:CYS22 2.3 52.3 1.0
FE3 C:SF4601 2.7 57.2 1.0
FE4 C:SF4601 2.7 57.2 1.0
FE2 C:SF4601 2.7 57.2 1.0
CB C:CYS22 3.3 52.3 1.0
CB C:LEU25 3.4 46.7 1.0
N C:LEU25 3.6 46.7 1.0
S1 C:SF4601 3.9 57.2 1.0
CA C:LEU25 3.9 46.7 1.0
SG C:CYS196 4.2 53.2 1.0
SG C:CYS190 4.4 55.1 1.0
CG C:LEU25 4.4 46.7 1.0
CB C:ARG24 4.5 50.0 1.0
C C:ARG24 4.5 50.0 1.0
CD1 C:LEU25 4.5 46.7 1.0
CA C:CYS22 4.7 52.3 1.0
CD2 C:LEU25 4.7 46.7 1.0
O C:CYS22 4.7 52.3 1.0
SG C:CYS187 4.7 57.7 1.0
CB C:CYS190 4.8 55.1 1.0
CA C:ARG24 4.9 50.0 1.0
C C:CYS22 4.9 52.3 1.0
CB C:CYS196 5.0 53.2 1.0

Iron binding site 6 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 6 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe601

b:57.2
occ:1.00
 FE2 C:SF4601 0.0 57.2 1.0
S4 C:SF4601 2.3 57.2 1.0
SG C:CYS196 2.3 53.2 1.0
S1 C:SF4601 2.3 57.2 1.0
S3 C:SF4601 2.3 57.2 1.0
FE3 C:SF4601 2.7 57.2 1.0
FE1 C:SF4601 2.7 57.2 1.0
FE4 C:SF4601 2.7 57.2 1.0
CB C:CYS196 3.3 53.2 1.0
SG C:CYS187 3.6 57.7 1.0
S2 C:SF4601 3.9 57.2 1.0
SG C:CYS22 4.0 52.3 1.0
CB C:PRO198 4.2 60.5 1.0
CB C:PRO180 4.3 54.6 1.0
CD C:ARG24 4.4 50.0 1.0
NE C:ARG24 4.4 50.0 1.0
CA C:PRO180 4.5 54.6 1.0
N C:LEU181 4.7 60.8 1.0
CB C:ARG24 4.8 50.0 1.0
CA C:CYS196 4.8 53.2 1.0
CG C:PRO198 4.8 60.5 1.0
CG C:ARG24 4.8 50.0 1.0
CB C:CYS187 4.9 57.7 1.0
SG C:CYS190 4.9 55.1 1.0
CA C:PRO198 4.9 60.5 1.0

Iron binding site 7 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 7 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe601

b:57.2
occ:1.00
 FE3 C:SF4601 0.0 57.2 1.0
SG C:CYS190 2.2 55.1 1.0
S4 C:SF4601 2.3 57.2 1.0
S1 C:SF4601 2.3 57.2 1.0
S2 C:SF4601 2.3 57.2 1.0
FE2 C:SF4601 2.7 57.2 1.0
FE4 C:SF4601 2.7 57.2 1.0
FE1 C:SF4601 2.7 57.2 1.0
CB C:CYS190 3.2 55.1 1.0
SG C:CYS187 3.8 57.7 1.0
S3 C:SF4601 3.9 57.2 1.0
CB C:CYS196 4.0 53.2 1.0
SG C:CYS196 4.1 53.2 1.0
CA C:CYS187 4.1 57.7 1.0
CB C:CYS187 4.2 57.7 1.0
O C:CYS187 4.4 57.7 1.0
N C:VAL193 4.5 52.7 1.0
CA C:VAL193 4.6 52.7 1.0
CA C:CYS190 4.6 55.1 1.0
SG C:CYS22 4.8 52.3 1.0
C C:CYS187 4.8 57.7 1.0
C C:LEU192 4.8 49.8 1.0
CB C:VAL193 4.9 52.7 1.0

Iron binding site 8 out of 8 in 7mi4

Go back to Iron Binding Sites List in 7mi4
Iron binding site 8 out of 8 in the Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 8 of Symmetrical Pam-Pam Prespacer Bound CAS4/CAS1/CAS2 Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Fe601

b:57.2
occ:1.00
 FE4 C:SF4601 0.0 57.2 1.0
SG C:CYS187 2.1 57.7 1.0
S2 C:SF4601 2.3 57.2 1.0
S1 C:SF4601 2.3 57.2 1.0
S3 C:SF4601 2.3 57.2 1.0
FE3 C:SF4601 2.7 57.2 1.0
FE1 C:SF4601 2.7 57.2 1.0
FE2 C:SF4601 2.7 57.2 1.0
SG C:CYS22 3.4 52.3 1.0
CB C:CYS187 3.5 57.7 1.0
CA C:CYS187 3.7 57.7 1.0
S4 C:SF4601 3.9 57.2 1.0
CG C:LEU181 3.9 60.8 1.0
N C:CYS187 4.1 57.7 1.0
CB C:LEU181 4.2 60.8 1.0
CB C:CYS22 4.3 52.3 1.0
CD1 C:LEU181 4.3 60.8 1.0
SG C:CYS190 4.6 55.1 1.0
N C:LEU181 4.7 60.8 1.0
CB C:CYS190 4.8 55.1 1.0
C C:LYS186 4.9 58.6 1.0
CB C:LYS186 4.9 58.6 1.0
SG C:CYS196 5.0 53.2 1.0

Reference:

C.Hu, C.Almendros, K.H.Nam, A.R.Costa, J.N.A.Vink, A.C.Haagsma, S.R.Bagde, S.J.J.Brouns, A.Ke. Mechanism For CAS4-Assisted Directional Spacer Acquisition in Crispr-Cas. Nature V. 598 515 2021.
ISSN: ESSN 1476-4687
PubMed: 34588691
DOI: 10.1038/S41586-021-03951-Z
Page generated: Thu Aug 8 09:16:26 2024

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