Atomistry » Iron » PDB 7mh9-7ni1 » 7mjy
Atomistry »
  Iron »
    PDB 7mh9-7ni1 »
      7mjy »

Iron in PDB 7mjy: Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna

Enzymatic activity of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna

All present enzymatic activity of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna:
2.8.4.3;

Protein crystallography data

The structure of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna, PDB code: 7mjy was solved by O.A.Esakova, T.L.Grove, N.H.Yennawar, A.J.Arcinas, B.Wang, C.Krebs, S.C.Almo, S.J.Booker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.37 / 1.86
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 53.834, 79.982, 110.137, 90, 90, 90
R / Rfree (%) 17.4 / 20.9

Other elements in 7mjy:

The structure of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Iron Binding Sites:

The binding sites of Iron atom in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna (pdb code 7mjy). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 7 binding sites of Iron where determined in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna, PDB code: 7mjy:
Jump to Iron binding site number: 1; 2; 3; 4; 5; 6; 7;

Iron binding site 1 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 1 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe611

b:13.8
occ:1.00
FE1 A:F3S611 0.0 13.8 1.0
S3 A:F3S611 2.2 16.5 1.0
S2 A:F3S611 2.2 17.4 1.0
S1 A:F3S611 2.2 15.7 1.0
SG A:CYS63 2.3 15.4 1.0
FE3 A:F3S611 2.7 14.1 1.0
FE4 A:F3S611 2.7 15.5 1.0
CB A:CYS63 3.2 15.7 1.0
CA A:CYS63 3.7 16.5 1.0
CB A:ILE65 3.8 15.6 1.0
S4 A:F3S611 3.9 16.6 1.0
N A:SER64 4.0 13.7 1.0
N A:ILE65 4.1 13.3 1.0
C A:CYS63 4.3 14.8 1.0
CG2 A:ILE65 4.4 10.7 1.0
CA A:GLY26 4.4 21.2 1.0
CA A:ILE65 4.5 11.3 1.0
N A:CYS27 4.6 17.6 1.0
SG A:CYS97 4.8 14.9 1.0
CG1 A:ILE65 4.8 14.0 1.0
CD1 A:ILE65 4.8 13.6 1.0
SG A:CYS27 4.8 18.5 1.0
CB A:CYS97 4.8 15.2 1.0
O M:HOH103 4.9 39.6 1.0
N A:CYS63 5.0 14.6 1.0

Iron binding site 2 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 2 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe611

b:14.1
occ:1.00
FE3 A:F3S611 0.0 14.1 1.0
S3 A:F3S611 2.2 16.5 1.0
S4 A:F3S611 2.2 16.6 1.0
S1 A:F3S611 2.2 15.7 1.0
SG A:CYS97 2.3 14.9 1.0
FE1 A:F3S611 2.7 13.8 1.0
FE4 A:F3S611 2.8 15.5 1.0
CB A:CYS97 3.2 15.2 1.0
S2 A:F3S611 3.9 17.4 1.0
OG1 A:THR183 4.3 13.2 1.0
CD A:ARG184 4.3 19.3 1.0
O A:HOH743 4.3 22.3 1.0
CG A:ARG184 4.3 15.9 1.0
CA A:CYS63 4.6 16.5 1.0
CA A:CYS97 4.6 13.7 1.0
SG A:CYS63 4.7 15.4 1.0
O A:THR62 4.7 16.3 1.0
CG2 A:VAL180 4.7 10.4 1.0
N A:SER64 4.8 13.7 1.0
SG A:CYS27 4.9 18.5 1.0
CB A:CYS63 4.9 15.7 1.0
N A:CYS97 5.0 15.8 1.0

Iron binding site 3 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 3 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe611

b:15.5
occ:1.00
FE4 A:F3S611 0.0 15.5 1.0
S4 A:F3S611 2.2 16.6 1.0
S3 A:F3S611 2.2 16.5 1.0
S2 A:F3S611 2.2 17.4 1.0
SG A:CYS27 2.3 18.5 1.0
FE1 A:F3S611 2.7 13.8 1.0
FE3 A:F3S611 2.8 14.1 1.0
CB A:CYS27 3.3 16.0 1.0
N A:CYS27 3.7 17.6 1.0
S1 A:F3S611 4.0 15.7 1.0
CA A:CYS27 4.1 15.2 1.0
NE2 A:GLN215 4.6 20.5 1.0
SG A:CYS63 4.8 15.4 1.0
C A:GLY26 4.8 29.2 1.0
SG A:CYS97 4.9 14.9 1.0
CG A:MET29 4.9 33.5 1.0
CB A:MET29 4.9 25.1 1.0
C A:CYS27 5.0 22.8 1.0
CA A:GLY26 5.0 21.2 1.0

Iron binding site 4 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 4 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 4 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe612

b:15.7
occ:1.00
FE1 A:SF4612 0.0 15.7 1.0
S3 A:SF4612 2.3 15.8 1.0
S4 A:SF4612 2.3 14.3 1.0
S2 A:SF4612 2.3 14.7 1.0
SG A:CYS175 2.3 14.3 1.0
FE3 A:SF4612 2.7 14.8 1.0
FE4 A:SF4612 2.7 15.3 1.0
FE2 A:SF4612 2.7 16.4 1.0
CB A:CYS175 3.2 16.2 1.0
NH1 A:ARG293 3.7 12.4 1.0
S1 A:SF4612 3.8 12.7 1.0
N A:CYS175 4.0 15.4 1.0
O A:SAH613 4.0 22.7 1.0
CA A:CYS175 4.2 12.9 1.0
CB A:ASN173 4.3 18.5 1.0
OH A:TYR177 4.3 16.3 1.0
CB A:CYS178 4.5 13.2 1.0
O A:HOH823 4.6 20.4 1.0
SG A:CYS178 4.6 14.0 1.0
SG A:CYS171 4.7 16.3 1.0
CZ A:TYR177 4.9 16.0 1.0
ND2 A:ASN173 5.0 25.9 1.0
N A:PHE174 5.0 12.1 1.0

Iron binding site 5 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 5 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 5 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe612

b:16.4
occ:1.00
FE2 A:SF4612 0.0 16.4 1.0
S3 A:SF4612 2.3 15.8 1.0
S4 A:SF4612 2.3 14.3 1.0
S1 A:SF4612 2.3 12.7 1.0
N A:SAH613 2.4 20.4 1.0
O A:SAH613 2.4 22.7 1.0
FE1 A:SF4612 2.7 15.7 1.0
FE4 A:SF4612 2.8 15.3 1.0
FE3 A:SF4612 2.8 14.8 1.0
CA A:SAH613 3.0 24.0 1.0
C A:SAH613 3.0 20.6 1.0
CB A:SAH613 3.3 25.5 1.0
S2 A:SF4612 3.9 14.7 1.0
O A:HOH738 4.0 20.1 1.0
OXT A:SAH613 4.2 25.2 1.0
CG A:SAH613 4.3 25.0 1.0
NH1 A:ARG293 4.3 12.4 1.0
O A:GLN215 4.4 15.1 1.0
OE1 A:GLN215 4.5 17.1 1.0
SG A:CYS171 4.7 16.3 1.0
CB A:GLN215 4.8 11.6 1.0
SG A:CYS178 4.8 14.0 1.0
SG A:CYS175 4.8 14.3 1.0
O A:HOH719 5.0 24.0 1.0

Iron binding site 6 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 6 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 6 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe612

b:14.8
occ:1.00
FE3 A:SF4612 0.0 14.8 1.0
S2 A:SF4612 2.3 14.7 1.0
S1 A:SF4612 2.3 12.7 1.0
SG A:CYS178 2.3 14.0 1.0
S4 A:SF4612 2.3 14.3 1.0
FE1 A:SF4612 2.7 15.7 1.0
FE4 A:SF4612 2.7 15.3 1.0
FE2 A:SF4612 2.8 16.4 1.0
CB A:CYS178 3.1 13.2 1.0
S3 A:SF4612 3.9 15.8 1.0
CB A:VAL180 4.1 15.3 1.0
CD A:PRO181 4.3 12.9 1.0
CG2 A:VAL180 4.5 10.4 1.0
CA A:CYS178 4.5 12.3 1.0
SG A:CYS175 4.6 14.3 1.0
N A:SAH613 4.7 20.4 1.0
CB A:CYS175 4.7 16.2 1.0
CB A:SAH613 4.7 25.5 1.0
CG A:PRO181 4.8 16.7 1.0
SG A:CYS171 4.9 16.3 1.0
CE1 A:TYR177 4.9 14.0 1.0
N A:VAL180 4.9 17.5 1.0
CG1 A:VAL180 4.9 16.0 1.0
O A:SAH613 5.0 22.7 1.0

Iron binding site 7 out of 7 in 7mjy

Go back to Iron Binding Sites List in 7mjy
Iron binding site 7 out of 7 in the Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 7 of Miab in the Complex with S-Adenosyl-L-Homocysteine and Rna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Fe612

b:15.3
occ:1.00
FE4 A:SF4612 0.0 15.3 1.0
S1 A:SF4612 2.2 12.7 1.0
SG A:CYS171 2.3 16.3 1.0
S2 A:SF4612 2.3 14.7 1.0
S3 A:SF4612 2.3 15.8 1.0
FE1 A:SF4612 2.7 15.7 1.0
FE3 A:SF4612 2.7 14.8 1.0
FE2 A:SF4612 2.8 16.4 1.0
CB A:CYS171 3.3 19.6 1.0
S4 A:SF4612 3.9 14.3 1.0
CB A:ASN173 4.1 18.5 1.0
ND2 A:ASN173 4.3 25.9 1.0
N A:SAH613 4.5 20.4 1.0
CG A:PRO181 4.6 16.7 1.0
CB A:GLN215 4.6 11.6 1.0
CA A:CYS171 4.7 21.2 1.0
N A:ASN173 4.7 18.6 1.0
CG A:ASN173 4.7 21.1 1.0
SG A:CYS175 4.8 14.3 1.0
SG A:CYS178 4.8 14.0 1.0
N A:PHE174 4.9 12.1 1.0
CD A:PRO181 4.9 12.9 1.0
O A:SAH613 4.9 22.7 1.0
CA A:ASN173 4.9 20.6 1.0

Reference:

O.A.Esakova, T.L.Grove, N.H.Yennawar, A.J.Arcinas, B.Wang, C.Krebs, S.C.Almo, S.J.Booker. Structural Basis For Trna Methylthiolation By the Radical Sam Enzyme Miab Nature 2021.
ISSN: ESSN 1476-4687
DOI: 10.1038/S41586-021-03904-6
Page generated: Thu Aug 8 09:18:07 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy