Iron in PDB 7ok0: Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A

All present enzymatic activity of Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A:
2.7.7.6;

The structure of Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Zinc	(Zn)	6 atoms

The binding sites of Iron atom in the Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A (pdb code 7ok0). This binding sites where shown within 5.0 Angstroms radius around Iron atom.
In total 3 binding sites of Iron where determined in the Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A, PDB code: 7ok0:
Jump to Iron binding site number: 1; 2; 3;

Iron binding site 1 out of 3 in the Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 1 of Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe301 b:73.8 occ:1.00 FE1 D:F3S301 0.0 73.8 1.0 S3 D:F3S301 2.2 73.8 1.0 S1 D:F3S301 2.2 73.8 1.0 S2 D:F3S301 2.2 73.8 1.0 SG D:CYS209 2.3 73.3 1.0 FE3 D:F3S301 3.1 73.8 1.0 FE4 D:F3S301 3.1 73.8 1.0 CB D:CYS209 3.4 73.3 1.0 SG D:CYS206 3.5 62.1 1.0 N D:CYS209 3.5 73.3 1.0 N D:GLU208 3.9 69.5 1.0 N D:GLU207 4.0 68.0 1.0 CA D:CYS209 4.1 73.3 1.0 S4 D:F3S301 4.2 73.8 1.0 SG D:CYS203 4.2 61.1 1.0 CA D:GLU207 4.3 68.0 1.0 CD1 D:ILE219 4.3 73.7 1.0 C D:GLU207 4.5 68.0 1.0 CG1 D:ILE219 4.6 73.7 1.0 C D:GLU208 4.6 69.5 1.0 CA D:GLU208 4.7 69.5 1.0 CE2 D:PHE188 4.8 74.7 1.0 C D:CYS206 5.0 62.1 1.0

Iron binding site 2 out of 3 in the Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 2 of Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe301 b:73.8 occ:1.00 FE3 D:F3S301 0.0 73.8 1.0 S3 D:F3S301 2.2 73.8 1.0 S1 D:F3S301 2.2 73.8 1.0 S4 D:F3S301 2.2 73.8 1.0 SG D:CYS203 2.3 61.1 1.0 SG D:CYS206 2.3 62.1 1.0 FE4 D:F3S301 3.0 73.8 1.0 FE1 D:F3S301 3.1 73.8 1.0 N D:CYS206 3.4 62.1 1.0 CB D:CYS206 3.7 62.1 1.0 CB D:CYS203 3.9 61.1 1.0 N D:ILE204 4.0 55.9 1.0 N D:LEU205 4.0 56.5 1.0 CA D:CYS206 4.1 62.1 1.0 CA D:CYS203 4.2 61.1 1.0 S2 D:F3S301 4.2 73.8 1.0 N D:GLU207 4.3 68.0 1.0 C D:LEU205 4.4 56.5 1.0 CA D:LEU205 4.4 56.5 1.0 C D:CYS203 4.5 61.1 1.0 SG D:CYS183 4.6 67.4 1.0 C D:ILE204 4.7 55.9 1.0 C D:CYS206 4.7 62.1 1.0 CG1 D:ILE204 4.7 55.9 1.0 CA D:ILE204 4.9 55.9 1.0

Iron binding site 3 out of 3 in the Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A


Mono view


Stereo pair view

A full contact list of Iron with other atoms in the Fe binding site number 3 of Cryo-Em Structure of the Sulfolobus Acidocaldarius Rna Polymerase at 2.88 A within 5.0Å range: probe atom residue distance (Å) B Occ D:Fe301 b:73.8 occ:1.00 FE4 D:F3S301 0.0 73.8 1.0 S3 D:F3S301 2.2 73.8 1.0 S2 D:F3S301 2.2 73.8 1.0 S4 D:F3S301 2.2 73.8 1.0 SG D:CYS183 2.3 67.4 1.0 CB D:CYS183 2.5 67.4 1.0 FE3 D:F3S301 3.0 73.8 1.0 CA D:CYS183 3.0 67.4 1.0 FE1 D:F3S301 3.1 73.8 1.0 SG D:CYS206 3.4 62.1 1.0 CD D:PRO184 4.0 62.0 1.0 C D:CYS183 4.0 67.4 1.0 N D:CYS183 4.2 67.4 1.0 S1 D:F3S301 4.2 73.8 1.0 N D:PRO184 4.2 62.0 1.0 SG D:CYS203 4.5 61.1 1.0 CE2 D:PHE188 4.5 74.7 1.0 SG D:CYS209 4.8 73.3 1.0 O D:CYS183 4.9 67.4 1.0 N D:GLU185 4.9 64.5 1.0 CG2 D:VAL187 5.0 71.1 1.0 CB D:CYS209 5.0 73.3 1.0 CD2 D:PHE188 5.0 74.7 1.0

S.Pilotto, T.Fouqueau, N.Lukoyanova, C.Sheppard, S.Lucas-Staat, L.M.Diaz-Santin, D.Matelska, D.Prangishvili, A.C.M.Cheung, F.Werner. Structural Basis of Rna Polymerase Inhibition By Viral and Host Factors Nat Commun 2021.
ISSN: ESSN 2041-1723